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Gene Review:

REV7 - Rev7p

Saccharomyces cerevisiae S288c

Synonyms: DNA polymerase zeta processivity subunit, Revertibility protein 7, YIL139C

Sabbioneda, S. et al., Okada, T. et al., Murakumo, Y., Lawrence, C.W. et al., Sakai, W. et al., et al.

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Disease relevance of REV7

REV1-/- REV3-/- REV7-/- cells showed spontaneous cell death, chromosomal instability/fragility, and hypersensitivity to various genotoxic treatments as observed in each of the single mutants [1].

High impact information on REV7

Mutagenesis induced by DNA damage in Saccharomyces cerevisiae requires the products of the REV1, REV3 and REV7 genes [2].
Here, we disrupted Rev1, Rev3, and Rev7 in the chicken B-lymphocyte line DT40 [1].
Polzeta consists of two subunits, one encoded by REV3 (the catalytic subunit) and the other encoded by REV7 [3].
In this work, we demonstrate both an in vivo and in vitro physical interaction between the Mec3 and Ddc1 subunits of the 9-1-1 clamp and the Rev7 subunit of the Polzeta TLS polymerase [4].
The human homologs of S. cerevisiae REV1, REV3, and REV7 were identified, and it is revealed that the human REV proteins have similar functions to their yeast counterparts, however, a large part of the mechanisms of mutagenesis employing REV proteins are still unclear [5].

Biological context of REV7

Alteration of ultraviolet-induced mutagenesis in yeast through molecular modulation of the REV3 and REV7 gene expression [6].
The REV7 gene is located about 12 cM distal to HIS5 on chromosome IX [7].
To help understand the mechanisms of mutagenic DNA repair in the N. crassa more extensively, we identified N. crassa homologs of yeast REV1 and REV7 and obtained mutants ncrev1 or ncrev7, which had similar phenotypes to the upr-1 mutant [8].
To address this possibility, Saccharomyces cerevisiae strains lacking either pol zeta (rev7), pol eta (rad30), or both were tested for trinucleotide repeat (TNR) contractions using three separate, sensitive genetic assays [9].

Associations of REV7 with chemical compounds

Three allelic mutations of a new yeast gene, which we have named REV7, have been isolated by testing 313 methyl methane sulfonate sensitive mutants for UV-induced reversion of a lys2 allele [7].
Like rev3 and rev7 mutations, ngm2-1 also has little influence on the reversion of the proline missense allele, cyc1-115 [10].

Physical interactions of REV7

Importantly, the polymerase-associated domain (PAD) of Rev1 mediates its binding to Rev7 [11].

References

Multiple roles of vertebrate REV genes in DNA repair and recombination. Okada, T., Sonoda, E., Yoshimura, M., Kawano, Y., Saya, H., Kohzaki, M., Takeda, S. Mol. Cell. Biol. (2005) [Pubmed]
Deoxycytidyl transferase activity of yeast REV1 protein. Nelson, J.R., Lawrence, C.W., Hinkle, D.C. Nature (1996) [Pubmed]
ATR homolog Mec1 controls association of DNA polymerase zeta-Rev1 complex with regions near a double-strand break. Hirano, Y., Sugimoto, K. Curr. Biol. (2006) [Pubmed]
The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae. Sabbioneda, S., Minesinger, B.K., Giannattasio, M., Plevani, P., Muzi-Falconi, M., Jinks-Robertson, S. J. Biol. Chem. (2005) [Pubmed]
The property of DNA polymerase zeta: REV7 is a putative protein involved in translesion DNA synthesis and cell cycle control. Murakumo, Y. Mutat. Res. (2002) [Pubmed]
Alteration of ultraviolet-induced mutagenesis in yeast through molecular modulation of the REV3 and REV7 gene expression. Rajpal, D.K., Wu, X., Wang, Z. Mutat. Res. (2000) [Pubmed]
REV7, a new gene concerned with UV mutagenesis in yeast. Lawrence, C.W., Das, G., Christensen, R.B. Mol. Gen. Genet. (1985) [Pubmed]
Isolation and genetic characterization of the Neurospora crassa REV1 and REV7 homologs: evidence for involvement in damage-induced mutagenesis. Sakai, W., Wada, Y., Naoi, Y., Ishii, C., Inoue, H. DNA Repair (Amst.) (2003) [Pubmed]
Examining the potential role of DNA polymerases eta and zeta in triplet repeat instability in yeast. Dixon, M.J., Lahue, R.S. DNA Repair (Amst.) (2002) [Pubmed]
The isolation and characterization of ngm2, a mutation that affects nitrosoguanidine mutagenesis in yeast. Nisson, P.E., Lawrence, C.W. Mol. Gen. Genet. (1986) [Pubmed]
Complex formation of yeast Rev1 and Rev7 proteins: a novel role for the polymerase-associated domain. Acharya, N., Haracska, L., Johnson, R.E., Unk, I., Prakash, S., Prakash, L. Mol. Cell. Biol. (2005) [Pubmed]

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