Disease relevance of Hepatitis E

• Enhanced alpha1 microglobulin secretion from Hepatitis E virus ORF3-expressing human hepatoma cells is mediated by the tumor susceptibility gene 101 [1].
• The phosphorylated form of the ORF3 protein of hepatitis E virus interacts with its non-glycosylated form of the major capsid protein, ORF2 [2].
• Severe hemolysis and renal failure in glucose-6-phosphate dehydrogenase deficient patients with hepatitis E [3].
• The full-length putative structural proteins encoded by open reading frame 2 (ORF2) and ORF3 of hepatitis E virus have been cloned and expressed in recombinant baculovirus [4].
• Viral hepatitis in the 1990s, Part III: Hepatitis C, hepatitis E, and other viruses [5].

High impact information on Hepatitis E

• Computer-assisted assignment of functional domains in the nonstructural polyprotein of hepatitis E virus: delineation of an additional group of positive-strand RNA plant and animal viruses [6].
• Both inocula contained 27- ato 34-nm-diameter viruslike particles (VLPs) that were specifically aggregated by acute-phase ET-NANBH sera [7].
• WHsAg and DNA polymerase appeared to be more reliable indicators of infectivity than anti-WHc, woodchuck hepatitis e antigen (WHeAg) or anti-WHe [8].
• An earlier study in our laboratory has demonstrated that the ORF3 protein of the hepatitis E virus expedites the secretion of alpha1 microglobulin, an immunosuppressant molecule [1].
• The ORF3 protein of hepatitis E virus interacts with liver-specific alpha1-microglobulin and its precursor alpha1-microglobulin/bikunin precursor (AMBP) and expedites their export from the hepatocyte [9].

Chemical compound and disease context of Hepatitis E

• Hepatitis E virus: no evidence of parenteral transmission in Finland [10].
• METHODS: Serial serum samples of 198 patients with acute non-A, non-B hepatitis and a single serum specimen from 316 healthy subjects were tested for IgG and IgM antibodies to hepatitis E virus (anti-HEV) [11].
• Persistent and transient antibody responses to hepatitis E virus detected by western immunoblot using open reading frame 2 and 3 and glutathione S-transferase fusion proteins [12].
• Epidemic and sporadic hepatitis E virus transmission in West Kalimantan (Borneo), Indonesia [13].
• Swine hepatitis E virus (HEV) antigen was detected immunohistochemically in formalin-fixed, paraffin-embedded hepatic tissue from 30 naturally infected pigs [14].

Biological context of Hepatitis E

• The purpose of this study was to analyze partial nucleotide sequences and derived peptide sequences of hepatitis E virus (HEV) from two outbreaks of hepatitis E in Africa (Chad 1983-1984; Algeria 1978-1980) [15].
• Genetic heterogeneity of hepatitis E virus in Darfur, Sudan, and neighboring Chad [16].
• Recombinant chimeric protein C2 containing the N-terminal region of trpE (37 kilodaltons [kDa]) and the C-terminal half (46.8 kDa) of the polypeptide encoded by ORF2 of the hepatitis E virus (HEV) genome was used for the construction of a Western blot diagnostic test for IgG and IgM antibodies to the virus (anti-HEV) [17].
• BACKGROUND AND OBJECTIVES: Of 200 volunteer blood donors we had screened earlier for hepatitis E virus (HEV) RNA, using reverse-transcription polymerase chain reaction, 3 were positive, raising the possibility of transfusion-associated hepatitis E in areas endemic for this virus [18].
• METHODS: The plasmid containing the gene encoding cytotoxic T-lymphocyte antigen 4 (CTLA4), a surface molecule on T cells, was directly fused to the gene fragment HEVE2 coding for hepatitis E virus antigen by molecular engineering technology [19].

Gene context of Hepatitis E

• Cloning, sequencing, and expression of the hepatitis E virus (HEV) nonstructural open reading frame 1 (ORF1) [20].
• Promoter and intron-1 region polymorphisms in the IFNG gene in patients with hepatitis E [21].
• The ORF3 protein of hepatitis E virus binds to Src homology 3 domains and activates MAPK [22].
• The ORF2 protein of hepatitis E virus binds the 5' region of viral RNA [23].
• The 41-amino-acid C-terminal region of the hepatitis E virus ORF3 protein interacts with bikunin, a kunitz-type serine protease inhibitor [24].

Analytical, diagnostic and therapeutic context of Hepatitis E

• We demonstrated that the partially purified ORF-2 protein could be used successfully in a sensitive and specific enzyme-linked immunosorbent assay for the detection of antibodies to hepatitis E virus [25].
• Swine hepatitis E virus (HEV) was detected in formalin-fixed, paraffin-embedded tissues from naturally infected pigs by nested reverse transcription-polymerase chain reaction (RT-PCR) [26].

References