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Gene Review

MET30  -  Met30p

Saccharomyces cerevisiae S288c

Synonyms: E3 ubiquitin ligase complex SCF(Met30) subunit MET30, F-box protein MET30, Methionine-requiring protein 30, YIL046W
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High impact information on MET30


Biological context of MET30


Anatomical context of MET30

  • Mutations to MET30 cause defects in both the MAM as a donor of PtdSer, and the mitochondria as an acceptor of PtdSer in the transport reaction [6].
  • Reconstitution of different permutations of MAM and mitochondria from wild type and pstA1-1 strains demonstrates that the MET30 gene product affects both organelles [7].

Associations of MET30 with chemical compounds

  • These mutations identified a new gene, MET30, and were shown to also affect the regulation of the methyl cycle [8].
  • Furthermore, we provide evidence that Met30p abundance is regulated by the availability of L-methionine [4].
  • We identified one target of Met30p as Met4p, a transcriptional activator regulating the sulfate assimilation pathway [8].

Physical interactions of MET30


Regulatory relationships of MET30


Other interactions of MET30

  • Surprisingly, Met4 is a relatively stable protein and its abundance is not influenced by Met30 [1].
  • Increased levels of Clb5 could not, however, suppress the cell proliferation defect of met30 mutants [5].
  • Here, we review the substrates and pathways regulated by the yeast F-box proteins Cdc4, Grr1 and Met30 [9].
  • Sequence analysis reveals that Met30p contains five copies of the WD40 motif within its carboxy-terminal part, like the yeast transcriptional repressors Hir1p and Tup1p [8].


  1. Regulation of transcription by ubiquitination without proteolysis: Cdc34/SCF(Met30)-mediated inactivation of the transcription factor Met4. Kaiser, P., Flick, K., Wittenberg, C., Reed, S.I. Cell (2000) [Pubmed]
  2. Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1. Kaiser, P., Sia, R.A., Bardes, E.G., Lew, D.J., Reed, S.I. Genes Dev. (1998) [Pubmed]
  3. Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30 )complex. Rouillon, A., Barbey, R., Patton, E.E., Tyers, M., Thomas, D. EMBO J. (2000) [Pubmed]
  4. The abundance of Met30p limits SCF(Met30p) complex activity and is regulated by methionine availability. Smothers, D.B., Kozubowski, L., Dixon, C., Goebl, M.G., Mathias, N. Mol. Cell. Biol. (2000) [Pubmed]
  5. The F-box protein Met30 is required for multiple steps in the budding yeast cell cycle. Su, N.Y., Flick, K., Kaiser, P. Mol. Cell. Biol. (2005) [Pubmed]
  6. Protein and lipid motifs regulate phosphatidylserine traffic in yeast. Voelker, D.R. Biochem. Soc. Trans. (2005) [Pubmed]
  7. Phosphatidylserine transport to the mitochondria is regulated by ubiquitination. Schumacher, M.M., Choi, J.Y., Voelker, D.R. J. Biol. Chem. (2002) [Pubmed]
  8. Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats. Thomas, D., Kuras, L., Barbey, R., Cherest, H., Blaiseau, P.L., Surdin-Kerjan, Y. Mol. Cell. Biol. (1995) [Pubmed]
  9. SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis. Willems, A.R., Goh, T., Taylor, L., Chernushevich, I., Shevchenko, A., Tyers, M. Philos. Trans. R. Soc. Lond., B, Biol. Sci. (1999) [Pubmed]
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