High impact information on ARP4

• The purified Esa1-Arp4 HAT complex acetylates linear nucleosomal arrays with far greater efficiency than circular arrays in vitro, indicating that it preferentially acetylates nucleosomes near a break site [1].
• The act3 his4delta-ADE2 colonies display both white and red sectors, showing that the two different phenotypes are possible in a single colony [2].
• According to sequence comparison, the basal core structure of conventional actin may well be conserved in Act3 [3].
• The deduced amino acid sequence of Act3 is less related to conventional actins than is the deduced amino acid sequence of Act2, mainly because of three unique hydrophilic [corrected] segments [3].
• BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere [4].

Biological context of ARP4

• ARP4, an essential gene of Saccharomyces cerevisiae, codes for a nuclear actin-related protein [5].
• Furthermore, the use of reporter genes confirmed that Arp4p could be involved in some general mechanism of transcriptional regulation [6].
• The same effect was observed upon treatment with rapamycin, indicating an unexpected relationship of Arp4 to TOR-mediated cell cycle arrest [5].
• The roles of the core actin structure (the ATP-binding pocket) and a putative Nuclear Localization Signal (NLS) of Arp4p were tested by targeted mutagenesis [6].
• We used a mutant strain with a single amino acid substitution (G161D) in the conserved actin fold domain to investigate the influence of Arp4 on stress and nitrogen catabolite repression genes [5].

Associations of ARP4 with chemical compounds

• Both ts mutants were formamide-sensitive which supports a structural relatedness of Act3p/Arp4p to actin; they were also hypersensitive against hydroxyurea and ultraviolet irradiation pointing to a possible role of Act3p/Arp4p in DNA replication and repair [7].
• We observed that mutations in the putative ATP-binding pocket of Act3p/Arp4p increased its concentration in the high molecular mass complexes and, conversely, that an excess of ATP or ATPgammaS led to the release of wild-type Act3p/Arp4p from the complexes [8].
• Immunochemical study on the mannans of Candida albicans NIH A-207, NIH B-792, and J-1012 strains prepared by fractional precipitation with cetyltrimethylammonium bromide [9].

Other interactions of ARP4

• We have ordered the new ARPs relative to this group of actins and propose to name the more distantly related ARP members, according to their amino acid identity and similarity, Arp4-Arp10 [10].

Analytical, diagnostic and therapeutic context of ARP4

• Chromatin immunoprecipitation assays show that Act3p/Arp4 is bound to the entire his4-912delta promoter region [11].

References