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Gene Review

PEP3  -  Pep3p

Saccharomyces cerevisiae S288c

Synonyms: Carboxypeptidase Y-deficient protein 3, L9634.2, VAM8, VPS18, VPT18, ...
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Disease relevance of PEP3


High impact information on PEP3

  • Together with the similar phenotypes exhibited by both vps11 and vps18 mutants, this finding suggests that they may function at a common step during vacuolar protein sorting and that the integrity of their zinc finger motifs may be required for this function [3].
  • Most vps18 mutants are temperature sensitive for growth and are defective in vacuole biogenesis; no structure resembling a normal vacuole is seen [3].
  • Disruption of the VPS18/PEP3 gene (vps18 delta 1::TRP1) is not lethal but results in the same vacuolar protein sorting and growth defects exhibited by the original temperature-sensitive vps18 alleles [3].
  • A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation [3].
  • This finding suggests that vps18 mutations alter the function of a late Golgi compartment which contains Kex2p and in which vacuolar proteins are thought to be sorted from proteins destined for the cell surface [3].

Biological context of PEP3

  • Integrative mapping experiments indicated that the 26-kb insert in this plasmid was derived from the VPS18 locus [3].
  • The DNA sequence of the fragment was determined; a 2,754-bp open reading frame predicts that the PEP3 gene product is a hydrophilic, 107-kDa protein that has no significant similarity to any known protein [1].
  • The Saccharomyces cerevisiae PEP3 gene was cloned from a wild-type genomic library by complementation of the carboxypeptidase Y deficiency in a pep3-12 strain [1].
  • In this study we demonstrate that proteins encoded by genes previously demonstrated to play critical roles in vacuole assembly for acidification, PEP3, PEP5 and VMA3, are also required for normal copper and iron metal ion homeostasis [4].
  • The gene could also suppress the temperature sensitivity of the htr1 disruptant (Kikuchi et al. (1994) Mol. Gen. Genet. 245, 107-116) and was physically mapped in the region near PEP3 on chromosome XII R [5].

Anatomical context of PEP3


Associations of PEP3 with chemical compounds

  • The Vps18p sequence contains a cysteine-rich, zinc finger-like motif at the COOH terminus [3].
  • Yeast cells lacking a functional PEP3 or PEP5 gene are hypersensitive to copper and render the normally iron-repressible FET3 gene, encoding a multi-copper Fe(II) oxidase involved in Fe2+ transport, also repressible by exogenous copper ions [4].

Physical interactions of PEP3

  • Our results suggest that a core Pep3p/Pep5p complex promotes vesicular docking/fusion reactions in conjunction with SNARE proteins at multiple steps in transport routes to the vacuole [7].


  1. Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae. Preston, R.A., Manolson, M.F., Becherer, K., Weidenhammer, E., Kirkpatrick, D., Wright, R., Jones, E.W. Mol. Cell. Biol. (1991) [Pubmed]
  2. Vacuolar functions are involved in stress-protective effect of intracellular proline in Saccharomyces cerevisiae. Matsuura, K., Takagi, H. J. Biosci. Bioeng. (2005) [Pubmed]
  3. A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation. Robinson, J.S., Graham, T.R., Emr, S.D. Mol. Cell. Biol. (1991) [Pubmed]
  4. Saccharomyces cerevisiae mutants altered in vacuole function are defective in copper detoxification and iron-responsive gene transcription. Szczypka, M.S., Zhu, Z., Silar, P., Thiele, D.J. Yeast (1997) [Pubmed]
  5. A high dose of the STM1 gene suppresses the temperature sensitivity of the tom1 and htr1 mutants in Saccharomyces cerevisiae. Utsugi, T., Toh-e, A., Kikuchi, Y. Biochim. Biophys. Acta (1995) [Pubmed]
  6. A dual function for Deep orange in programmed autophagy in the Drosophila melanogaster fat body. Lindmo, K., Simonsen, A., Brech, A., Finley, K., Rusten, T.E., Stenmark, H. Exp. Cell Res. (2006) [Pubmed]
  7. Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae. Srivastava, A., Woolford, C.A., Jones, E.W. Genetics (2000) [Pubmed]
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