High impact information on CDC3

• We have identified four major SUMO attachment-site lysine residues in Cdc3, one in Cdc11, and two in Shs1, all within the consensus sequence (IVL)KX(ED) [1].
• We also used the CDC3-specific and CDC12-specific antibodies to investigate the timing of localization of these proteins to the budding site [2].
• The results suggest that the CDC3 protein is organized into a ring at the budding site well before bud emergence and remains so organized for some time after cytokinesis [2].
• Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC3 gene product and the timing of events at the budding site [2].
• The coiled-coil domain is important for interaction with Cdc3 and Bem4 [3].

Biological context of CDC3

• Mapping of the Saccharomyces cerevisiae CDC3, CDC25, and CDC42 genes to chromosome XII by chromosome blotting and tetrad analysis [4].
• Northern (RNA) studies using the AAH-, APRT-, and CDC3-coding regions indicated that AAH regulation was not mediated at the level of transcription or mRNA degradation [5].
• To ascertain whether this highly coordinated system could function independently of other cell cycle events, we reexamined the septum-like structures made by the septin mutant cdc3 at various sites on the cell cortex at the nonpermissive temperature [6].
• Cell wall structures that partition membrane-bound portions of cytoplasm were formed at sites along the peripheral wall when a cytokinesis-defective cell division cycle mutant (cdc3) of Saccharomyces cerevisiae was grown at a restrictive temperature [7].
• Analysis of a mutant allele that cannot be phosphorylated at these sites revealed an effect of Cdc28 phosphorylation of Cdc3 at the time of budding [8].

Anatomical context of CDC3

• The SPR3 gene encodes a sporulation-specific homologue of the yeast CDC3/10/11/12 family of bud neck microfilaments and is regulated by ABFI [9].
• Examination of mating cells and cells responding to purified mating pheromone revealed novel arrangements of the CDC3 and CDC12 products in the regions of cell wall reorganization [2].
• Under conditions that induce hyphae, both Cdc3p and Int1p localized to a ring distal to the junction of the mother cell and germ tube [10].

Physical interactions of CDC3

• Cdc10 interacts with a Cdc3-Cdc12 complex independently of the CTE of either protein [11].

Enzymatic interactions of CDC3

• Two serines near the C-terminus of Cdc3 are phosphorylated in a Cdc28-dependent manner [8].

Co-localisations of CDC3

• In C. albicans, Int1p was important for the axial budding pattern and colocalized with Cdc3p septin in a ring at the mother-bud neck of yeast and pseudohyphal cells [10].

Regulatory relationships of CDC3

• Nfi1/Siz2 stimulated Smt3 conjugation to Cdc3 in vitro [12].

Other interactions of CDC3

• We showed previously that the CDC3 and CDC12 gene products localize to the region of the mother-bud neck and are probably constituents of the ring of 10 nm filaments [13].
• We have isolated and characterized C. albicans homologs of the S. cerevisiae CDC3 and CDC10 genes [14].
• Thus, we conclude that Smt3 was conjugated to Cdc3 in septin rings localized at the mother-bud neck [15].
• With the fluorescent fusion proteins mentioned above, we observed that in cdc3 at 37 degrees C both Myo1p and Chs2p colocalize at different spots of the cell cortex [6].
• Using this polysumoylation-deficient mutant Smt3, we found that Cdc3 and Nfi1/Siz2 were modified with Smt3 at multiple sites [12].

Analytical, diagnostic and therapeutic context of CDC3

• In immunofluorescence experiments, these antibodies decorated the neck regions of wild-type and mutant cells in patterns suggesting that the CDC3 gene product is also a constituent of the ring of 10-nm filaments [2].
• Homologs of the yeast neck filament associated genes: isolation and sequence analysis of Candida albicans CDC3 and CDC10 [14].
• Indeed immunoprecipitation experiments revealed that Cdc3, one of septin components, was modified with Smt3 [15].

References