High impact information on Serial Extraction

• Results from serial extraction experiments have shown that this protein exists in two distinct pools: a Tris-soluble, and a Tris-insoluble but urea- or SDS-soluble one [1].
• 0. Partial purification was achieved by serial extraction of parasites in Triton X-100 followed by Sephadex G-150 affinity chromatography [2].
• SL was subjected to serial extraction with four organic solvents of increasing polarity (n-hexane, chloroform, ethyl acetate, n-butanol and water) [3].
• The proteins were analyzed using a serial extraction method which liberates proteins from distinct pools; first, proteins were extracted in 4.0 M GuHCl (G1), followed by the extraction of mineral-bound protein with 0.5 M EDTA (E), and finally the protein previously masked by the mineral was released by a second 4.0 M GuHCl extraction (G2) [4].
• Solid and liquid samples (0.2-1 g dry weight and 10 ml) were subjected to alkaline saponification followed by serial extraction using pentane (3 x 10 ml) [5].

Anatomical context of Serial Extraction

• Prolactin (PRL) was purified from freshly frozen pituitary glands of water buffaloes (Bubalus bubalis) by a combination of existing procedures of Ellis and Jiang and Wilhelmi involving serial extraction of different pituitary proteins [6].
• Mandibular dental casts and lateral cephalograms from 31 subjects who had undergone serial extraction without orthodontic treatment were analyzed at 3 stages: before extraction of the deciduous canines (T1), after extraction of first premolars (T2), and at the end of the observation period (T3) [7].
• To determine whether membrane or cytoskeleton interactions are primarily responsible for anchoring annexin II2p11(2) in the cell cortex, we subjected Madin-Darby canine kidney (MDCK) cells to serial extractions using different detergents and identified annexin II and p11 in the different fractions employing specific antibodies [8].

Associations of Serial Extraction with chemical compounds

• The more sensitive LC method involves serial extraction of milk with methanol-acetone and strong anion exchange followed by derivatization with naphthalene-2,3-dicarboxaldehyde [9].
• The Ellis procedure of serial extraction of gonadotropins and growth hormone (GH) followed by alkaline ethanol extraction was adopted to process freshly frozen buffalo pituitaries [10].
• Fractionation of all samples was undertaken by serial extraction with organic solvents of increasing polarity (hexane, toluene, methylene chloride, acetonitrile) [11].

Gene context of Serial Extraction

• Extraction or non-extraction the active role of the general practitioner. Part 4: serial extraction [12].

References