High impact information on Sce

• Posterior sex combs (PSC), a subunit of dPCC, inhibits chromatin remodeling and transcription efficiently but requires assembly with dRING1 to recruit chromatin [1].
• However, Ring chromosome recovery is dramatically reduced in ord oocytes compared with wild type, which is consistent with the model that defects in meiotic cohesion remove the constraints that normally limit recombination between sisters [2].
• The CP2-dinG interaction is conserved in evolution with the Drosophila factor grainyhead binding to dring, the fly homologue of dinG [3].
• Eight mutations were found in Ding, a previously uncharacterized gene with sequence similarity to the Saccharomyces cerevisiae Pds1, whose product is essential for proper chromosome disjunction [4].
• A putative participant in the regulation of this process is the murine RYBP (Ring and YY1 Binding Protein) gene [5].

Biological context of Sce

• We show that a missense mutation in ORD completely ablates the two-hybrid interaction with dRING and prevents nuclear retention of the mutant ORD protein in male meiotic cells [6].
• Using affinity-purified antibodies generated against full-length recombinant dRING, we demonstrate that dRING protein is expressed in the male and female gonads and colocalizes extensively with ORD on the chromatin of primary spermatocytes during G2 of meiosis [6].
• Reaper and Hid induce cell death in a Ring-dependent manner by stimulating Diap1 auto-ubiquitination and degradation [7].

Anatomical context of Sce

• Photographic maps of the salivary gland chromosomes are presented, as well as data on their most prominent puffs during late larval and white prepupal development and, after in vitro ecdysone treatment, their reverse tandem duplications, and the well-formed Balbiani ring 1 [8].

References