High impact information on SNIP1

• SNIP1 gene was first identified through a yeast two-hybrid system, using Smad1 as the bait [1].
• The carboxyl terminus of SNIP1 interacts with Smad1 and Smad2 in yeast two-hybrid as well as in mammalian overexpression systems [2].
• Overexpression of full-length SNIP1 or its amino terminus is sufficient to inhibit multiple gene responses to TGF-beta and CBP/p300, as well as the formation of a Smad4/p300 complex [2].
• Thus, SNIP1 is a nuclear inhibitor of CBP/p300 and its level of expression in specific cell types has important physiological consequences by setting a threshold for TGF-beta-induced transcriptional activation involving CBP/p300 [2].
• Studies in Xenopus laevis further suggest that SNIP1 plays a role in regulating dorsomedial mesoderm formation by the TGF-beta family member nodal [2].
• A novel smad nuclear interacting protein, SNIP1, suppresses p300-dependent TGF-beta signal transduction [2].

Biological context of SNIP1

• Surprisingly, we found that reduction in SNIP1 levels resulted in significantly reduced cell proliferation and accumulation of cells in the G1 phase of the cell cycle [3].
• Smad nuclear interacting protein 1 (SNIP1) is an evolutionarily conserved protein containing a forkhead-associated (FHA) domain that regulates gene expression through interactions with multiple transcriptional regulators [3].
• Moreover, SNIP1 depletion results in inhibition of cyclin D1 promoter activity in a manner dependent upon a previously characterized binding site for the AP-1 transcription factor family [3].
• Using a yeast two-hybrid screen, we found that SNIP1 (Smad nuclear-interacting protein 1) associates with c-Myc, a key regulator of cell proliferation and transformation [4].

Anatomical context of SNIP1

• Here, we have used short interfering RNAs (siRNAs) to knockdown SNIP1 expression in human cell lines [3].

Associations of SNIP1 with chemical compounds

• SNIP1 Is a Candidate Modifier of the Transcriptional Activity of c-Myc on E Box-Dependent Target Genes [4].
• The influence of p300 on LHbeta promoter is potentiated by steroid receptor co-activator, as well as by E1A proteins, and attenuated by Smad nuclear interacting protein 1 [5].

Regulatory relationships of SNIP1

• SNIP1 itself is induced upon serum stimulation immediately prior to cyclin D1 expression [3].

Other interactions of SNIP1

• SNIP1 is a recently discovered novel repressor of CBP/p300 [6].
• The patent number associated with the production of SNIP1 antibody is US6906179.

References