The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

ECs0819  -  endolysin

Escherichia coli O157:H7 str. Sakai

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of ECs0819

 

High impact information on ECs0819

  • The unusual N-terminal domain, rich in residues that are weakly hydrophobic, thus functions as a signal-arrest-release sequence, which first acts as a normal signal-arrest domain to direct the endolysin to the periplasm in membrane-tethered form and then allows it to be released as a soluble active enzyme in the periplasm [1].
  • Hydrolysis of cell walls of L. helveticus CNRZ 303 by the endolysin and biochemical analysis of the residues produced demonstrated that Mur-LH has N-acetylmuramidase activity [4].
  • The N-terminal region (about 80 amino acids) of Lys consists of two discrete regions (the signal-peptide-like domain and the DE domain containing putative active sites of endolysin) [6].
  • Most bacteriophages terminate infection by creating lesions in the cytoplasmic membrane, which not only cause immediate cell death but also allow escape of a phage-encoded endolysin [7].
  • Four genes identified within the late operon of PBSX show characteristics expected of a host cell lysis system; they are xepA, encoding an exported protein; xhlA, encoding a putative membrane-associated protein; xhlB, encoding a putative holin; and xlyA, encoding a putative endolysin [8].
 

Biological context of ECs0819

 

Anatomical context of ECs0819

  • Thus, the N-terminal domain of Lyz is both necessary and sufficient not only for export of this endolysin to the membrane but also for its release into the periplasm [1].
 

Associations of ECs0819 with chemical compounds

  • The antibacterial activity of the endolysin towards these three bacterial strains was marked when EDTA was added to the endolysin solution [12].
 

Analytical, diagnostic and therapeutic context of ECs0819

References

  1. A signal-arrest-release sequence mediates export and control of the phage P1 endolysin. Xu, M., Struck, D.K., Deaton, J., Wang, I.N., Young, R. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  2. The murein hydrolase of the bacteriophage phi3626 dual lysis system is active against all tested Clostridium perfringens strains. Zimmer, M., Vukov, N., Scherer, S., Loessner, M.J. Appl. Environ. Microbiol. (2002) [Pubmed]
  3. The missing link in phage lysis of gram-positive bacteria: gene 14 of Bacillus subtilis phage phi 29 encodes the functional homolog of lambda S protein. Steiner, M., Lubitz, W., Bläsi, U. J. Bacteriol. (1993) [Pubmed]
  4. Mur-LH, the broad-spectrum endolysin of Lactobacillus helveticus temperate bacteriophage phi-0303. Deutsch, S.M., Guezenec, S., Piot, M., Foster, S., Lortal, S. Appl. Environ. Microbiol. (2004) [Pubmed]
  5. Evidence for a holin-like protein gene fully embedded out of frame in the endolysin gene of Staphylococcus aureus bacteriophage 187. Loessner, M.J., Gaeng, S., Scherer, S. J. Bacteriol. (1999) [Pubmed]
  6. Molecular analysis of the lysis protein Lys encoded by Lactobacillus plantarum phage phig1e. Kakikawa, M., Yokoi, K.J., Kimoto, H., Nakano, M., Kawasaki, K., Taketo, A., Kodaira, K. Gene (2002) [Pubmed]
  7. The lambda holin accumulates beyond the lethal triggering concentration under hyperexpression conditions. Smith, D.L., Chang, C.Y., Young, R. Gene Expr. (1998) [Pubmed]
  8. Lysis genes of the Bacillus subtilis defective prophage PBSX. Krogh, S., Jørgensen, S.T., Devine, K.M. J. Bacteriol. (1998) [Pubmed]
  9. Functions involved in bacteriophage P2-induced host cell lysis and identification of a new tail gene. Ziermann, R., Bartlett, B., Calendar, R., Christie, G.E. J. Bacteriol. (1994) [Pubmed]
  10. The N-acetylmuramoyl-L-alanine amidase encoded by the Bacillus subtilis 168 prophage SP beta. Regamey, A., Karamata, D. Microbiology (Reading, Engl.) (1998) [Pubmed]
  11. The two-component lysis system of Staphylococcus aureus bacteriophage Twort: a large TTG-start holin and an associated amidase endolysin. Loessner, M.J., Gaeng, S., Wendlinger, G., Maier, S.K., Scherer, S. FEMS Microbiol. Lett. (1998) [Pubmed]
  12. Antibacterial activity of Bacillus amyloliquefaciens phage endolysin without holin conjugation. Morita, M., Tanji, Y., Mizoguchi, K., Soejima, A., Orito, Y., Unno, H. J. Biosci. Bioeng. (2001) [Pubmed]
  13. Cloning, expression and sequence analysis of an endolysin-encoding gene of Lactobacillus bulgaricus bacteriophage mv1. Boizet, B., Lahbib-Mansais, Y., Dupont, L., Ritzenthaler, P., Mata, M. Gene (1990) [Pubmed]
  14. Characterization of cryptic prophages (monocins) in Listeria and sequence analysis of a holin/endolysin gene. Zink, R., Loessner, M.J., Scherer, S. Microbiology (Reading, Engl.) (1995) [Pubmed]
  15. Identification and characterization of an endolysin encoded by the Streptomyces aureofaciens phage mu 1/6. Farkasovská, J., Godány, A., Vlcek, C. Folia Microbiol. (Praha) (2003) [Pubmed]
 
WikiGenes - Universities