Disease relevance of fliE

• We have now demonstrated that the C. crescentus flbG and flaN promoters, which contain the Ntr/Nif type of consensus sequence, are utilized by purified Escherichia coli sigma 54 RNA polymerase (E sigma 54) in the presence of NRI and NRII but not by the purified sigma 70 RNA polymerase (E sigma 70) of E. coli [1].
• Escherichia coli sigma 54 RNA polymerase recognizes Caulobacter crescentus flbG and flaN flagellar gene promoters in vitro [1].
• Genetic analysis of three additional fla genes in Salmonella typhimurium [2].
• The strains with deletions starting within the prophage and ending in these fla genes were selected from among the heat-resistant survivors of the lysogen [3].
• Isolation of specialized lambda transducing bacteriophages for flagellar genes (fla) of Escherichia coli K-12 [3].

High impact information on fliE

• The flbG (hook operon), flaN, and flagellin gene operons, which are at the lowest levels of the hierarchy and expressed late in the cell cycle, contain Ntr-like promoters [4].
• The deletion strain lacks motility and belongs to the fla(-) mutant class, indicating that it is deficient in flagellar biogenesis [5].
• The fla gene cluster is involved in the biogenesis of flagella in Halobacterium salinarum [5].
• In region III, a previously unidentified gene flbC was located between hag and flaN [6].
• We cloned the fliE gene, obtained its DNA sequence, and identified its product.fliE was found to be a monocistronic transcriptional unit, adjacent to and divergent from the large fliF operon [7].

Biological context of fliE

• We identified a protein within the isolated hook-basal body complex as the fliE gene product on the basis of its size and comparison of its N-terminal amino acid sequence with that deduced from the gene sequence [7].
• The sequential transcription of flagellar (fla) genes in the Caulobacter crescentus cell cycle is controlled by the organization of these genes in a regulatory hierarchy of four levels (I-IV) [8].
• It was found that transcription of genes responsible for the formation and/or function of flagella (hag, fla, mot, che) decreased significantly at 40 degrees C. However, in the ftsI730 mutant at the nonpermissive temperature, or in penicillin G treated wild-type cells, cell division was blocked but formation of flagella continued [9].
• Restriction fragment length polymorphism analysis (RFLP) of fla A amplified product by using Bgl II enzyme classified 15 strains into 5 types [10].
• No analogs to the bacterial chemotactic and motility (che, mot, fla) genes, genes for a two-component signal transduction system, genes associated with gliding, or genomic homologs for the eukaryotic cytoskeleton and motor proteins were found in the Mollicutes [11].

Analytical, diagnostic and therapeutic context of fliE

• The engineered apo AI polypeptides were isolated and purified by affinity chromatography and assayed for their activator activity [12].
• We therefore used the method of site-directed mutagenesis of apo AI cDNAs using the overlap extension approach by the polymerase chain reaction [12].

References