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Gene Review

MEC3  -  Mec3p

Saccharomyces cerevisiae S288c

Synonyms: DNA damage checkpoint control protein MEC3, L8003.15, PIP3, PSO9, YLR288C
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High impact information on MEC3

  • Finally, deletion of MEC3 results in telomere elongation, whereas cells with deletions of both SET1 and MEC3 do not have elongated telomeres [1].
  • An assay that measures DNA damage processing in vivo showed that the checkpoint genes RAD17, RAD24, and MEC3 activated an exonuclease that degrades DNA [2].
  • Loss of Stn1 function activated the RAD9 and MEC3 G2/M checkpoints, therefore confirming that DNA damage is generated [3].
  • A structural model of the UNC-86 POU domain, including base pairs and amino acid residues required for MEC-3 interaction, revealed that P145 and L195 are part of a hydrophobic pocket which is similar to the OCA-B-binding domain of the mammalian POU protein, Oct-1 [4].
  • We demonstrate that Ddc1p interacts physically in vivo with Mec3p, and this interaction requires Rad17p [5].

Biological context of MEC3


Anatomical context of MEC3


Associations of MEC3 with chemical compounds


Physical interactions of MEC3


Other interactions of MEC3

  • On the other hand, Rad24 is not found to associate with Rad17, Mec3, and Ddc1 [6].
  • A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1 [11].
  • The DDC1 gene was identified, together with MEC3 and other checkpoint genes, during a screening for mutations causing synthetic lethality when combined with a conditional allele altering DNA primase [12].
  • Its known interactions with the checkpoint proteins Mec3p and Ddc1p in a PCNA-like complex indicate a sensor role in damage recognition [13].
  • In this work, we demonstrate both an in vivo and in vitro physical interaction between the Mec3 and Ddc1 subunits of the 9-1-1 clamp and the Rev7 subunit of the Polzeta TLS polymerase [14].

Analytical, diagnostic and therapeutic context of MEC3


  1. Interaction between Set1p and checkpoint protein Mec3p in DNA repair and telomere functions. Corda, Y., Schramke, V., Longhese, M.P., Smokvina, T., Paciotti, V., Brevet, V., Gilson, E., Géli, V. Nat. Genet. (1999) [Pubmed]
  2. Yeast checkpoint genes in DNA damage processing: implications for repair and arrest. Lydall, D., Weinert, T. Science (1995) [Pubmed]
  3. Stn1, a new Saccharomyces cerevisiae protein, is implicated in telomere size regulation in association with Cdc13. Grandin, N., Reed, S.I., Charbonneau, M. Genes Dev. (1997) [Pubmed]
  4. Protein interaction surface of the POU transcription factor UNC-86 selectively used in touch neurons. Röhrig, S., Röckelein, I., Donhauser, R., Baumeister, R. EMBO J. (2000) [Pubmed]
  5. Mec1p is essential for phosphorylation of the yeast DNA damage checkpoint protein Ddc1p, which physically interacts with Mec3p. Paciotti, V., Lucchini, G., Plevani, P., Longhese, M.P. EMBO J. (1998) [Pubmed]
  6. Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway. Kondo, T., Matsumoto, K., Sugimoto, K. Mol. Cell. Biol. (1999) [Pubmed]
  7. G2/M checkpoint genes of Saccharomyces cerevisiae: further evidence for roles in DNA replication and/or repair. Lydall, D., Weinert, T. Mol. Gen. Genet. (1997) [Pubmed]
  8. Reconstitution of the mammalian PI3K/PTEN/Akt pathway in yeast. Rodríguez-Escudero, I., Roelants, F.M., Thorner, J., Nombela, C., Molina, M., Cid, V.J. Biochem. J. (2005) [Pubmed]
  9. Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primase. Longhese, M.P., Fraschini, R., Plevani, P., Lucchini, G. Mol. Cell. Biol. (1996) [Pubmed]
  10. Function of Rad17/Mec3/Ddc1 and its partial complexes in the DNA damage checkpoint. Majka, J., Burgers, P.M. DNA Repair (Amst.) (2005) [Pubmed]
  11. A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1. Giannattasio, M., Sommariva, E., Vercillo, R., Lippi-Boncambi, F., Liberi, G., Foiani, M., Plevani, P., Muzi-Falconi, M. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  12. The novel DNA damage checkpoint protein ddc1p is phosphorylated periodically during the cell cycle and in response to DNA damage in budding yeast. Longhese, M.P., Paciotti, V., Fraschini, R., Zaccarini, R., Plevani, P., Lucchini, G. EMBO J. (1997) [Pubmed]
  13. Characterization of DNA damage-stimulated self-interaction of Saccharomyces cerevisiae checkpoint protein Rad17p. Zhang, H., Zhu, Z., Vidanes, G., Mbangkollo, D., Liu, Y., Siede, W. J. Biol. Chem. (2001) [Pubmed]
  14. The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae. Sabbioneda, S., Minesinger, B.K., Giannattasio, M., Plevani, P., Muzi-Falconi, M., Jinks-Robertson, S. J. Biol. Chem. (2005) [Pubmed]
  15. Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3. Cardone, J.M., Revers, L.F., Machado, R.M., Bonatto, D., Brendel, M., Henriques, J.A. DNA Repair (Amst.) (2006) [Pubmed]
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