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Gene Review

rbsK  -  ribokinase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3746, JW3731
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Disease relevance of rbsK

  • Mutants defective in this transport system, but normal for ribokinase , are able to grow normally on high concentrations of the sugar, indicating that there is at least a second, low-affinity transport system for ribose in E. coli K-12 [1].
  • The regulatory significance of these sequence features is discussed with respect to the rbs operon. rbsK has been cloned downstream from the Serratia marcescens trp promoter on a multicopy plasmid [2].
  • A 7-kb DNA fragment of Lactobacillus sakei, containing the rbsD, rbsK and rbsR genes was sequenced [3].

High impact information on rbsK

  • The sites of tagging correspond to ribosome stalling at the termination codon and at rare AGG codons encoding Arg-307 and Arg-309, the antepenultimate and C-terminal residues of E. coli ribokinase [4].
  • It includes the 3' terminus of rbsB (the gene for ribose-binding protein) and the entire rbsK gene, encoding ribokinase [2].
  • Ribokinase has been purified to homogeneity (216 mumol/min/mg) from a strain harboring this plasmid [2].
  • Amino acid compositions of native ribokinase and the C-terminal dodecapeptide agree with the predicted amino acid compositions, confirming the accuracy of the DNA sequence and the translation termination site [2].
  • Each subunit is composed of two domains, a larger alpha/beta domain and a smaller beta-sheet domain, similar to that of ribokinase and adenosine kinase, members of the PfkB family of carbohydrate kinases [5].

Biological context of rbsK


Associations of rbsK with chemical compounds


Other interactions of rbsK

  • Potential consensus promoter sequences and a stable stem-loop structure are present in the rbsB-rbsK intercistronic region [2].

Analytical, diagnostic and therapeutic context of rbsK


  1. Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12. Iida, A., Harayama, S., Iino, T., Hazelbauer, G.L. J. Bacteriol. (1984) [Pubmed]
  2. Ribokinase from Escherichia coli K12. Nucleotide sequence and overexpression of the rbsK gene and purification of ribokinase. Hope, J.N., Bell, A.W., Hermodson, M.A., Groarke, J.M. J. Biol. Chem. (1986) [Pubmed]
  3. Ribose utilization in Lactobacillus sakei: analysis of the regulation of the rbs operon and putative involvement of a new transporter. Stentz, R., Zagorec, M. J. Mol. Microbiol. Biotechnol. (1999) [Pubmed]
  4. Stop codons preceded by rare arginine codons are efficient determinants of SsrA tagging in Escherichia coli. Hayes, C.S., Bose, B., Sauer, R.T. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  5. Structure of Thermus thermophilus 2-Keto-3-deoxygluconate kinase: evidence for recognition of an open chain substrate. Ohshima, N., Inagaki, E., Yasuike, K., Takio, K., Tahirov, T.H. J. Mol. Biol. (2004) [Pubmed]
  6. Evidence for a catalytic Mg2+ ion and effect of phosphate on the activity of Escherichia coli phosphofructokinase-2: regulatory properties of a ribokinase family member. Parducci, R.E., Cabrera, R., Baez, M., Guixé, V. Biochemistry (2006) [Pubmed]
  7. Ribokinase from E. coli: expression, purification, and substrate specificity. Chuvikovsky, D.V., Esipov, R.S., Skoblov, Y.S., Chupova, L.A., Muravyova, T.I., Miroshnikov, A.I., Lapinjoki, S., Mikhailopulo, I.A. Bioorg. Med. Chem. (2006) [Pubmed]
  8. Activation of ribokinase by monovalent cations. Andersson, C.E., Mowbray, S.L. J. Mol. Biol. (2002) [Pubmed]
  9. Purification, characterization, and crystallization of Escherichia coli ribokinase. Sigrell, J.A., Cameron, A.D., Jones, T.A., Mowbray, S.L. Protein Sci. (1997) [Pubmed]
  10. Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8 A resolution: insights into a new family of kinase structures. Sigrell, J.A., Cameron, A.D., Jones, T.A., Mowbray, S.L. Structure (1998) [Pubmed]
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