Disease relevance of RAD54B

• Homozygous mutations at highly conserved positions of RAD54B were observed in human primary lymphoma and colon cancer [1].
• RAD54B maps to human chromosome 8q21.3-q22 in a region associated with cancer-related chromosomal abnormalities [1].
• Foetal steroid binding protein (FSBP) which is present in normal human serum sometimes exhibits unusual sex-steroid binding; its effects on steroid action are uncertain but potentially important in view of the different levels seen in populations at different risk of breast cancer [2].

High impact information on RAD54B

• Inactivation of RAD54B in a colon cancer cell line resulted in severe reduction of targeted integration frequency [3].
• Sensitivity to DNA-damaging agents and sister-chromatid exchange were not affected in RAD54B-deficient cells [3].
• We verify the WRN-RAD51 and WRN-RAD54B direct interaction in vitro [4].
• Mutations of a novel human RAD54 homologue, RAD54B, in primary cancer [1].
• These findings suggest that RAD54B may play an active role in recombination processes in concert with other members of the RAD52 epistasis group [1].

Biological context of RAD54B

• Hemostasis was achieved by 4 minutes in 64 subjects (63%) in the FSBP group and 40 subjects (40%) in the TSG group (P =.0018) [5].
• The binding characteristics and quantitation of the recently reported fetal steroid binding protein (FSBP) cannot be determined on unpurified samples; an immunoassay was therefore desirable [6].
• Percentage finger systolic blood pressure (%FSBP) in response to finger cooling is used to assess vascular components of the hand-arm vibration syndrome and the measurement method is under discussion for standardization [7].
• METHOD: Thermal thresholds, vibrotactile thresholds, the finger skin temperature (FST) response to cold provocation and percentage finger systolic blood pressures (%FSBP) were measured by standardised procedures [8].
• After partial purification of FSBP in these samples, the protein exhibited saturable binding kinetics, median binding 25 (interquartiles 23-34) and 19 (13-25) nmol DHT/l in Japanese and British women, respectively (P less than 0.05) [9].

Anatomical context of RAD54B

• The expression of RAD54B was high in testis and spleen, which are active in meiotic and mitotic recombination [1].
• Patients were randomized to FSBP application, which requires a 3-minute period of arterial clamping to enable the fibrin clot to adhere, or to TSG application, which requires pressure from gauze sponges, after completion of the femoral artery anastomosis [5].
• Therefore, room temperature is expected to be controlled when assessing peripheral vascular components of the upper extremities using %FSBP response to finger cooling [7].

Associations of RAD54B with chemical compounds

• Steroid binding by FSBP in vivo may be determined predominantly by its interactions with other binding proteins [2].
• Studies of the binding of 5 alpha-dihydrotestosterone to FSBP were conducted at varying concentrations of human serum albumin (HSA) and sex hormone binding globulin (SHBG) [2].

Analytical, diagnostic and therapeutic context of RAD54B

• Silencing of the SNF2/SWI2 family members RAD54L or RAD54B, which are important for HR, reduced the rate of stable rAAV gene targeting approximately 5-fold [10].
• There was no significant difference in ICU stay, but hospital length of stay was shorter in the FSBP group compared with the TSG group, and the difference approached significance (median, 6.5 days; 95% CI, 5.00-7.00 days vs median, 7.0 days; 95% CI,. 6.00-8.00 days; P =.0565) [5].
• Use of the enzyme-linked immunosorbent assay confirmed the identity of FSBP separate from sex hormone binding globulin [6].

References