The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

PZF1  -  Pzf1p

Saccharomyces cerevisiae S288c

Synonyms: P9677.9, TFC2, TFIIIA, Transcription factor IIIA, YPR186C
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of PZF1


High impact information on PZF1


Biological context of PZF1

  • Analysis of DNA upstream of the RPB6 gene revealed an open reading frame that predicts a protein, designated PZF1, with nine C2H2 zinc fingers [6].
  • We found that purified recombinant PZF1 specifically binds the internal control region (ICR) of the 5S rRNA gene in S. cerevisiae [6].
  • The PZF1 gene was found to be single copy, transcribed into a 1.5-kilobase mRNA, and essential for yeast cell viability [6].
  • Interestingly, the yeast RPB6 and TFIIIA coding sequences are divergently transcribed and are separated by only 233 base pairs, providing the potential for coregulated expression of components of RNA polymerases and the 5S rRNA component of ribosomes [6].
  • The site of RNA polymerase III transcription factor A (TFIIIA) binding, mapped by determination of the G residues that are protected from methylation on exposure of the TFIIIA.5 S DNA complex to dimethyl sulfate, is coincident with the ICR [7].

Anatomical context of PZF1


Associations of PZF1 with chemical compounds

  • TFIIIA with internal deletions that removed residues 282 to 315, 316 to 334, 328 to 341, or 342 to 351 of the 81-amino-acid domain retained activity, whereas TFIIIA with a deletion of the short leucine-rich segment 352NGLNLLLN359 at the carboxyl-terminal end of this domain was devoid of activity [10].
  • It was sensitive to phenol extraction and resistant to RNase, and its target did not appear to be transcription factor IIIA [11].
  • In the fourth case, however, a substitution of phenylalanine for the wild-type leucine at position 148 in TFIIIA results in much larger compensating changes in the kinetics of complex assembly and dissociation [12].
  • Transcription factors TFIIIA and TFIIIC are able to be stably sequestered onto 5SrDNA-cellulose, but factor TFIIIB is not able to associate with the 5SrDNA-TFIIIA-TFIIIC complex in the presence of novobiocin [13].

Other interactions of PZF1

  • Instead, a complete transcription factor complex consisting of TFIIIA, TFIIIB, and TFIIIC needed to be formed before the addition of histones in order for the 5S chromatin to subsequently be transcribed by RNA polymerase III [14].
  • We propose a model for the regulation of yeast 5 S rRNA synthesis in which YL3 competes with the presumptive transcription factor IIIA analog for binding of 5 S rRNA [8].
  • This leads us to postulate that GAL4 does not possess a TFIIIA-like "Zn-finger" but forms a binuclear metal cluster involving all six cysteines in a "cloverleaf"-like array [15].

Analytical, diagnostic and therapeutic context of PZF1

  • We have combined this genetic assay with a simple and efficient method of mutagenesis that makes use of error-prone PCR and homologous recombination to generate and screen large numbers of TFIIIA mutants for those with altered 5 S rRNA gene-binding affinity [16].


  1. High level expression in E. coli and purification of yeast transcription factor IIIA. Ottonello, S., Ballabeni, A., Soncini, C., Dieci, G. Biochem. Biophys. Res. Commun. (1994) [Pubmed]
  2. S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors. Kassavetis, G.A., Braun, B.R., Nguyen, L.H., Geiduschek, E.P. Cell (1990) [Pubmed]
  3. Altering DNA-binding specificity of GAL4 requires sequences adjacent to the zinc finger. Corton, J.C., Johnston, S.A. Nature (1989) [Pubmed]
  4. Transcription factor IIIA induced bending of the Xenopus somatic 5S gene promoter. Schroth, G.P., Cook, G.R., Bradbury, E.M., Gottesfeld, J.M. Nature (1989) [Pubmed]
  5. The only essential function of TFIIIA in yeast is the transcription of 5S rRNA genes. Camier, S., Dechampesme, A.M., Sentenac, A. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  6. Genes encoding transcription factor IIIA and the RNA polymerase common subunit RPB6 are divergently transcribed in Saccharomyces cerevisiae. Woychik, N.A., Young, R.A. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  7. Transcription of the 5 S rRNA gene of Saccharomyces cerevisiae requires a promoter element at +1 and a 14-base pair internal control region. Challice, J.M., Segall, J. J. Biol. Chem. (1989) [Pubmed]
  8. Modulation of yeast 5 S rRNA synthesis in vitro by ribosomal protein YL3. A possible regulatory loop. Brow, D.A., Geiduschek, E.P. J. Biol. Chem. (1987) [Pubmed]
  9. Characterization of the RNA binding properties of transcription factor IIIA of Xenopus laevis oocytes. Romaniuk, P.J. Nucleic Acids Res. (1985) [Pubmed]
  10. A hydrophobic segment within the 81-amino-acid domain of TFIIIA from Saccharomyces cerevisiae is essential for its transcription factor activity. Rowland, O., Segall, J. Mol. Cell. Biol. (1998) [Pubmed]
  11. Regulation of the RNA polymerase I and III transcription systems in response to growth conditions. Clarke, E.M., Peterson, C.L., Brainard, A.V., Riggs, D.L. J. Biol. Chem. (1996) [Pubmed]
  12. Mutations in TFIIIA that increase stability of the TFIIIA-5 S rRNA gene complex: unusual effects on the kinetics of complex assembly and dissociation. Brady, K.L., Ponnampalam, S.N., Bumbulis, M.J., Setzer, D.R. J. Biol. Chem. (2005) [Pubmed]
  13. Novobiocin inhibits interactions required for yeast TFIIIB sequestration during stable transcription complex formation in vitro. Felts, S.J., Weil, P.A., Chalkley, R. Nucleic Acids Res. (1987) [Pubmed]
  14. Transcription factor requirements for in vitro formation of transcriptionally competent 5S rRNA gene chromatin. Felts, S.J., Weil, P.A., Chalkley, R. Mol. Cell. Biol. (1990) [Pubmed]
  15. The DNA binding domain of GAL4 forms a binuclear metal ion complex. Pan, T., Coleman, J.E. Biochemistry (1990) [Pubmed]
  16. Genetic analysis of Xenopus transcription factor IIIA. Bumbulis, M.J., Wroblewski, G., McKean, D., Setzer, D.R. J. Mol. Biol. (1998) [Pubmed]
WikiGenes - Universities