Disease relevance of Herpesvirus 4, Equid

• Alignment of EHV-4 gB with HSV-1 (KOS) gB further revealed that four potential N-linked glycosylation sites and all 10 cysteine residues on the external surface of the molecules are perfectly conserved, suggesting that the proteins possess similar secondary and tertiary structures [1].
• Hamsters were immunized with either an affinity-purified preparation of equid herpesvirus 1 (EHV-1) glycoprotein 13 (gp13) or synthetic peptides representing three sequences within the homologous glycoprotein of EHV-4, resulting in the production of anti-peptide (in the case of peptide-immunized animals) or antivirus antibodies [2].
• The EHV-4 gH exhibits features previously reported to be conserved throughout the gH polypeptides of herpesviruses of all three subgroups [3].
• Tracheal clearance rates (TCR) were determined before, during illness and following recovery in horses exposed to equine rhinovirus (ERhV-2), equine influenza virus (EIV) and equine herpesvirus (EHV-4) by means of lateral scintigraphs made following an injection of technetium-99m sulphide colloid into the tracheal lumen [4].

High impact information on Herpesvirus 4, Equid

• A glycoprotein M-deleted equid herpesvirus 4 is severely impaired in virus egress and cell-to-cell spread [5].
• A 24-amino-acid repeat sequence expressed as a glutathione S-transferase fusion protein specifically reacted as well as the type-specific region with sera from foals infected with EHV-4 [6].
• We have determined the genomic location and nucleotide sequence of the equine herpesvirus 4 thymidine kinase (TK) gene [7].
• The equine herpesvirus 4 (EHV-4) gene glycoprotein H (gH) gene homologue was localized by virtue of the conserved genomic position of this gene throughout members of the herpesvirus family [3].
• Antibodies to these same three (EHV-4) or four (EHV-1) proteins, together with antibodies to the major capsid protein and proteins of 67K (EHV-4) and 87K (EHV-1) were detected in response to primary infection (control foal 2) and these sera had high neutralizating antibody titres [8].

Chemical compound and disease context of Herpesvirus 4, Equid

• Equine herpesvirus-4 and feline herpesvirus-1, both enveloped viruses, were not recovered at any time > or = 1 h post inoculation in fluorescein [9].

Biological context of Herpesvirus 4, Equid

• In order to identify the role of the equine herpesvirus type 4 (EHV-4) glycoprotein I (gI) and E (gE) genes in determining viral virulence and their affect on the infection cycle, we constructed an EHV-4 recombinant strain containing a deletion in both gI and gE genes and its revertant [10].

Gene context of Herpesvirus 4, Equid

• The EHV-4 gB homologue consists of a 112K glycoprotein which is cleaved to give glycoproteins of 74K and 61K which are also linked by disulphide bonds [11].
• Equine herpesviruses type 1 (EHV-1) and type 4 (EHV-4) induce a complement receptor protein on the surface of infected cells capable of binding to the third component of complement (C3) [12].
• Primers for PCR were designed from aligned nucleotide sequences of glycoprotein B genes of EHV-1 and EHV-4 to amplify specific regions for EHV-1 or EHV-4 or a common region of both viruses [13].

Analytical, diagnostic and therapeutic context of Herpesvirus 4, Equid

• Primers for PCR were designed from the sequences of the glycoprotein B genes of EHV-1 and EHV-4 [14].

References