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Gene Review

mnmG  -  5-methylaminomethyl-2-thiouridine...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3734, JW3719, gidA, mnmB, trmF
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Disease relevance of mnmG


High impact information on mnmG

  • We compared phylogenies of host genes (the nuclear EF-1alphaF2 and mitochondrial COI/II) and Blochmannia genes (16S ribosomal DNA [rDNA], groEL, gidA, and rpsB), totaling more than 7 kilobases for each of 16 Camponotus species [4].
  • The gidA* mutant releases a heat-stable, protease-resistant, small molecular weight molecule that acts in trans to inhibit aggregation and gene expression of wild-type cells during development [5].
  • In both seqA- and dam- cells, gidA and dnaA continued to be transcribed after initiation, whereas the inhibition of mioC transcription before initiation was unaltered [6].
  • Conversely, the active transcription of gidA and dnaA prior to initiation is consistent with their positive effects on initiation, and their shutdown after initiation could serve to limit premature reinitiation [7].
  • Consistent with the finding that GidA is essential in Escherichia coli, we were unable to obtain a gidA mutant in H. pylori despite evidence that insertional mutagenesis had occurred [8].

Biological context of mnmG

  • Site-specific recombination of a disrupted gidA gene into the chromosome resulted in the same phenotypic pattern as transposon insertion [2].
  • Wild-type levels of reporter expression were restored by supplying an intact gidA gene on a plasmid [2].
  • The mutations in the six group II mutants were complemented by introduction of the gidA gene, which may be involved in cell division [9].
  • The salA gene, a copy number suppressor of the syringomycin-deficient phenotype in gacS and gacA mutants, was also found to suppress the antibiotic-negative phenotypes of gidA mutants, suggesting that gidA might play some role in salA regulation [2].
  • Analysis of two virulence mutants of Pseudomonas syringae B728a revealed that the Tn 5 sites of insertion were within the gidA open reading frame (ORF) [2].

Regulatory relationships of mnmG

  • However, the mechanisms involved are different: regulation of transcription in the case of asnA and asnC and post-transcriptional control of gidA [10].

Other interactions of mnmG

  • Interestingly, high-performance liquid chromatography (HPLC) analysis shows that identical levels of the same undermodified form of U34 are present in tRNA hydrolysates from loss-of-function gidA and mnmE mutants [11].


  1. Genes and their organization in the replication origin region of the bacterial chromosome. Ogasawara, N., Yoshikawa, H. Mol. Microbiol. (1992) [Pubmed]
  2. Global regulation by gidA in Pseudomonas syringae. Kinscherf, T.G., Willis, D.K. J. Bacteriol. (2002) [Pubmed]
  3. Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho. Clark, M.A., Baumann, L., Baumann, P. Curr. Microbiol. (1998) [Pubmed]
  4. Host-symbiont stability and fast evolutionary rates in an ant-bacterium association: cospeciation of camponotus species and their endosymbionts, candidatus blochmannia. Degnan, P.H., Lazarus, A.B., Brock, C.D., Wernegreen, J.J. Syst. Biol. (2004) [Pubmed]
  5. GidA is an FAD-binding protein involved in development of Myxococcus xanthus. White, D.J., Merod, R., Thomasson, B., Hartzell, P.L. Mol. Microbiol. (2001) [Pubmed]
  6. DNA sequestration and transcription in the oriC region of Escherichia coli. Bogan, J.A., Helmstetter, C.E. Mol. Microbiol. (1997) [Pubmed]
  7. Correlation of gene transcription with the time of initiation of chromosome replication in Escherichia coli. Theisen, P.W., Grimwade, J.E., Leonard, A.C., Bogan, J.A., Helmstetter, C.E. Mol. Microbiol. (1993) [Pubmed]
  8. Characterization of Helicobacter pylori dapE and construction of a conditionally lethal dapE mutant. Karita, M., Etterbeek, M.L., Forsyth, M.H., Tummuru, M.K., Blaser, M.J. Infect. Immun. (1997) [Pubmed]
  9. Novel temperature-sensitive mutants of Escherichia coli that are unable to grow in the absence of wild-type tRNA6Leu. Nakayashiki, T., Inokuchi, H. J. Bacteriol. (1998) [Pubmed]
  10. AsnC, a multifunctional regulator of genes located around the replication origin of Escherichia coli, oriC. Kölling, R., Gielow, A., Seufert, W., Kücherer, C., Messer, W. Mol. Gen. Genet. (1988) [Pubmed]
  11. Further insights into the tRNA modification process controlled by proteins MnmE and GidA of Escherichia coli. Yim, L., Moukadiri, I., Bj??rk, G.R., Armengod, M.E. Nucleic Acids Res. (2006) [Pubmed]
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