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Gene Review

recF  -  gap repair protein

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3692, JW3677, uvrF
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Disease relevance of recF


High impact information on recF


Chemical compound and disease context of recF


Biological context of recF


Associations of recF with chemical compounds

  • The results suggest that the streptomycin-resistant transformants arise exclusively via a recombinational pathway which is largely dependant on the recF gene product, and that this pathway is influenced by induction of the SOS response [11].
  • We have located the single nucleotide changes suffered in recA sequence of 2 recF partial suppressor mutations: srf-2020 at codon 121 and srf-801 at codon 257. srf-2020 changes codon 121 from threonine (ACC) to isoleucine (ATC). srf-801 changes codon 257 from glutamine (CAG) to proline (CCG) [12].
  • This uninduced mutagenesis is independent of the umuC+, recF+ and recA+ genes and we hypothesize that it is regionally targeted on pyrimidine cyclobutane or 6-4 dimers in the vicinity of the target CTC and GAG nucleotides [13].
  • Thus, we conclude that the induced (umuC and recF dependent) mutagenesis is locally targeted on pyrimidine cyclobutane or 6-4 dimers. glyU carried by M13 is equally subject to uninduced UV mutagenesis whether carried by mp8 or mp9 [13].

Regulatory relationships of recF


Other interactions of recF

  • The low level of transcription coming to the end of recF, and the highest activity of the gyrB promoter, as well as results obtained with several gyrB::lacZ translational fusions, support the conclusion that gyrB is predominantly expressed from its own promoter under standard growth conditions [14].
  • recF-dependent and recF recB-independent DNA gap-filling repair processes transfer dimer-containing parental strands to daughter strands in Escherichia coli K-12 uvrB [15].
  • The level of translation of recF-lacZ fusions is reduced 20-fold by nucleotides 49 to 146 of recF [16].
  • However, no synergistic interactions were discovered between recG258 and mutations in recF, recN, or recQ [17].
  • This in turn led to the discovery of several genes, including recF, recO, and recR, whose role in recombination would not otherwise have been detected [18].


  1. Transcriptional organization of the dnaN and recF genes of Escherichia coli K-12. Armengod, M.E., García-Sogo, M., Lambíes, E. J. Biol. Chem. (1988) [Pubmed]
  2. Sequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence for an essential phosphate binding loop. Sandler, S.J., Chackerian, B., Li, J.T., Clark, A.J. Nucleic Acids Res. (1992) [Pubmed]
  3. recF in Actinobacillus pleuropneumoniae. Loynds, B.M., Langford, P.R., Kroll, J.S. Nucleic Acids Res. (1992) [Pubmed]
  4. RecFOR proteins are essential for Pol V-mediated translesion synthesis and mutagenesis. Fujii, S., Isogawa, A., Fuchs, R.P. EMBO J. (2006) [Pubmed]
  5. Stationary phase induction of dnaN and recF, two genes of Escherichia coli involved in DNA replication and repair. Villarroya, M., Pérez-Roger, I., Macián, F., Armengod, M.E. EMBO J. (1998) [Pubmed]
  6. Nascent DNA processing by RecJ favors lesion repair over translesion synthesis at arrested replication forks in Escherichia coli. Courcelle, C.T., Chow, K.H., Casey, A., Courcelle, J. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  7. Properties of a mutant recA-encoded protein reveal a possible role for Escherichia coli recF-encoded protein in genetic recombination. Madiraju, M.V., Templin, A., Clark, A.J. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  8. Regulatory role of recF in the SOS response of Escherichia coli: impaired induction of SOS genes by UV irradiation and nalidixic acid in a recF mutant. Thoms, B., Wackernagel, W. J. Bacteriol. (1987) [Pubmed]
  9. DNA sequence and transcription of the region upstream of the E. coli gyrB gene. Adachi, T., Mizuuchi, K., Menzel, R., Gellert, M. Nucleic Acids Res. (1984) [Pubmed]
  10. Purification and preliminary characterization of the Escherichia coli K-12 recF protein. Griffin, T.J., Kolodner, R.D. J. Bacteriol. (1990) [Pubmed]
  11. Stimulation of recombination between homologous sequences on carcinogen-treated plasmid DNA and chromosomal DNA by induction of the SOS response in Escherichia coli K12. Abbott, P.J. Mol. Gen. Genet. (1985) [Pubmed]
  12. Cloning and preliminary characterization of srf-2020 and srf-801, the recF partial suppressor mutations which map in recA of Escherichia coli K-12. Wang, T.C., Madiraju, M.V., Templin, A., Clark, A.J. Biochimie (1991) [Pubmed]
  13. Genetic analysis of UV mutagenesis of the Escherichia coli glyU gene. Ciesla, Z., O'Brien, P., Clark, A.J. Mol. Gen. Genet. (1987) [Pubmed]
  14. An improved vector system for constructing transcriptional lacZ fusions: analysis of regulation of the dnaA, dnaN, recF and gyrB genes of Escherichia coli. Macián, F., Pérez-Roger, I., Armengod, M.E. Gene (1994) [Pubmed]
  15. recF-dependent and recF recB-independent DNA gap-filling repair processes transfer dimer-containing parental strands to daughter strands in Escherichia coli K-12 uvrB. Wang, T.V., Smith, K.C. J. Bacteriol. (1984) [Pubmed]
  16. Mutational analysis of sequences in the recF gene of Escherichia coli K-12 that affect expression. Sandler, S.J., Clark, A.J. J. Bacteriol. (1994) [Pubmed]
  17. Genetic analysis of the recG locus of Escherichia coli K-12 and of its role in recombination and DNA repair. Lloyd, R.G., Buckman, C. J. Bacteriol. (1991) [Pubmed]
  18. Homologous genetic recombination: the pieces begin to fall into place. Clark, A.J., Sandler, S.J. Crit. Rev. Microbiol. (1994) [Pubmed]
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