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Gene Review

DAK1  -  Dak1p

Saccharomyces cerevisiae S288c

Synonyms: DHA kinase 1, Dihydroxyacetone kinase 1, Glycerone kinase 1, Triokinase 1, Triose kinase 1, ...
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Disease relevance of DAK1


High impact information on DAK1

  • The importance of DAK was clearly apparent for cells where both isogenes were deleted (dak1 Delta dak2 Delta), since this strain was highly sensitive to DHA [3].
  • We furthermore show that their substrate, dihydroxyacetone (DHA), is toxic to yeast cells and that the detoxification is dependent on functional DAK [3].
  • The sequence of another salt regulated protein resolved in the 2-D gel revealed identity to a bacterial dihydroxyacetone kinase, thus indicating salt induced glycerol dissimilation [4].
  • Of the seven proteins that were identified, two were previously not known to be under HOG pathway control: Ald6p, an isoform of aldehyde dehydrogenase and Dak1p, a putative dihydroxyacetone kinase [5].
  • As the amino acid sequence was most closely homologous (68% identity) to the S. cerevisiae Dak1p, we named the gene and protein ZrDAK1 and ZrDak1p, respectively [6].

Chemical compound and disease context of DAK1


Biological context of DAK1


Associations of DAK1 with chemical compounds

  • Furthermore, our results indicate that GCY1 and DAK1, tentatively encoding glycerol dehydrogenase and dihydroxyacetone kinase, respectively, may be involved in the redox regulation of S. cerevisiae [8].
  • To investigate Saccharomyces cerevisiae physiology during growth on the conditionally toxic triose dihydroxyacetone (DHA), protein expression was studied in strains overexpressing either of the two dihydroxyacetone kinase isogenes, DAK1 or DAK2, that grow well utilizing DHA as a carbon and energy source [9].
  • Proteins with increased expression during growth under sodium chloride stress could be grouped into three classes with respect to PKA activity, with the glycerol metabolic proteins GPD1, GPP2 and DAK1 standing out as independent of PKA [10].
  • Global protein expression in Saccharomyces cerevisiae strains either deleted for both yeast dihydroxyacetone kinases (DAK1 and DAK2) or overexpressing DAK1, was characterized by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) [11].
  • We have cloned the DAK gene from Pichia pastoris by functional complementation of a mutant that was unable to grow on methanol [7].

Other interactions of DAK1

  • The kinetic properties of the two isoforms were similar, exhibiting K(m)((DHA)) of 22 and 5 microm and K(m)((ATP)) of 0.5 and 0.1 mm for Dak1p and Dak2p, respectively [3].
  • The gene dak1 encoding a dihydroxyacetone kinase (DHAK) isoenzyme I, one of two isoenzymes in the Schizosaccharomyces pombe IFO 0354 strain, was cloned and sequenced [1].
  • In the N-terminal acetyltransferase mutant nat1delta, (M)A-Dak1p species were converted into the basic form, arguing twin spots to represent forms with acetylated and deacetylated N-termini [11].

Analytical, diagnostic and therapeutic context of DAK1


  1. Cloning and overexpression in Escherichia coli of the gene encoding dihydroxyacetone kinase isoenzyme I from Schizosaccharomyces pombe, and its application to dihydroxyacetone phosphate production. Itoh, N., Tujibata, Y., Liu, J.Q. Appl. Microbiol. Biotechnol. (1999) [Pubmed]
  2. Dihydroxyacetone synthase from a methanol-utilizing carboxydobacterium, Acinetobacter sp. strain JC1 DSM 3803. Ro, Y.T., Eom, C.Y., Song, T., Cho, J.W., Kim, Y.M. J. Bacteriol. (1997) [Pubmed]
  3. Dihydroxyacetone kinases in Saccharomyces cerevisiae are involved in detoxification of dihydroxyacetone. Molin, M., Norbeck, J., Blomberg, A. J. Biol. Chem. (2003) [Pubmed]
  4. Osmoresponsive proteins and functional assessment strategies in Saccharomyces cerevisiae. Blomberg, A. Electrophoresis (1997) [Pubmed]
  5. Osmoregulation and protein expression in a pbs2delta mutant of Saccharomyces cerevisiae during adaptation to hypersaline stress. Akhtar, N., Blomberg, A., Adler, L. FEBS Lett. (1997) [Pubmed]
  6. Cloning, sequencing and characterization of a gene encoding dihydroxyacetone kinase from Zygosaccharomyces rouxii NRRL2547. Wang, Z.X., Kayingo, G., Blomberg, A., Prior, B.A. Yeast (2002) [Pubmed]
  7. The Pichia pastoris dihydroxyacetone kinase is a PTS1-containing, but cytosolic, protein that is essential for growth on methanol. Lüers, G.H., Advani, R., Wenzel, T., Subramani, S. Yeast (1998) [Pubmed]
  8. Microaerobic glycerol formation in Saccharomyces cerevisiae. Costenoble, R., Valadi, H., Gustafsson, L., Niklasson, C., Franzén, C.J. Yeast (2000) [Pubmed]
  9. Dihydroxyacetone detoxification in Saccharomyces cerevisiae involves formaldehyde dissimilation. Molin, M., Blomberg, A. Mol. Microbiol. (2006) [Pubmed]
  10. The level of cAMP-dependent protein kinase A activity strongly affects osmotolerance and osmo-instigated gene expression changes in Saccharomyces cerevisiae. Norbeck, J., Blomberg, A. Yeast (2000) [Pubmed]
  11. Fragmentation of dihydroxyacetone kinase 1 from Saccharomyces cerevisiae indicates a two-domain structure. Molin, M., Larsson, T., Karlsson, K.A., Blomberg, A. Proteomics (2003) [Pubmed]
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