Induction of surfactant protein a expression by cortisol facilitates prostaglandin synthesis in human chorionic trophoblasts.
Context: Surfactant protein A (SP-A) may be an important link between the maturation of fetal organs and the initiation of parturition. However, the local expression of SP-A and the effect of SP-A on prostaglandin synthesis in human fetal membranes have not been resolved. Objective: Our objective was to examine SP-A expression and the effect of SP-A on prostaglandin synthesis in human fetal membranes. Design: SP-A expression was examined with immunohistochemistry and PCR. The effect of SP-A on prostaglandin synthesis was investigated in cultured human chorionic trophoblasts. Patients: Patients were normal-term pregnant women undergoing elective cesarean sections. Results: Both SP-A protein and mRNA were present in amnion epithelial cells, fibroblasts, and chorionic trophoblasts. Cortisol (10(-7) and 10(-6) m, 24 h) induced SP-A expression in cultured chorionic trophoblasts, which could be blocked by the glucocorticoid receptor antagonist RU486. Treatment of chorionic trophoblasts with SP-A (10-100 mug/ml, 24 h) caused a dose-dependent increase of prostaglandin E(2) release and an induction of cyclooxygenase type 2 but not cytosolic phospholipase A(2) and microsomal prostaglandin E synthase expression. Conclusions: SP-A can be synthesized locally in human fetal membranes, which can be induced by glucocorticoids. SP-A appeared to induce prostaglandin E(2) synthesis in chorionic trophoblasts via induction of cyclooxygenase type 2 expression.[1]References
- Induction of surfactant protein a expression by cortisol facilitates prostaglandin synthesis in human chorionic trophoblasts. Sun, K., Brockman, D., Campos, B., Pitzer, B., Myatt, L. J. Clin. Endocrinol. Metab. (2006) [Pubmed]
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