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Gene Review

CDC43  -  protein geranylgeranyltransferase type I...

Saccharomyces cerevisiae S288c

Synonyms: CAL1, Cell division cycle protein 43, G1864, GGTase-I-beta, Geranylgeranyl transferase type I subunit beta, ...
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Disease relevance of CDC43


High impact information on CDC43

  • Multiple copies of the CDC43 gene can partially suppress the growth and (1-3) beta-D-glucan synthase defect of the cwg2-1 mutant at the restrictive temperature [2].
  • The functional homology of Cwg2 with Cdc43, which has been implicated in the control of cell polarity, suggests a link between two morphogenetic events such as establishment of cell polarity and cell wall biosynthesis [2].
  • CDC42 maps distal to the rDNA on chromosome XII and CDC43 maps near lys5 on chromosome VII [3].
  • We report here that temperature-sensitive mutants defective in genes CDC42 and CDC43, like cdc24 mutants, fail to bud but continue growth at restrictive temperature, and thus arrest as large unbudded cells [3].
  • This mutant gene also suppressed a cal1 disruption even on a low-copy plasmid, suggesting that the product (designated S159N) can substitute for PGGT-I beta subunit in vivo [4].

Biological context of CDC43

  • The lethality of an S. cerevisiae cdc43 mutant can be suppressed by simultaneous overexpression of RHO1 and CDC42 on high-copy-number plasmids (Y. Ohya et al., Mol. Biol. Cell 4:1017, 1991; C. A. Trueblood, Y. Ohya, and J. Rine, Mol. Cell. Biol. 13:4260, 1993) [5].
  • CDC55 maps between CDC43 and CHC1 on the left arm of chromosome VII [6].
  • Nonetheless, all of the cal1/cdc43 mutants show temperature-sensitive growth phenotypes [7].
  • Thus, several phenotypic differences are observed among the cal1/cdc43 mutations, possibly due to alterations in substrate specificity caused by the mutations [7].
  • Amino acid sequence comparison suggests that the protein encoded by the Saccharomyces cerevisiae gene CDC43 is the yeast counterpart of the mammalian GGTase-I beta subunit [8].

Associations of CDC43 with chemical compounds

  • The putative CDC43 gene product contains a possible nuclear-localization signal sequence, a cysteine-rich domain and a histidine-rich domain, and a region that is similar in structure to alpha-helix-turn-alpha-helix structural domains present in some prokaryotic and eukaryotic DNA-binding proteins [9].
  • Here, we report the high yield bacterial expression of Ca GGTase I by coexpression of maltose binding protein fusion proteins of both the alpha (Ram2p) and beta (Cdc43p) subunits [10].
  • Limonene and perillyl alcohol and their major in vivo metabolite, perillic acid, are weak inhibitors of both mammalian and yeast protein farnesyl transferase (PFT) and protein geranylgeranyl transferase (PGGT) [11].

Other interactions of CDC43

  • The alpha and beta subunits of GGTase I from Saccharomyces cerevisiae are encoded by RAM2 and CDC43, respectively, and each is essential for viability [5].
  • The deletion analysis showed that only DNA fragments with the CDC43 and HAC1 genes can partially complement the rec41-1 mutation [12].
  • Protein geranylgeranyltransferase of Saccharomyces cerevisiae is specific for Cys-Xaa-Xaa-Leu motif proteins and requires the CDC43 gene product but not the DPR1 gene product [13].

Analytical, diagnostic and therapeutic context of CDC43


  1. CDC43 and RAM2 encode the polypeptide subunits of a yeast type I protein geranylgeranyltransferase. Mayer, M.L., Caplin, B.E., Marshall, M.S. J. Biol. Chem. (1992) [Pubmed]
  2. The Schizosaccharomyces pombe cwg2+ gene codes for the beta subunit of a geranylgeranyltransferase type I required for beta-glucan synthesis. Díaz, M., Sanchez, Y., Bennett, T., Sun, C.R., Godoy, C., Tamanoi, F., Duran, A., Perez, P. EMBO J. (1993) [Pubmed]
  3. CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae. Adams, A.E., Johnson, D.I., Longnecker, R.M., Sloat, B.F., Pringle, J.R. J. Cell Biol. (1990) [Pubmed]
  4. Mutant farnesyltransferase beta subunit of Saccharomyces cerevisiae that can substitute for geranylgeranyltransferase type I beta subunit. Mitsuzawa, H., Esson, K., Tamanoi, F. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  5. Geranylgeranyltransferase I of Candida albicans: null mutants or enzyme inhibitors produce unexpected phenotypes. Kelly, R., Card, D., Register, E., Mazur, P., Kelly, T., Tanaka, K.I., Onishi, J., Williamson, J.M., Fan, H., Satoh, T., Kurtz, M. J. Bacteriol. (2000) [Pubmed]
  6. CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase. Healy, A.M., Zolnierowicz, S., Stapleton, A.E., Goebl, M., DePaoli-Roach, A.A., Pringle, J.R. Mol. Cell. Biol. (1991) [Pubmed]
  7. Mutational analysis of the beta-subunit of yeast geranylgeranyl transferase I. Ohya, Y., Caplin, B.E., Qadota, H., Tibbetts, M.F., Anraku, Y., Pringle, J.R., Marshall, M.S. Mol. Gen. Genet. (1996) [Pubmed]
  8. cDNA cloning and expression of rat and human protein geranylgeranyltransferase type-I. Zhang, F.L., Diehl, R.E., Kohl, N.E., Gibbs, J.B., Giros, B., Casey, P.J., Omer, C.A. J. Biol. Chem. (1994) [Pubmed]
  9. Isolation and sequence analysis of CDC43, a gene involved in the control of cell polarity in Saccharomyces cerevisiae. Johnson, D.I., O'Brien, J.M., Jacobs, C.W. Gene (1990) [Pubmed]
  10. Expression and characterization of protein geranylgeranyltransferase type I from the pathogenic yeast Candida albicans and identification of yeast selective enzyme inhibitors. Smalera, I., Williamson, J.M., Baginsky, W., Leiting, B., Mazur, P. Biochim. Biophys. Acta (2000) [Pubmed]
  11. The inhibition of protein prenyltransferases by oxygenated metabolites of limonene and perillyl alcohol. Gelb, M.H., Tamanoi, F., Yokoyama, K., Ghomashchi, F., Esson, K., Gould, M.N. Cancer Lett. (1995) [Pubmed]
  12. The REC41 gene of Saccharomyces cerevisiae: isolation and genetic analysis. Chepurnaya, O.V., Kozhina, T.N., Peshekhonov, V.T., Korolev, V.G. Mutat. Res. (2001) [Pubmed]
  13. Protein geranylgeranyltransferase of Saccharomyces cerevisiae is specific for Cys-Xaa-Xaa-Leu motif proteins and requires the CDC43 gene product but not the DPR1 gene product. Finegold, A.A., Johnson, D.I., Farnsworth, C.C., Gelb, M.H., Judd, S.R., Glomset, J.A., Tamanoi, F. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  14. Isolation and sequence analysis of CDC43, a gene involved in the control of cell polarity in Saccharomyces cerevisiae. Johnson, D.I., O'Brien, J.M., Jacobs, C.W. Gene (1991) [Pubmed]
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