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Gene Review

CMVs2gp2  -  ORF overlaps the C-terminal portion of the...

Cucumber mosaic virus

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Disease relevance of CMVs2gp2


High impact information on CMVs2gp2

  • The mechanisms underlying 2b suppression of RNA silencing are unknown [1].
  • In addition, our data indicate that the PTGS suppressor activity is important, but not sufficient, for virulence determination by the 2b protein [5].
  • By fusion with the green fluorescent protein, we now show that the CMV 2b protein localizes to the nuclei of tobacco suspension cells and whole plants via an arginine-rich nuclear localization signal, (22)KRRRRR(27) [5].
  • We report here that the tomato aspermy cucumovirus 2b protein (Tav2b), when expressed from the unrelated tobacco mosaic tobamovirus (TMV) RNA genome, activates strong host resistance responses to TMV in tobacco which are typical of the gene-for-gene disease resistance mechanism [6].
  • The structure and genetic diversity of a California Cucumber mosaic virus (CMV) population was assessed by single-strand conformation polymorphism and nucleotide sequence analyses of genomic regions 2b, CP, MP, and the 3' nontranslated region of RNA3 [7].

Biological context of CMVs2gp2

  • Both P1/HC-Pro and 2b reversed PTGS of Nii genes in 271-containing tobacco plants, but failed to reverse TGS of 35S-GUS transgenes in the same plant [3].
  • Analysis of various population genetics parameters and distribution of synonymous and nonsynonymous mutations revealed that different coding regions and even different parts of coding regions were under different evolutionary constraints, including a short region of the 2b gene for which evidence suggests possible positive selection [7].
  • We found that RNA 2 of the four ilarviruses sequenced to date encodes an additional conserved open reading frame (ORF), 2b, that overlaps the 3' end of the previously known ORF, 2a [2].
  • The cucumoviral 2b protein is a viral counterdefence factor that interferes with the establishment of virus-induced gene silencing in plants [8].
  • We recently reported the molecular characterization and functional analysis of an overlapping gene 2b encoded by RNA 2 of the Q strain of cucumber mosaic cucumovirus (Q-CMV) [9].

Anatomical context of CMVs2gp2

  • This fraction was also enriched in histones, suggesting that the CMV 2b protein associates preferentially with the host cell nucleus [8].
  • These results suggested that the 2b protein associates either with the nucleus or cytoskeleton of the host cell [8].
  • Notably, the Cucumber mosaic virus 2b protein, shown previously to function predominantly by preventing the long-distance transmission of systemic silencing signals, was a very strong silencing suppressor in the protoplasts [10].

Associations of CMVs2gp2 with chemical compounds

  • Further analysis showed that the 2b protein was enriched in a fraction that sedimented through a 2.2 M sucrose cushion [8].
  • Subcellular fractionation experiments on CMV-infected tobacco leaf tissue showed that the Fny-CMV 2b protein accumulated within a fraction that sedimented at forces of less than 5000 g and that the 2b protein was solubilized only by treatment with urea or SDS [8].


  1. Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Zhang, X., Yuan, Y.R., Pei, Y., Lin, S.S., Tuschl, T., Patel, D.J., Chua, N.H. Genes Dev. (2006) [Pubmed]
  2. Ilarviruses encode a Cucumovirus-like 2b gene that is absent in other genera within the Bromoviridae. Xin, H.W., Ji, L.H., Scott, S.W., Symons, R.H., Ding, S.W. J. Virol. (1998) [Pubmed]
  3. Plant viral suppressors of post-transcriptional silencing do not suppress transcriptional silencing. Marathe, R., Smith, T.H., Anandalakshmi, R., Bowman, L.H., Fagard, M., Mourrain, P., Vaucheret, H., Vance, V.B. Plant J. (2000) [Pubmed]
  4. Functional replacement of the tobacco rattle virus cysteine-rich protein by pathogenicity proteins from unrelated plant viruses. Liu, H., Reavy, B., Swanson, M., MacFarlane, S.A. Virology (2002) [Pubmed]
  5. Suppression of post-transcriptional gene silencing by a plant viral protein localized in the nucleus. Lucy, A.P., Guo, H.S., Li, W.X., Ding, S.W. EMBO J. (2000) [Pubmed]
  6. Strong host resistance targeted against a viral suppressor of the plant gene silencing defence mechanism. Li, H.W., Lucy, A.P., Guo, H.S., Li, W.X., Ji, L.H., Wong, S.M., Ding, S.W. EMBO J. (1999) [Pubmed]
  7. Molecular population genetics of Cucumber mosaic virus in California: evidence for founder effects and reassortment. Lin, H.X., Rubio, L., Smythe, A.B., Falk, B.W. J. Virol. (2004) [Pubmed]
  8. Subcellular distribution analysis of the cucumber mosaic virus 2b protein. Mayers, C.N., Palukaitis, P., Carr, J.P. J. Gen. Virol. (2000) [Pubmed]
  9. In vivo expression of an overlapping gene encoded by the cucumoviruses. Shi, B.J., Ding, S.W., Symons, R.H. J. Gen. Virol. (1997) [Pubmed]
  10. Dissecting RNA silencing in protoplasts uncovers novel effects of viral suppressors on the silencing pathway at the cellular level. Qi, Y., Zhong, X., Itaya, A., Ding, B. Nucleic Acids Res. (2004) [Pubmed]
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