Disease relevance of Slc15a1

• Periodontal bone loss in mice orally inoculated with Peptostreptococcus anaerobius, Pept. magnus and Actinobacillus actinomycetemcomitans was compared to that in sham-inoculated mice [1].

High impact information on Slc15a1

• Peptide transporter molecules and other MHC-linked gene products have been implicated in the generation and import of antigenic peptides into the lumen of the endoplasmic reticulum for assembly with MHC class I glycoproteins [2].
• This PECT chimera was stably transfected into two PC2-expressing cell lines, AtT-20/PC2 and Rin cells [3].
• Peptide transporter-independent, stress protein-mediated endosomal processing of endogenous protein antigens for major histocompatibility complex class I presentation [4].
• Furthermore, treatment of the human intestinal model, Caco-2 cells, with WY-14643 resulted in enhanced PEPT1 mRNA expression and uptake activity of glycylsarcosine [5].
• In the small intestine of PPARalpha-null mice, augmentation of PEPT1 mRNA during fasting was completely abolished [5].

Biological context of Slc15a1

• The mouse PEPT1 cDNA cloned from a kidney cDNA library is approximately 3.1 kb long and encodes a protein of 709 amino acids [6].
• The mouse pept1 gene, cloned from a genomic DNA library in bacterial artificial chromosome, is approximately 38 kb long and consists of 23 exons and 22 introns [6].
• Functional analysis of the promoter region using the luciferase reporter assay in Caco-2 cells (a human intestinal cell line that express PEPT1 constitutively) and five different 5'-deletion fragments of the promoter has shown that essential promoter/enhancer elements are present within 1140 bp upstream of the transcription start site [6].
• We previously demonstrated that starvation markedly increased the amount of mRNA and protein levels of the intestinal H(+)/peptide cotransporter (PEPT1) in rats, leading to altered pharmacokinetics of the PEPT1 substrates [5].

Anatomical context of Slc15a1

• When expressed heterologously in mammalian cells and in Xenopus laevis oocytes, mouse PEPT1 mediates H(+)-coupled electrogenic transport of the dipeptide glycylsarcosine [6].
• Immunohistochemistry for PEPT1 localized immunoreactivity to the enterocytes, with the highest intensity at the apical membrane [7].
• Induction of intestinal peptide transporter 1 expression during fasting is mediated via peroxisome proliferator-activated receptor {alpha} [5].
• The nature of protein breakdown products and peptidomimetic drugs such as beta-lactams is crucial for their transmembrane transport across apical enterocyte membranes, which is accomplished by the pH-dependent high-capacity oligopeptide transporter PEPT1 [7].

Associations of Slc15a1 with chemical compounds

• The ability of ATB(0,+) to transport valacyclovir was comparable with that of the peptide transporter PEPT1 [8].

Analytical, diagnostic and therapeutic context of Slc15a1

• Oral administration of the synthetic PPARalpha ligand WY-14643 to fed rats increased the mRNA level of intestinal PEPT1 [5].

References