Disease relevance of Igk-V21-4

• We have previously reported that a segment of DNA from a murine plasmacytoma comprises DNA from three chromosomes, the immunoglobulin kappa light-chain locus on chromosome 6, the S mu locus on chromosome 12, and a region on chromosome 15 [1].
• The transcription factor NF-kappa B has been implicated as an important regulator of cellular and viral genes, including those of immunoglobulin kappa light chain, interleukin-2, beta-interferon, HIV-1 and cytomegalovirus [2].
• This has been tested in the B16F10 murine melanoma model by treating B16F10-bearing C57BL/6 mice either with a CD44s-/ CD44v10-specific antibody, or with receptor globulins (Rg) containing the extracellular part of CD44s or CD44v10 linked to the constant region of the immunoglobulin kappa light chain [3].

High impact information on Igk-V21-4

• Delayed cellular maturation and decreased immunoglobulin kappa light chain production in immature B lymphocytes lacking B cell linker protein [4].
• In situ hybridization techniques were used to detect expression within lymphoid tissues of genes encoding T cell receptor (TCR) alpha, beta and gamma chains, as well as immunoglobulin kappa light chain [5].
• In mature mouse B lymphocytes, immunoglobulin kappa light chain transcripts contain an intervening sequence separating the recombined variable (V) plus joining (J) exon from the distant constant (C) exon [6].
• The pi enhancer element is transcriptionally active primarily during early stages of B-cell development but becomes virtually inactive during B-cell maturation at about the stage of immunoglobulin kappa light-chain gene rearrangement [7].
• The mouse immunoglobulin kappa light-chain genes are located in early- and late-replicating regions of chromosome 6 [8].

Biological context of Igk-V21-4

• The results indicate species-related functional differences in the immunoglobulin kappa light chain gene enhancer and suggest that although the NF-kappa B binding site plays a crucial role in enhancer activity surrounding gene elements are also necessary for full enhancer effect [9].
• A plasmid encoding an IgK leader peptide-driven secretable fusion protein of the active GLP-1 and IgG(1)-Fc was constructed for mammalian expression [10].

Anatomical context of Igk-V21-4

• Expression of an immunoglobulin kappa light-chain gene in lymphoid cells using a bovine papillomavirus-1 (BPV-1) vector [11].
• We have tested such a vector for the expression in hybridoma cells of the immunoglobulin kappa light-chain gene, but found that the level of expression varies greatly among transformants [11].

Other interactions of Igk-V21-4

• In murine cells chronically infected with M-MuLV, gel shift analyses with kappaB DNA-binding motifs from the murine immunoglobulin kappa light chain enhancer demonstrated induction of at least two distinct kappaB enhancer-binding complexes [12].

References