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MeSH Review

Serial Extraction

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High impact information on Serial Extraction

  • Results from serial extraction experiments have shown that this protein exists in two distinct pools: a Tris-soluble, and a Tris-insoluble but urea- or SDS-soluble one [1].
  • 0. Partial purification was achieved by serial extraction of parasites in Triton X-100 followed by Sephadex G-150 affinity chromatography [2].
  • SL was subjected to serial extraction with four organic solvents of increasing polarity (n-hexane, chloroform, ethyl acetate, n-butanol and water) [3].
  • The proteins were analyzed using a serial extraction method which liberates proteins from distinct pools; first, proteins were extracted in 4.0 M GuHCl (G1), followed by the extraction of mineral-bound protein with 0.5 M EDTA (E), and finally the protein previously masked by the mineral was released by a second 4.0 M GuHCl extraction (G2) [4].
  • Solid and liquid samples (0.2-1 g dry weight and 10 ml) were subjected to alkaline saponification followed by serial extraction using pentane (3 x 10 ml) [5].

Anatomical context of Serial Extraction

  • Prolactin (PRL) was purified from freshly frozen pituitary glands of water buffaloes (Bubalus bubalis) by a combination of existing procedures of Ellis and Jiang and Wilhelmi involving serial extraction of different pituitary proteins [6].
  • Mandibular dental casts and lateral cephalograms from 31 subjects who had undergone serial extraction without orthodontic treatment were analyzed at 3 stages: before extraction of the deciduous canines (T1), after extraction of first premolars (T2), and at the end of the observation period (T3) [7].
  • To determine whether membrane or cytoskeleton interactions are primarily responsible for anchoring annexin II2p11(2) in the cell cortex, we subjected Madin-Darby canine kidney (MDCK) cells to serial extractions using different detergents and identified annexin II and p11 in the different fractions employing specific antibodies [8].

Associations of Serial Extraction with chemical compounds


Gene context of Serial Extraction


  1. Differentiation-dependent changes in the solubility of a 195-kD protein in human epidermal keratinocytes. Ma, A.S., Sun, T.T. J. Cell Biol. (1986) [Pubmed]
  2. Description and characterization of a surface lectin from Giardia lamblia. Farthing, M.J., Pereira, M.E., Keusch, G.T. Infect. Immun. (1986) [Pubmed]
  3. Investigation on Semecarpus Lehyam--a Siddha medicine for breast cancer. Sowmyalakshmi, S., Nur-E-Alam, M., Akbarsha, M.A., Thirugnanam, S., Rohr, J., Chendil, D. Planta (2005) [Pubmed]
  4. The distribution of soluble, mineral-bound, and matrix-bound proteins in osteoporotic and normal bones. Grynpas, M.D., Tupy, J.H., Sodek, J. Bone (1994) [Pubmed]
  5. Mass spectrometric determination of ergosterol in a prairie natural wetland. Headley, J.V., Peru, K.M., Verma, B., Robarts, R.D. Journal of chromatography. A. (2002) [Pubmed]
  6. Physico-chemical and immunological characteristics of pituitary prolactin from water buffaloes (Bubalus bubalis). Chadha, N., Kohli, R., Kumari, G.L., Muralidhar, K. Mol. Cell. Biochem. (1991) [Pubmed]
  7. Effect of serial extraction alone on crowding: spontaneous changes in dentition after serial extraction. Yoshihara, T., Matsumoto, Y., Suzuki, J., Sato, N., Oguchi, H. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics. (2000) [Pubmed]
  8. The annexin II2p11(2) complex is the major protein component of the triton X-100-insoluble low-density fraction prepared from MDCK cells in the presence of Ca2+. Harder, T., Gerke, V. Biochim. Biophys. Acta (1994) [Pubmed]
  9. Quantitation and stability of fumonisins B1 and B2 in milk. Maragos, C.M., Richard, J.L. Journal of AOAC International. (1994) [Pubmed]
  10. Heterogeneity in buffalo pituitary prolactin. Khurana, S., Muralidhar, K. Mol. Cell. Biochem. (1997) [Pubmed]
  11. Mutagenicity of products from coal gasification and liquefaction in the Salmonella/microsome assay. Schoeny, R., Warshawsky, D., Hollingsworth, L., Hund, M., Moore, G. Environmental mutagenesis. (1981) [Pubmed]
  12. Extraction or non-extraction the active role of the general practitioner. Part 4: serial extraction. Alexander, C.M., Jacobs, J.D. Texas dental journal. (1977) [Pubmed]
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