Disease relevance of ROBO4

• Robo1/Robo4: Differential expression of angiogenic markers in colorectal cancer [1].
• Serum specificity was established first by examining antigen smears of T. pallidum subsp. pallidum, T. pallidum subsp. pertenue, B. burgdorferi, T. phagedenis, and Treponema denticola MRB and then by examining tissues infected with these pathogens plus those infected with four Leptospira serovars [2].
• Serum levels of Magic Roundabout protein in patients with advanced non-small cell lung cancer (NSCLC) [3].

High impact information on ROBO4

• Furthermore, Robo4-expressing endothelial cells show morphology and phenotype, characteristic of Rho GTPase activation [4].
• Moreover, Robo4 is specifically expressed in the vascular endothelium during murine embryonic development [5].
• Magic roundabout, a tumor endothelial marker: expression and signaling [6].
• RESULTS: Two major forms of human MR (hMR), named MRA and MRB, generated by alternative initiation of translation from start methionine 1 and 15 respectively, were predicted [7].
• For Robo4, an up-regulation was detected in >70% (36/50) [1].

Biological context of ROBO4

• Muscarinic (cholinergic) receptor binding sites (MRB) were studied by determining the 3H-QNB binding in four cortical areas and hippocampus of 20 histologically confirmed Alzheimer patients and comparing these with corresponding controls [8].

Anatomical context of ROBO4

• We therefore hypothesize that expression of MRB is involved in regulating the migration of endothelial cells during tumor angiogenesis [6].
• From our baboon studies the average DVR for thalamus (n = 18) for SS-MRB is 1.8; however, the lower limit is most likely less than 1 due to this difference in non-NET binding [9].
• (2) The occipital cortex and the basal ganglia were found to be the regions with the smallest change between baseline (SS-MRB) and pretreatment with cocaine, and were therefore used as a composite reference region for calculation of a distribution volume ratio (DVR) [9].

Associations of ROBO4 with chemical compounds

• Its methyl analogue, 2-[alpha-(2-methoxyphenoxy)benzyl]morpholine (MRB), has been radiolabeled with C-11 for studies of the NET system with positron emission tomography (PET) [10].
• MATERIAL AND METHODS: The adhesive primers Acryl Bond (AB), Cesead Opaque Primer (COP), Metal Primer II (MPII), and MR Bond (MRB) were used [11].

Analytical, diagnostic and therapeutic context of ROBO4

• The overexpression of Robo1 and Robo4 in tumor vs. normal tissue was verified using real-time PCR [1].
• These monoclonal antibodies did not react with nonpathogenic treponemes, such as T. phagedenis Reiter, T. denticola MRB, T. refringens Noguchi, or other spirochetes, such as Borrelia burgdorferi and Leptospira interrogans serovar pomona in microimmunofluorescent antibody smear slides or in Western blots (immunoblots) [12].

References