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Gene Review

recQ  -  ATP-dependent DNA helicase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3816, JW5855
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Disease relevance of recQ

  • The recQ gene of Escherichia coli K12: primary structure and evidence for SOS regulation [1].
  • Most lambda bio or lambda pro transducing phages are formed by the recombination events at several hot spots, which are enhanced by the recQ mutation [2].
  • We studied the effect of recQ mutations on illegitimate recombination, which is an aberrant biological event related to the chromosomal abnormality in humans, and found that a variety of recQ mutations increased spontaneous illegitimate recombination by 20- to 300-fold and increased UV light-induced illegitimate recombination by 10- to 100-fold [2].

High impact information on recQ

  • The products of the recJ and recQ genes process the blocked replication forks before the resumption of replication and may affect the fidelity of the recovery process [3].
  • We discuss how cooperation of the recQ gene product and the recJ gene product brings about double-strand break repair accompanied by flanking crossing-over [4].
  • The Escherichia coli recQ gene, a member of the RecF recombination gene family, was set in an overexpression plasmid, and its product was purified to near-homogeneity [5].
  • Mutation of recF, recO or recQ produced recombinants in which this exchange tended to be closer to the origin, though the effect observed was rather small [6].
  • (iii) Mutations in two more SOS-inducible genes, recN and recQ, do not decrease the repair [7].

Biological context of recQ

  • Analysis of the sequence revealed an open reading frame thought to represent recQ, with a clockwise direction of transcription relative to the standard genetic map of E. coli K12 and having a coding capacity for a protein of Mr 68,350 [1].
  • The -10 region of the presumptive recQ promoter overlapped the putative terminator for the upstream gene pldA, and was immediately followed by a 15 bp stretch of DNA bearing a strong resemblance to the reported sequences of LexA repressor binding sites [1].
  • This latter finding suggested the possibility of SOS regulation of recQ gene expression, which was substantiated by experiments with recQ-lacZ fusions [1].
  • Similar relationships of recJ and recQ mutations are observed in cell survival after ultraviolet light irradiation, gamma-ray irradiation, and H2O2 treatment [4].
  • We show that conjugational recombination and DNA repair after UV and gamma irradiation in this mutant are highly dependent on recJ, partially dependent on recFOR, and independent of recQ [8].

Other interactions of recQ

  • The increase in average relative intensity is dependent on recO and recQ [9].


  1. The recQ gene of Escherichia coli K12: primary structure and evidence for SOS regulation. Irino, N., Nakayama, K., Nakayama, H. Mol. Gen. Genet. (1986) [Pubmed]
  2. RecQ DNA helicase is a suppressor of illegitimate recombination in Escherichia coli. Hanada, K., Ukita, T., Kohno, Y., Saito, K., Kato, J., Ikeda, H. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  3. Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination. Courcelle, J., Hanawalt, P.C. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  4. DNA double-strand break repair: genetic determinants of flanking crossing-over. Kusano, K., Sunohara, Y., Takahashi, N., Yoshikura, H., Kobayashi, I. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  5. Escherichia coli RecQ protein is a DNA helicase. Umezu, K., Nakayama, K., Nakayama, H. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  6. Conjugational recombination in Escherichia coli: genetic analysis of recombinant formation in Hfr x F- crosses. Lloyd, R.G., Buckman, C. Genetics (1995) [Pubmed]
  7. Genetic analysis of double-strand break repair in Escherichia coli. Takahashi, N.K., Kusano, K., Yokochi, T., Kitamura, Y., Yoshikura, H., Kobayashi, I. J. Bacteriol. (1993) [Pubmed]
  8. Effects of recJ, recQ, and recFOR mutations on recombination in nuclease-deficient recB recD double mutants of Escherichia coli. Ivancic-Bace, I., Salaj-Smic, E., Brcic-Kostic, K. J. Bacteriol. (2005) [Pubmed]
  9. UvrD Limits the Number and Intensities of RecA-Green Fluorescent Protein Structures in Escherichia coli K-12. Centore, R.C., Sandler, S.J. J. Bacteriol. (2007) [Pubmed]
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