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Gene Review

oprM  -  multidrug ABC transporter

Pseudomonas aeruginosa PAO1

 
 
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Disease relevance of oprM

 

High impact information on oprM

  • Introduction of the oprM gene, but not the mexAB genes, into a P. aeruginosa multidrug-susceptible delta mexAB-oprM mutant increased resistance to quinolones, cephalosporins, erythromycin, and tetracycline [2].
  • Strains overexpressing OprM, but not those overexpressing OprJ, became hypersusceptible to TMP and SMX when oprM was inactivated [3].
  • To envisage the role of these proteins in antibiotic extrusion and resistance, we employed the gene replacement technique to construct mutants deficient in mexA, mexB, or oprM, and all possible combinations of these genes [4].
  • Introduction of the mexMN into the hypersensitive cells possessing oprM, but not into cells not possessing oprM, resulted in elevated MICs of chloramphenicol and thiamphenicol [5].
  • The results showed that oprD was relatively stable against carbapenem antibiotics. oprM was induced significantly by imipenem in only one strain and oprN was induced by imipenem in most of the strains [6].
 

Chemical compound and disease context of oprM

 

Biological context of oprM

  • Complementation of an oprM deletion mutant with the plasmid encoded histidine-tagged OprM protein restored antibiotic susceptibilities to wild-type levels, demonstrating functionality of recombinant OprM [7].
 

Associations of oprM with chemical compounds

  • Next, in four ciprofloxacin-selected nfxC-like mutants, levels of oprD, oprM and oprN mRNA were compared with those of their wild-type counterparts [8].

References

  1. MexAB-OprM hyperexpression in NalC-type multidrug-resistant Pseudomonas aeruginosa: identification and characterization of the nalC gene encoding a repressor of PA3720-PA3719. Cao, L., Srikumar, R., Poole, K. Mol. Microbiol. (2004) [Pubmed]
  2. Contribution of outer membrane efflux protein OprM to antibiotic resistance in Pseudomonas aeruginosa independent of MexAB. Zhao, Q., Li, X.Z., Srikumar, R., Poole, K. Antimicrob. Agents Chemother. (1998) [Pubmed]
  3. Multidrug efflux in intrinsic resistance to trimethoprim and sulfamethoxazole in Pseudomonas aeruginosa. Köhler, T., Kok, M., Michea-Hamzehpour, M., Plesiat, P., Gotoh, N., Nishino, T., Curty, L.K., Pechere, J.C. Antimicrob. Agents Chemother. (1996) [Pubmed]
  4. The role of mex-gene products in antibiotic extrusion in Pseudomonas aeruginosa. Yoneyama, H., Ocaktan, A., Tsuda, M., Nakae, T. Biochem. Biophys. Res. Commun. (1997) [Pubmed]
  5. Gene cloning and properties of the RND-type multidrug efflux pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa. Mima, T., Sekiya, H., Mizushima, T., Kuroda, T., Tsuchiya, T. Microbiol. Immunol. (2005) [Pubmed]
  6. Effect of carbapenems on the transcriptional expression of the oprD, oprM and oprN genes in Pseudomonas aeruginosa. Kolayli, F., Karadenizli, A., Savli, H., Ergen, K., Hatirnaz, O., Balikci, E., Budak, F., Vahaboglu, H. J. Med. Microbiol. (2004) [Pubmed]
  7. Overexpression, refolding, and purification of the histidine-tagged outer membrane efflux protein OprM of Pseudomonas aeruginosa. Charbonnier, F., Köhler, T., Pechère, J.C., Ducruix, A. Protein Expr. Purif. (2001) [Pubmed]
  8. Expression stability of six housekeeping genes: A proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR. Savli, H., Karadenizli, A., Kolayli, F., Gundes, S., Ozbek, U., Vahaboglu, H. J. Med. Microbiol. (2003) [Pubmed]
 
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