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Gene Review

RDH54  -  DNA-dependent ATPase RDH54

Saccharomyces cerevisiae S288c

Synonyms: DNA repair and recombination protein RDH54, RAD homolog 54, Recombination factor TID1, TID1, Two hybrid interaction with DMC1 protein 1, ...
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Disease relevance of RDH54

  • Here we describe an E. coli expression system and purification scheme that together provide milligram quantities of nearly homogeneous Rdh54 [1].

High impact information on RDH54

  • Efficient D-loop formation occurs with even topologically relaxed DNA, suggesting that via specific protein-protein interactions, the negative supercoils produced by Rdh54 are used by Rad51 for making DNA joints [2].
  • We propose that Tid1p remodels Mcd1p-mediated cohesion early in meiotic prophase to facilitate interhomologue recombination and the subsequent segregation of homologous chromosomes [3].
  • Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae [3].
  • Sister chromatid-based DNA repair is mediated by RAD54, not by DMC1 or TID1 [4].
  • A rad54 mutation, in contrast, has relatively little effect on RecA homolog foci except when strains also contain a tid1/rdh54 mutation [5].

Biological context of RDH54


Associations of RDH54 with chemical compounds


Physical interactions of RDH54

  • Importantly, Rdh54 physically interacts with the Rad51 recombinase and promotes D-loop formation by the latter [1].

Regulatory relationships of RDH54


Other interactions of RDH54


Analytical, diagnostic and therapeutic context of RDH54

  • The Rdh54 expression and purification procedures described here should facilitate the functional dissection of this DNA recombination/repair factor [1].


  1. Yeast recombination factor Rdh54 functionally interacts with the Rad51 recombinase and catalyzes Rad51 removal from DNA. Chi, P., Kwon, Y., Seong, C., Epshtein, A., Lam, I., Sung, P., Klein, H.L. J. Biol. Chem. (2006) [Pubmed]
  2. Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1. Petukhova, G., Sung, P., Klein, H. Genes Dev. (2000) [Pubmed]
  3. Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae. Kateneva, A.V., Konovchenko, A.A., Guacci, V., Dresser, M.E. J. Cell Biol. (2005) [Pubmed]
  4. Sister chromatid-based DNA repair is mediated by RAD54, not by DMC1 or TID1. Arbel, A., Zenvirth, D., Simchen, G. EMBO J. (1999) [Pubmed]
  5. Tid1/Rdh54 promotes colocalization of rad51 and dmc1 during meiotic recombination. Shinohara, M., Gasior, S.L., Bishop, D.K., Shinohara, A. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  6. Characterization of the roles of the Saccharomyces cerevisiae RAD54 gene and a homologue of RAD54, RDH54/TID1, in mitosis and meiosis. Shinohara, M., Shita-Yamaguchi, E., Buerstedde, J.M., Shinagawa, H., Ogawa, H., Shinohara, A. Genetics (1997) [Pubmed]
  7. Schizosaccharomyces pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks. Catlett, M.G., Forsburg, S.L. Mol. Biol. Cell (2003) [Pubmed]
  8. RDH54, a RAD54 homologue in Saccharomyces cerevisiae, is required for mitotic diploid-specific recombination and repair and for meiosis. Klein, H.L. Genetics (1997) [Pubmed]
  9. Crossover interference in Saccharomyces cerevisiae requires a TID1/RDH54- and DMC1-dependent pathway. Shinohara, M., Sakai, K., Shinohara, A., Bishop, D.K. Genetics (2003) [Pubmed]
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