Disease relevance of Mcm3ap

• These results suggest that GANP overexpression is a causative factor in the generation of B lymphomas [1].

High impact information on Mcm3ap

• G5PR associates with GANP, whose expression is up-regulated in mature B cells of the peripheral lymphoid organs [2].
• B cell-specific ganp-deficient mice showed a decreased frequency of variable-region somatic mutations, especially of the high-affinity type (W(33) --> L) in the V(H)186.2 region against nitrophenyl-chicken gamma globulin, whereas the class switching was normal [3].
• A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication [4].
• The expression of GANP was strikingly correlated with GC formation because Bcl6-deficient mice did not show the up-regulation of GANP [4].
• GANP is a 210-kd protein localized in both the cytoplasm and nuclei, with a homologous region to Map80 that is associated with MCM3, a protein essential for DNA replication [4].

Biological context of Mcm3ap

• In the study reported here, we have shown abnormal up-regulation of germinal center B cell-associated GANP in various human lymphomas including mantle cell, diffuse large B cell, and Hodgkin lymphoma, by immunohistochemical analysis [1].
• To study the role of GANP in lymphomagenesis, we generated mutant mice (ganp-Tg) that express the transgenic ganp gene under immunoglobulin enhancer and promoter control [1].
• Germinal center-associated DNA primase (GANP) associated with MCM3 of the DNA replication complex is up-regulated selectively in germinal center B cells [5].
• Selective regulation of ganp expression was observed in the -737-bp promoter region in B and plasma cell lines but was significantly low in pre-B and T cell lines [5].
• GANP-positive cells appeared in the light zone of the GC, with coexpression of the peanut agglutinin (PNA) (PNA)-positive B220-positive phenotype [4].

Anatomical context of Mcm3ap

• In this study, we examined the expression of GANP in lymphoid tissues of autoimmune-prone mice [6].
• GANP expression was up-regulated in GC-B cells after stimulation with TD-Ags; however, highly GANP-positive (GANP(hi)) cells were also observed in lymph nodes of non-immunized MRL/lpr mice [6].
• GANP-positive cells were mostly surrounded by follicular dendritic cells [4].
• We found that GANP was up-regulated in GC B cells of Peyer's patches in normal mice and in spleens from Ag-immunized mice [4].

Other interactions of Mcm3ap

• GANP promoter activity was higher in wild-type vs Lyn-deficient cells [7].

References