Disease relevance of Hspa2

• Pretreatment with HST extract markedly inhibited the reduction of gastric mucin content and the development of gastric ulcer with significant differences (p < or = 0.01) [1].
• These results suggest that HST might suppress inflammatory bowel disease (IBD) and imply that there will be a potential benefit in the traditionally derived herbal combination [2].
• The aim of the present study is to clarify anti-colitic effect of HST using a model of colitis induced by intracolonic instillation of 2,4,6-trinitrobenzene sulfonic acid (TNBS) in rats, and to evaluate the pharmaceutical properties of its herbal components [2].

High impact information on Hspa2

• Structure of the 5' region of the Hst70 gene transcription unit: presence of an intron and multiple transcription initiation sites [3].
• Functional analysis of the Hst70 gene promoter in transgenic mice and transient transfection assays proved that the DNA fragment of approx. 360 bp localized upstream of the ATG transcription start codon is the minimal promoter required for testis-specific expression of the HST70/chloramphenicol acetyltransferase transgene [3].
• The levels of activity of intestinal disaccharidases of upper (proximal one-third) and lower (middle one-third) jejunum, and pancreatic alpha-amylase were lower in rats fed LST diet in both age groups than in rats fed HST [4].
• We have examined the effects of hypophysectomy and treatment with thyroxine (T4) on enzyme activity and expression (as determined by immunoblot analysis) of members of the three principal sulfotransferase (ST) sub-families (phenol STs, PST; estrogen STs, EST; hydroxysteroid STs, HST) in cytosols prepared from female Wistar rat livers [5].
• Electrophoretic mobility-shift assay analysis revealed that only testes of juvenile rats, when Hst70 gene expression is repressed, contain proteins that specifically bind to the Oct (octamer) sequence localized directly downstream of the T1 site [6].

Anatomical context of Hspa2

• Immunoblot analysis of these cytosols with antibodies recognising HST and PST indicated that where changes in enzyme activity occurred they mirrored changes in enzyme protein expression [5].
• Those changes in monoamine contests in hypothalamus were also significantly inhibited by the pretreatment with HST extract at higher dosage (p < or = 0.05) [1].

Analytical, diagnostic and therapeutic context of Hspa2

• Hange-shashin-to (HST) has been used as an herbal formula to treat inflammatory ulcerative gut diseases complicated with psychoneurosis in Japanese traditional Kampo medicine [2].

References