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Gene Review

glpF  -  glycerol facilitator

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3919, JW3898
 
 
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Disease relevance of glpF

 

High impact information on glpF

 

Chemical compound and disease context of glpF

  • In addition to determining the E. coli glpX sequence, we also sequenced the corresponding glpFKX region originating from Shigella flexneri, which after transfer into E. coli was instrumental in elucidating the function of glpF in glycerol transport (D. P. Richey and E. C. C. Lin, J. Bacteriol. 112:784-790, 1972) [7].
  • PsNIP-1, like several other NIPs, contains a tryptophan residue corresponding with Trp-48 in GlpF (the glycerol facilitator of Escherichia coli) that borders the selectivity filter in the permeation channel [8].
  • Antimonite is accumulated by the glycerol facilitator GlpF in Escherichia coli [9].
 

Biological context of glpF

 

Anatomical context of glpF

  • The glpF gene encodes a cytoplasmic membrane protein which facilitates the diffusion of glycerol into the cell [1].
  • These include the Escherichia coli glycerol facilitator, the major intrinsic protein from bovine lens fibre junction membranes, a plant tonoplast membrane protein, a soybean protein from the peribacteroid membrane, and a Drosophila neurogenic protein [13].
 

Associations of glpF with chemical compounds

  • Sequencing of the glpFKX region of this hybrid strain revealed an amber mutation instead of the tryptophan 215 codon in glpF [7].
  • The glpF protein allowed the rapid efflux of preequilibrated xylitol [12].
 

Regulatory relationships of glpF

 

Other interactions of glpF

 

Analytical, diagnostic and therapeutic context of glpF

References

  1. Structure and regulation of the glpFK operon encoding glycerol diffusion facilitator and glycerol kinase of Escherichia coli K-12. Weissenborn, D.L., Wittekindt, N., Larson, T.J. J. Biol. Chem. (1992) [Pubmed]
  2. Structure and gene-polypeptide relationships of the region encoding glycerol diffusion facilitator (glpF) and glycerol kinase (glpK) of Pseudomonas aeruginosa. Schweizer, H.P., Jump, R., Po, C. Microbiology (Reading, Engl.) (1997) [Pubmed]
  3. Cloning and sequencing of the Thermus aquaticus glycerol facilitator gene. Huang, H.S., Ito, K., Yoshimoto, T. DNA Seq. (2002) [Pubmed]
  4. Structure of a glycerol-conducting channel and the basis for its selectivity. Fu, D., Libson, A., Miercke, L.J., Weitzman, C., Nollert, P., Krucinski, J., Stroud, R.M. Science (2000) [Pubmed]
  5. Structure of aquaporin-2 vasopressin water channel. Bai, L., Fushimi, K., Sasaki, S., Marumo, F. J. Biol. Chem. (1996) [Pubmed]
  6. Molecular cloning and characterization of AqpZ, a water channel from Escherichia coli. Calamita, G., Bishai, W.R., Preston, G.M., Guggino, W.B., Agre, P. J. Biol. Chem. (1995) [Pubmed]
  7. Molecular analysis of the glpFKX regions of Escherichia coli and Shigella flexneri. Truniger, V., Boos, W., Sweet, G. J. Bacteriol. (1992) [Pubmed]
  8. Members of the aquaporin family in the developing pea seed coat include representatives of the PIP, TIP, and NIP subfamilies. Schuurmans, J.A., van Dongen, J.T., Rutjens, B.P., Boonman, A., Pieterse, C.M., Borstlap, A.C. Plant Mol. Biol. (2003) [Pubmed]
  9. Antimonite is accumulated by the glycerol facilitator GlpF in Escherichia coli. Sanders, O.I., Rensing, C., Kuroda, M., Mitra, B., Rosen, B.P. J. Bacteriol. (1997) [Pubmed]
  10. Mutational analysis of the Escherichia coli glpFK region with Tn5 mutagenesis and the polymerase chain reaction. Lupski, J.R., Zhang, Y.H., Rieger, M., Minter, M., Hsu, B., Ooi, B.G., Koeuth, T., McCabe, E.R. J. Bacteriol. (1990) [Pubmed]
  11. Characteristics of Fps1-dependent and -independent glycerol transport in Saccharomyces cerevisiae. Sutherland, F.C., Lages, F., Lucas, C., Luyten, K., Albertyn, J., Hohmann, S., Prior, B.A., Kilian, S.G. J. Bacteriol. (1997) [Pubmed]
  12. Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli. Heller, K.B., Lin, E.C., Wilson, T.H. J. Bacteriol. (1980) [Pubmed]
  13. Evolution of the MIP family of integral membrane transport proteins. Pao, G.M., Wu, L.F., Johnson, K.D., Höfte, H., Chrispeels, M.J., Sweet, G., Sandal, N.N., Saier, M.H. Mol. Microbiol. (1991) [Pubmed]
  14. Glycerol kinase of Escherichia coli is activated by interaction with the glycerol facilitator. Voegele, R.T., Sweet, G.D., Boos, W. J. Bacteriol. (1993) [Pubmed]
  15. Glycerol facilitator of Escherichia coli: cloning of glpF and identification of the glpF product. Sweet, G., Gandor, C., Voegele, R., Wittekindt, N., Beuerle, J., Truniger, V., Lin, E.C., Boos, W. J. Bacteriol. (1990) [Pubmed]
  16. The 6.9-A structure of GlpF: a basis for homology modeling of the glycerol channel from Escherichia coli. Stahlberg, H., Braun, T., de Groot, B., Philippsen, A., Borgnia, M.J., Agre, P., Kühlbrandt, W., Engel, A. J. Struct. Biol. (2000) [Pubmed]
 
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