High impact information on HMG2

• Expression of the Arabidopsis HMG2 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, is restricted to meristematic and floral tissues [1].
• The transcriptional activity of the HMG2 gene was studied after fusing different regions of its 5' flanking region to the beta-glucuronidase (GUS) reporter gene and transforming the resulting constructs into tobacco plants [1].
• Deletion analysis of the HMG2 5' flanking region was conducted in transgenic plants and transfected protoplasts [1].
• Inoculation with a compatible race of P. infestans resulted in similar patterns in HMGR gene expression of hmg2 and hmg3 except that the magnitude and rate of the changes in mRNA levels were reduced relative to the incompatible interaction [2].
• The hmg2 and hmg3 mRNAs also accumulated in response to wounding, but in contrast to hmg1, these mRNAs were strongly enhanced by arachidonic acid or inoculation [2].

Biological context of HMG2

• HMG1 and HMG2 consist of four exons and three small introns that interrupt the coding sequence at equivalent positions [3].
• The similarity between the two encoded proteins (HMGR1 and HMGR2) is restricted to the regions corresponding to the membrane and the catalytic domains [3].
• The hmg2 mutant showed no visible phenotype under normal growth conditions [4].

Anatomical context of HMG2

• By using a coupled in vitro transcription-translation assay, we show that both HMGR1 and HMGR2 are cotranslationally inserted into endoplasmic reticulum-derived microsomal membranes [3].

Associations of HMG2 with chemical compounds

• The suppression of hmg1 and the enhancement of hmg2 and hmg3 transcript levels following elicitor treatment or inoculation with the incompatible race parallel the suppression in steroid and stimulation of sesquiterpenoid accumulations observed in earlier investigations [2].
• The hmg1 and hmg2 mutants were both more sensitive than the wild type (WT) to lovastatin, an inhibitor of HMGR [4].

Other interactions of HMG2

• HMG1 mRNA is detected in all tissues, whereas the presence of HMG2 mRNA is restricted to young seedlings, roots, and inflorescences [3].

Analytical, diagnostic and therapeutic context of HMG2

• Sequence analysis revealed that these clones fall into two different classes, HMGR1 and HMGR2 [5].

References