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Gene Review

Pp1alpha-96A  -  Protein phosphatase 1alpha at 96A

Drosophila melanogaster

Synonyms: 96A, CG6593, DmPp1-96A, Dmel\CG6593, PP1, ...
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High impact information on Pp1alpha-96A

  • In contrast, expression of transgenes encoding the Bif F995A mutant, which disrupts binding to PP1, was unable to rescue any aspect of the bif phenotype [1].
  • The gene bifocal (bif), required for photoreceptor morphogenesis in the Drosophila compound eye, encodes a protein that is shown to interact with protein phosphatase 1 (PP1) using the yeast two-hybrid system [1].
  • Furthermore, human activated Aurora-A forms complexes with the negative regulator protein serine/threonine phosphatase type 1 (PP1) that was negatively phosphorylated on Thr320 [2].
  • The D. melanogaster Pp1-96A-Acr96Aa segment conserved in D. repleta and D. buzzatii is longer than previously thought and is also conserved in D. virilis [3].
  • PP1 beta shows 85% identity to PP1 alpha 1 and PP1 alpha 2 over the 302 homologous amino acids [4].

Biological context of Pp1alpha-96A

  • The gene for Drosophila PP1 alpha 1 is at 96A2-5 on chromosome 3 and encodes a protein of 327 amino acids with a calculated molecular mass of 37.3 kDa [4].
  • The sequence of Drosophila PP1 alpha 1 was deduced from a 2.2-kb cDNA purified from an embryonic cDNA library, while that for Drosophila PP1 beta was obtained from overlapping clones isolated from both a head cDNA library and an eye imaginal disc cDNA library [4].
  • Isolation of a further cDNA clone, hybridising to 9C1-2 and encoding part of the catalytic subunit 88% similar to Drosophila PP-1 alpha, proves the existence of at least two transcriptionally active genes for protein phosphatase 1 [5].
  • Drosophila is an excellent model organism in which to characterize the role of PP1 catalytic and regulatory subunits, because it combines molecular and biochemical approaches with powerful genetics, in a well-characterized animal model [6].
  • Regulatory subunits of PP1 provide the key to understanding the role of PP1, as they are responsible for directing PP1c to different intracellular locations and/or affecting their activity or substrate specificity [6].

Anatomical context of Pp1alpha-96A


Associations of Pp1alpha-96A with chemical compounds


  1. Interaction with protein phosphatase 1 Is essential for bifocal function during the morphogenesis of the Drosophila compound eye. Helps, N.R., Cohen, P.T., Bahri, S.M., Chia, W., Babu, K. Mol. Cell. Biol. (2001) [Pubmed]
  2. Phospho-regulation of human protein kinase Aurora-A: analysis using anti-phospho-Thr288 monoclonal antibodies. Ohashi, S., Sakashita, G., Ban, R., Nagasawa, M., Matsuzaki, H., Murata, Y., Taniguchi, H., Shima, H., Furukawa, K., Urano, T. Oncogene (2006) [Pubmed]
  3. Comparative mapping of cosmids and gene clones from a 1.6 Mb chromosomal region of Drosophila melanogaster in three species of the distantly related subgenus Drosophila. Ranz, J.M., Cáceres, M., Ruiz, A. Chromosoma (1999) [Pubmed]
  4. Drosophila contains three genes that encode distinct isoforms of protein phosphatase 1. Dombrádi, V., Axton, J.M., Brewis, N.D., da Cruz e Silva, E.F., Alphey, L., Cohen, P.T. Eur. J. Biochem. (1990) [Pubmed]
  5. Cloning and chromosomal localization of Drosophila cDNA encoding the catalytic subunit of protein phosphatase 1 alpha. High conservation between mammalian and insect sequences. Dombrádi, V., Axton, J.M., Glover, D.M., Cohen, P.T. Eur. J. Biochem. (1989) [Pubmed]
  6. Yeast Two-Hybrid Screens to Identify Drosophila PP1-Binding Proteins. Bennett, D., Alphey, L. Methods Mol. Biol. (2006) [Pubmed]
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