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Gene Review

recN  -  recombination and repair protein

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2612, JW5416, radB
 
 
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Disease relevance of recN

  • Different effects of recJ and recN mutations on the postreplication repair of UV-damaged DNA in Escherichia coli K-12 [1].
  • Recombinant lambda phages that complement the recombination and repair defects of recN recBC sbcB mutants have been identified, and the recN gene has been cloned from these phages into a low copy-number plasmid [2].
  • Characterization of the spoIVB and recN loci of Bacillus subtilis [3].
  • To investigate the roles of the gonococcal recN and recJ genes in the recombination-based processes of the gonococcus, these genes were inactivated in the N. gonorrhoeae strain FA1090 [4].
  • Identification and disruption analysis of the recN gene in the extremely radioresistant bacterium Deinococcus radiodurans [5].
 

High impact information on recN

 

Biological context of recN

  • Overlapping functions of recD, recJ and recN provide evidence of three epistatic groups of genes in Escherichia coli recombination and DNA repair [9].
  • The basis for this phenotype was investigated by monitoring the properties associated with recN mutations in otherwise wild-type strains [2].
  • The minimal inducing dose in uvrA and recN mutants was lower than in the wild-type, whereas their SOS response was higher at all doses [10].
  • These results imply that recN cells are constitutively active for SOS mutagenesis functions [6].
  • Two independent genes, recN and spoIVB, along with their respective promoter and termination regions, were discovered and sequenced in the 3.4-kilobase region between the ahrC and spoOA genes at map position 216 in the Bacillus subtilis chromosome map [3].
 

Other interactions of recN

  • However, no synergistic interactions were discovered between recG258 and mutations in recF, recN, or recQ [11].
  • In the recBCsbcBC strain and isogenic recL and recN derivatives basal recA expressions were 3- to 5-fold higher than that of wild-type cells and were inducible by mitomycin C [12].
  • (iii) Mutations in two more SOS-inducible genes, recN and recQ, do not decrease the repair [13].
  • Mutation of the recN gene of Escherichia coli in a recBC sbcB genetic background blocks conjugational recombination and confers increased sensitivity to UV light and mitomycin C [2].
  • In contrast, frequencies of RecET-mediated illegitimate recombination were not affected by ruvAB, ruvC, recG, and recN mutations [14].
 

Analytical, diagnostic and therapeutic context of recN

  • This mutant, as well as a previously characterized recN mutant (recN262), showed a markedly decreased frequency of excision of Tn10 after treatment with UV or mitomycin C [15].
  • Northern blot experiments confirmed radiation activation of the recA and recN genes at a dose of 2 Gy [16].

References

  1. Different effects of recJ and recN mutations on the postreplication repair of UV-damaged DNA in Escherichia coli K-12. Wang, T.C., Smith, K.C. J. Bacteriol. (1988) [Pubmed]
  2. Repair of DNA double-strand breaks in Escherichia coli K12 requires a functional recN product. Picksley, S.M., Attfield, P.V., Lloyd, R.G. Mol. Gen. Genet. (1984) [Pubmed]
  3. Characterization of the spoIVB and recN loci of Bacillus subtilis. Van Hoy, B.E., Hoch, J.A. J. Bacteriol. (1990) [Pubmed]
  4. Roles of the recJ and recN genes in homologous recombination and DNA repair pathways of Neisseria gonorrhoeae. Skaar, E.P., Lazio, M.P., Seifert, H.S. J. Bacteriol. (2002) [Pubmed]
  5. Identification and disruption analysis of the recN gene in the extremely radioresistant bacterium Deinococcus radiodurans. Funayama, T., Narumi, I., Kikuchi, M., Kitayama, S., Watanabe, H., Yamamoto, K. Mutat. Res. (1999) [Pubmed]
  6. Escherichia coli cells defective for the recN gene display constitutive elevation of mutagenesis at 3,N(4)-ethenocytosine via an SOS-induced mechanism. Dunman, P.M., Ren, L., Rahman, M.S., Palejwala, V.A., Murphy, H.S., Volkert, M.R., Humayun, M.Z. Mol. Microbiol. (2000) [Pubmed]
  7. Nucleotide sequence and LexA regulation of the Escherichia coli recN gene. Rostas, K., Morton, S.J., Picksley, S.M., Lloyd, R.G. Nucleic Acids Res. (1987) [Pubmed]
  8. Conjugational recombination in Escherichia coli: genetic analysis of recombinant formation in Hfr x F- crosses. Lloyd, R.G., Buckman, C. Genetics (1995) [Pubmed]
  9. Overlapping functions of recD, recJ and recN provide evidence of three epistatic groups of genes in Escherichia coli recombination and DNA repair. Lloyd, R.G., Buckman, C. Biochimie (1991) [Pubmed]
  10. SOS induction by gamma-radiation in Escherichia coli strains defective in repair and/or recombination mechanisms. Breña-Valle, M., Serment-Guerrero, J. Mutagenesis (1998) [Pubmed]
  11. Genetic analysis of the recG locus of Escherichia coli K-12 and of its role in recombination and DNA repair. Lloyd, R.G., Buckman, C. J. Bacteriol. (1991) [Pubmed]
  12. Expression of the recA gene in recombination-deficient (rec-) strains of Escherichia coli. Chua, K.L., Mak, Y.K., Oliver, P. Biochimie (1993) [Pubmed]
  13. Genetic analysis of double-strand break repair in Escherichia coli. Takahashi, N.K., Kusano, K., Yokochi, T., Kitamura, Y., Yoshikura, H., Kobayashi, I. J. Bacteriol. (1993) [Pubmed]
  14. The role of UvrD in RecET-mediated illegitimate recombination in Escherichia coli. Shiraishi, K., Imai, Y., Yoshizaki, S., Tadaki, T., Ogata, Y., Ikeda, H. Genes Genet. Syst. (2006) [Pubmed]
  15. RecN SOS gene and induced precise excision of Tn10 in Escherichia coli. Chan, A., Levy, M.S., Nagel, R. Mutat. Res. (1994) [Pubmed]
  16. The use of radiation-induced bacterial promoters in anaerobic conditions: a means to control gene expression in clostridium-mediated therapy for cancer. Nuyts, S., Van Mellaert, L., Theys, J., Landuyt, W., Lambin, P., Anné, J. Radiat. Res. (2001) [Pubmed]
 
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