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Gene Review

SEC8  -  Sec8p

Saccharomyces cerevisiae S288c

Synonyms: Exocyst complex component SEC8, YP9499.11, YPR055W
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High impact information on SEC8


Biological context of SEC8

  • The SEC8 and SEC15 genes are essential for exocytosis in the yeast Saccharomyces cerevisiae and exhibit strong genetic interactions with SEC4, a gene of the ras superfamily [4].
  • When single exocytotic events were imaged by total internal reflection fluorescence microscopy, the fluorescence of the insulin surrogate, neuropeptide Y-monomeric red fluorescent protein brightened, diffused, and then vanished with kinetics that were unaffected by overexpression of truncated SEC8 or SEC10 [5].

Anatomical context of SEC8

  • Two of these genes, SEC8 and SEC15, encode components of a 1-2-million D multi-subunit complex that is found in the cytoplasm and associated with the plasma membrane [3].
  • Seb1p was coimmunoprecipitated from yeast cell lysates with Sec15p and Sec8p, components of the exocyst complex, and with Sec4p, a secretory vesicle associated Rab GTPase that binds to Sec15p and is essential for exocytosis [6].
  • A novel 17S complex composed of eight proteins including rsec6 and rsec8, the rat homologues of the yeast secretory proteins, Sec6p and Sec8p, has been identified in rat brain cytosol [7].

Associations of SEC8 with chemical compounds

  • Here, we examined the role of the SEC6-SEC8 (exocyst) complex, implicated in trafficking of secretory vesicles to fusion sites in the plasma membrane in yeast and in regulating glucose-stimulated insulin secretion from pancreatic MIN6 beta cells [5].
  • The Sec8p sequence contains a 202 amino acid region that is 25% identical to the leucine rich domain of yeast adenylate cyclase that has been implicated in ras responsiveness [4].

Physical interactions of SEC8


Other interactions of SEC8

  • Human Sec3 (hSec3) shares 17% sequence identity with yeast Sec3p, interacts in the two-hybrid system with other subunits of the complex (Sec5 and Sec8), and is expressed in almost all tissues tested [8].

Analytical, diagnostic and therapeutic context of SEC8


  1. Sec3p is a spatial landmark for polarized secretion in budding yeast. Finger, F.P., Hughes, T.E., Novick, P. Cell (1998) [Pubmed]
  2. The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. TerBush, D.R., Maurice, T., Roth, D., Novick, P. EMBO J. (1996) [Pubmed]
  3. Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae. TerBush, D.R., Novick, P. J. Cell Biol. (1995) [Pubmed]
  4. Sec8p and Sec15p are components of a plasma membrane-associated 19.5S particle that may function downstream of Sec4p to control exocytosis. Bowser, R., Müller, H., Govindan, B., Novick, P. J. Cell Biol. (1992) [Pubmed]
  5. Mammalian exocyst complex is required for the docking step of insulin vesicle exocytosis. Tsuboi, T., Ravier, M.A., Xie, H., Ewart, M.A., Gould, G.W., Baldwin, S.A., Rutter, G.A. J. Biol. Chem. (2005) [Pubmed]
  6. The beta subunit of the Sec61p endoplasmic reticulum translocon interacts with the exocyst complex in Saccharomyces cerevisiae. Toikkanen, J.H., Miller, K.J., Söderlund, H., Jäntti, J., Keränen, S. J. Biol. Chem. (2003) [Pubmed]
  7. Characterization of a cDNA encoding a subunit of the rat brain rsec6/8 complex. Hazuka, C.D., Hsu, S.C., Scheller, R.H. Gene (1997) [Pubmed]
  8. The Sec6/8 complex in mammalian cells: characterization of mammalian Sec3, subunit interactions, and expression of subunits in polarized cells. Matern, H.T., Yeaman, C., Nelson, W.J., Scheller, R.H. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
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