The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

URA4  -  dihydroorotase

Saccharomyces cerevisiae S288c

Synonyms: DHOase, Dihydroorotase, L9931.1, YLR420W
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of URA4


High impact information on URA4

  • The uninducible phenotype of this mutant is completely suppressed by an ochre suppressor, strengthening the hypothesis that PPR2 acts on URA4 transcription through the synthesis of a regulatory protein [3].
  • Dihydroorotase, the third enzymatic activity of the pyrimidine pathway, is encoded in Saccharomyces cerevisiae by a single gene URA4, which is induced at the transcriptional level by accumulation of ureidosuccinic acid [3].
  • They had also regained dihydroorotase activity, although its level did not correlate with the PYR3 gene copy number [1].
  • We also have genetically mapped the STE11 gene to chromosome XII, 40 centimorgans from ura4 [4].
  • The mammalian enzyme is part of a 240-kDa multifunctional protein which also has the second (aspartate carbamoyltransferase, EC, and third (dihydroorotase, EC activities of the pathway [5].

Biological context of URA4


Associations of URA4 with chemical compounds


Other interactions of URA4

  • Vector plasmids containing the URA3 gene and an autonomously replicating sequence (ARS) of S. cerevisiae produced sufficient amounts of Ura+ transformants from the ura4 mutant of S. exiguus [7].
  • Linkage to ura4 by about 44 centiMorgans places leu3 on the right arm of this chromosome [13].
  • Organization of the yeast URA2 gene: identification of a defective dihydroorotase-like domain in the multifunctional carbamoylphosphate synthetase-aspartate transcarbamylase complex [14].

Analytical, diagnostic and therapeutic context of URA4


  1. Cloning of the PYR3 gene of Ustilago maydis and its use in DNA transformation. Banks, G.R., Taylor, S.Y. Mol. Cell. Biol. (1988) [Pubmed]
  2. Structure of the Saccharomyces cerevisiae URA4 gene encoding dihydroorotase. Guyonvarch, A., Nguyen-Juilleret, M., Hubert, J.C., Lacroute, F. Mol. Gen. Genet. (1988) [Pubmed]
  3. Complete sequence of a eukaryotic regulatory gene. Hubert, J.C., Guyonvarch, A., Kammerer, B., Exinger, F., Liljelund, P., Lacroute, F. EMBO J. (1983) [Pubmed]
  4. Molecular cloning and characterization of the STE7 and STE11 genes of Saccharomyces cerevisiae. Chaleff, D.T., Tatchell, K. Mol. Cell. Biol. (1985) [Pubmed]
  5. Mammalian carbamyl phosphate synthetase (CPS). DNA sequence and evolution of the CPS domain of the Syrian hamster multifunctional protein CAD. Simmer, J.P., Kelly, R.E., Rinker, A.G., Scully, J.L., Evans, D.R. J. Biol. Chem. (1990) [Pubmed]
  6. Molecular cloning and DNA analysis of the orotidine-5'-phosphate decarboxylase gene from the yeast Saccharomyces exiguus Yp74L-3. Hisatomi, T., Kodama, T., Toba, H., Moto-oka, M., Watanabe, A., Tsuboi, M. Curr. Microbiol. (2000) [Pubmed]
  7. Identification of the orotidine-5'-phosphate decarboxylase gene and development of a transformation system in the yeast Saccharomyces exiguus Yp74L-3. Hisatomi, T., Kuroyanagi, S., Tsuboi, M. Biosci. Biotechnol. Biochem. (1998) [Pubmed]
  8. Pyrimidine biosynthesis in Saccharomyces cerevisiae: the ura2 cluster gene, its multifunctional enzyme product, and other structural or regulatory genes involved in de novo UMP synthesis. Denis-Duphil, M. Biochem. Cell Biol. (1989) [Pubmed]
  9. Drosophila RNA polymerase II elongation factor DmS-II has homology to mouse S-II and sequence similarity to yeast PPR2. Marshall, T.K., Guo, H., Price, D.H. Nucleic Acids Res. (1990) [Pubmed]
  10. As in Saccharomyces cerevisiae, aspartate transcarbamoylase is assembled on a multifunctional protein including a dihydroorotase-like cryptic domain in Schizosaccharomyces pombe. Lollier, M., Jaquet, L., Nedeva, T., Lacroute, F., Potier, S., Souciet, J.L. Curr. Genet. (1995) [Pubmed]
  11. Delta sequence of Ty1 transposon can initiate transcription of the distal part of the URA2 gene complex in Saccharomyces cerevisiae. Roelants, F., Potier, S., Souciet, J.L., de Montigny, J. FEMS Microbiol. Lett. (1997) [Pubmed]
  12. The rudimentary locus of Drosophila melanogaster. Segraves, W.A., Louis, C., Tsubota, S., Schedl, P., Rawls, J.M., Jarry, B.P. J. Mol. Biol. (1984) [Pubmed]
  13. Cloning, disruption and chromosomal mapping of yeast LEU3, a putative regulatory gene. Brisco, P.R., Cunningham, T.S., Kohlhaw, G.B. Genetics (1987) [Pubmed]
  14. Organization of the yeast URA2 gene: identification of a defective dihydroorotase-like domain in the multifunctional carbamoylphosphate synthetase-aspartate transcarbamylase complex. Souciet, J.L., Nagy, M., Le Gouar, M., Lacroute, F., Potier, S. Gene (1989) [Pubmed]
  15. Cloning and expression of the OMP decarboxylase gene URA4 from Schizosaccharomyces pombe. Bach, M.L. Curr. Genet. (1987) [Pubmed]
WikiGenes - Universities