Disease relevance of DopR

• In addition, the larval abdominal ganglion contained two dDA1 cells in each segment [1].

High impact information on DopR

• DAMB, a novel dopamine receptor expressed specifically in Drosophila mushroom bodies [2].
• Agonist-specific coupling of a cloned Drosophila melanogaster D1-like dopamine receptor to multiple second messenger pathways by synthetic agonists [3].
• This is the first evidence for the direct coupling of a cloned D1-like dopamine receptor both to the activation of adenylyl cyclase and to the initiation of an intracellular Ca2+ signal [3].
• Invertebrate D2 type dopamine receptor exhibits age-based plasticity of expression in the mushroom bodies of the honeybee brain [4].
• The transmembrane domains of this receptor are 88% identical to the orthologous Drosophila D2 dopamine receptor, DmDop2, though phylogenetic analysis and sequence homology both indicate that invertebrate and vertebrate D2 receptors are quite distinct [4].

Biological context of DopR

• We report here the isolation from Drosophila melanogaster of a 2.0 kb cDNA clone encoding a 385 amino acid protein (dDA1) displaying, within putative transmembrane domains, highest amino acid sequence homology (49-53%) to members of the vertebrate dopamine D1-like receptor family [5].
• Here we describe the structural and functional properties of the promoter region of a dopamine receptor-gene (Dmdop1) from Drosophila [6].
• Characterization of the 5' regulatory region of the Drosophila Dmdop1 dopamine receptor-gene [6].
• As a first step towards physiological expression studies we isolated a D1-like dopamine receptor from a tilapia hypothalamus cDNA library [7].
• In the meantime, the DAI muscle founders were absent in some segments in svp mutant embryos, and the dorsal somatic muscle patterning was also severely damaged in the late stage mutant embryos, suggesting that svp is required for the differentiation of Eve-positive pericardial cells and DA1 muscle founders and may have a role in EPC cell growth [8].

Anatomical context of DopR

• The mechanism of coupling of a cloned Drosophila D1-like dopamine receptor, DopR99B, to multiple second messenger systems when expressed in Xenopus oocytes is described [3].
• In situ hybridization revealed that dDA1 receptor mRNA is expressed as a maternal transcript, and at later blastoderm stages is restricted to apical regions of the cortical peripheral cytoplasm [5].
• When expressed in either Sf9 or COS-7 cells, dDA1 did not bind the specific D1-like receptor antagonist [3H]SCH-23390 or numerous other dopaminergic, adrenergic or serotoninergic ligands with high affinity [5].
• In situ hybridization revealed that the Drosophila dopamine receptor is highly expressed in the somata of the optic lobes [9].
• Expression of a D1 dopamine receptor dDA1/DmDOP1 in the central nervous system of Drosophila melanogaster [1].

Associations of DopR with chemical compounds

• However, like vertebrate dopamine D1-like receptors, dDA1 stimulated the accumulation of cAMP in response to DA (EC50 approximately 300 nM) and 6,7-ADTN (EC50 approximately 500 nM) [5].

Analytical, diagnostic and therapeutic context of DopR

• One new receptor, DAMB, was identified as a dopamine D1 receptor by sequence analysis and pharmacological characterization [2].
• Because DopR99B is the second dopamine receptor cloned from Drosophila, this work establishes dopamine receptor diversity in a system amenable to genetic dissection [10].

References