High impact information on PF10_0343

• The 33 bp tandem repeats encoded by the FC27 S-antigen gene could not be detected in the NF7 genome [1].
• Conserved sequences flank variable tandem repeats in two S-antigen genes of Plasmodium falciparum [1].
• The size heterogeneity results from different numbers, lengths, and/or sequences of tandem repeat units encoded within the S-antigen genes [2].
• Changes in repeat number, sequence, and reading frame in S-antigen genes of Plasmodium falciparum [2].
• Live recombinant viruses induced very poor antibody responses to a secreted repetitive plasmodial antigen (the S-antigen) in rabbits and mice [3].

Biological context of PF10_0343

• This is observed in the highly repetitive sequences of some Plasmodium antigens (S-antigen, CS proteins) [4].
• In addition, a subline of FUP/C strain selected by repeated flotation on gelatin was found to differ in several characters such as KHARP, P190 and S-antigen genes, which are known to be located on different chromosomes [5].
• Several bands were relatively weakly labelled with 3H-glycine, suggesting that natural isolates contain one or two predominant S-antigen phenotypes and several other S-antigen phenotypes expressed by minor parasite subpopulations [6].
• We describe Plasmodium falciparum genetic diversity in coastal Kenya, typing S-antigen and the merozoite surface proteins 1 and 2 (MSP-1 and MSP-2) in field isolates by the polymerase chain reaction (PCR) [7].
• Mutations in non-repetitive regions of the S-antigen and in AMA-1, an antigen lacking repeats, are strongly biased towards those which alter the amino acid sequence [8].

Anatomical context of PF10_0343

• The S-antigen of Plasmodium falciparum is a highly diverse heat stable protein that is located in the parasitophorous vacuole of the mature asexual intraerythrocytic parasite [9].
• Cross-sectional and longitudinal village-based studies of the transmission dynamics of an S-antigen serotype of the asexual erythrocyte stages of Plasmodium falciparum have been carried out in Madang, Papua New Guinea (PNG) [10].

Associations of PF10_0343 with chemical compounds

• The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained [11].
• Chemical crosslinking of Plasmodium falciparum glycoprotein, Pf200 (190-205 kDa), to the S-antigen at the merozoite surface [12].

Other interactions of PF10_0343

• We describe the use of the Mantel test for assessing clustering of individual parasite alleles at the household level, and demonstrate low-level clustering of MSP-1 and MSP-2 alleles and S-antigen sequence types, at the end of a long period of low transmission [7].
• The glycophorin-binding protein, the histidine-rich protein II, the S-antigen, the heat shock protein 70, the ring-infected erythrocyte surface antigen, and the apical membrane antigen-1 were also shown serologically to be present in the mixture of exoantigens [13].

Analytical, diagnostic and therapeutic context of PF10_0343

• The characteristics of the S antigen, defined by immunoblotting, allowed it to be identified in two-dimensional separations of [35S]methionine-labeled parasite proteins, thus confirming the parasite origin of the antigen [14].
• The S-antigen of clone C-10 was resolved on SDS/PAGE as two labelled products with molecular weights of 156000 +/- 5000 and 130000 +/- 2000 [15].
• In other experiments, boiled plasma from Aotus monkeys infected with a different isolate of P. falciparum was partially purified by isoelectric focusing and the fraction containing S-antigen was iodinated by the Iodogen procedure [15].
• Sera collected from village residents were screened for circulating S-antigen of the FC27 serotype by enzyme-linked immunosorbent assay (ELISA) [10].
• Localization of the S-antigen of Plasmodium falciparum isolate FCQ27/PNG, from Papua New Guinea, was studied by post-embedding immunoelectron microscopy using affinity-purified rabbit antibodies raised against the repeat region of the antigen [16].

References