Disease relevance of SPR3

• Similarly, the levels of beta-galactosidase present in sporulating cells harboring fusions between SPR3 and Escherichia coli lacZ increased at least 700-fold [1].

High impact information on SPR3

• Deletion of SPR3 does not prevent the localization of Cdc3p and Cdc11p, but these proteins appear to be less well organized, and the intensity of their staining is reduced [2].
• In one genetic background, deletion of SPR3 produced a threefold reduction in sporulation efficiency, although meiosis appeared to be completed normally [2].
• The predicted SPR3 product shows 25-40% identity in amino acid sequence to the previously known septins from S. cerevisiae and other organisms [2].
• The SPR3 gene encodes a sporulation-specific homolog of the yeast Cdc3/10/11/12 family of bud neck filament proteins [3].
• A palindromic sequence located near the TATA box is essential to the developmental regulation of this gene and is the only element directly activating SPR3 at the right time during sporulation [3].

Biological context of SPR3

• Immunofluorescence observations suggest that Spr3p, Cdc3p, and Cdc11p are localized to the leading edges of the membrane sacs that form near the spindle-pole bodies and gradually extend to engulf the nuclear lobes that contain the haploid chromosome sets, thus forming the spores [2].
• Regulation of gene expression during meiosis in Saccharomyces cerevisiae: SPR3 is controlled by both ABFI and a new sporulation control element [3].
• The SPR3 gene is selectively activated only during the sporulation phase of the Saccharomyces cerevisiae (Sc) life cycle [4].
• The sequence immediately upstream from the tsp contains consensus binding sites for the HAP2/3/4 and ABFI transcription factors, a T-rich sequence and two putative novel elements for mid to late sporulation, termed SPR3 and PAL [4].

Anatomical context of SPR3

• The SPR3 gene encodes a sporulation-specific homologue of the yeast CDC3/10/11/12 family of bud neck microfilaments and is regulated by ABFI [4].

Other interactions of SPR3

• We conclude that SPR1, SPR2 and SPR3 transcription is modulated during sporulation, possibly in response to earlier events in the process [1].
• Deletion of SPR28 in either a wild-type or an spr3 delta background produced no obvious abnormalities in vegetative cells and had little or no effect on sporulation, suggesting that the septins have redundant roles during spore formation [5].

Analytical, diagnostic and therapeutic context of SPR3

• Electrophoretic mobility shift assay (EMSA) and footprint analyses demonstrated that the ABFI protein binds to a region containing the putative ABFI site in vitro, and site-directed mutagenesis showed that the ABFI motif is essential for expression of SPR3 at the appropriate stage in sporulating cells [4].

References