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Chemical Compound Review:

Me2BAPTA 2-[[2-[2-[2- (bis(carboxymethyl)amino)- 5...

Synonyms: CHEBI:60889, AC1Q5WIB, CTK8D6121, AR-1D0257, LS-187539, ...

Carpenter-Deyo, L. et al., Rosales, C. et al., Wu-Wong, J.R. et al., Suchard, S.J. et al., Forehand, J.R. et al., et al.

Wu-Wong, Berg, Dayton,

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Disease relevance of maptam

To determine whether incubation for several hours with intracellular Ca++ indicators caused toxicity to freshly isolated hepatocytes from rats, cells were incubated under 95% O2-5% CO2 in medium containing 2 mM Ca++ and the acetoxymethyl (AM) esters of Quin 2, Indo 1, Fluo 3, 5,5'-Dimethyl BAPTA, 4,4'-Difluoro BAPTA or Fura 2 for up to 5 hr [1].

High impact information on maptam

Addition to cells of the intracellular Ca2+ chelator MAPTAM before preincubation with LPS blocked the changes in [Ca2+]i and the enhanced respiratory burst that characterize LPS priming [2].
Protein kinase C inhibitor, Ro 31-8220, or a calcium chelator 5,5'-dimethyl-BAPTA shifted the concentration-response curve of convulxin-induced platelet aggregation to the right [3].
Modulators of intracellular calcium homeostasis, MAPTAM, and TMB-8 blocked ookinete development as did the calmodulin (CaM) antagonists W-7 and calmidazolium [4].
In 5,5'-dimethyl-BAPTA-treated platelets, Y-27632 and HA 1077 completely abolished both ADP-induced platelet shape change and myosin light chain phosphorylation [5].
Exposing PMN to the intracellular Ca2+ chelators MAPTAM or BAPTA significantly reduced H2O2 generation in response to the receptor-mediated agonists, FMLP or GM-CSF, but did not affect PMA-stimulated H2O2 generation [6].

Biological context of maptam

When PMN were loaded with MAPTAM, a cell permeant analog of EGTA that prevented any rise in [Ca2+]i, they showed no FMLP-stimulated phagocytosis but had a normal phagocytic response to PAF and phorbol dibutyrate [7].

Anatomical context of maptam

In contrast to phospholipase inhibition, intracellular Ca2+ chelation with MAPTAM did not affect monocyte adherence but did inhibit monocyte spreading [8].
Depletion of intracellular Ca(2+) by treating 3T3-L1 adipocytes with EDTA and 1,2-bis(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetra-acetic acid tetra-acetoxymethyl ester (MAPTAM) did not have a significant effect on ET-1-induced glucose uptake [9].

Gene context of maptam

Addition of MAPTAM after FMLP priming abolished enhanced ingestion, even in the presence of optimal extracellular Ca2+ [7].

References

Toxicity to isolated hepatocytes caused by the intracellular calcium indicator, Quin 2. Carpenter-Deyo, L., Duimstra, J.R., Hedstrom, O., Reed, D.J. J. Pharmacol. Exp. Ther. (1991) [Pubmed]
Lipopolysaccharide priming of human neutrophils for an enhanced respiratory burst. Role of intracellular free calcium. Forehand, J.R., Pabst, M.J., Phillips, W.A., Johnston, R.B. J. Clin. Invest. (1989) [Pubmed]
Glycoprotein VI-mediated platelet fibrinogen receptor activation occurs through calcium-sensitive and PKC-sensitive pathways without a requirement for secreted ADP. Quinton, T.M., Ozdener, F., Dangelmaier, C., Daniel, J.L., Kunapuli, S.P. Blood (2002) [Pubmed]
Inhibition of Ca2+/calmodulin-dependent protein kinase blocks morphological differentiation of plasmodium gallinaceum zygotes to ookinetes. Silva-Neto, M.A., Atella, G.C., Shahabuddin, M. J. Biol. Chem. (2002) [Pubmed]
Platelet shape change is mediated by both calcium-dependent and -independent signaling pathways. Role of p160 Rho-associated coiled-coil-containing protein kinase in platelet shape change. Paul, B.Z., Daniel, J.L., Kunapuli, S.P. J. Biol. Chem. (1999) [Pubmed]
Exocytosis of a subpopulation of specific granules coincides with H2O2 production in adherent human neutrophils. Suchard, S.J., Boxer, L.A. J. Immunol. (1994) [Pubmed]
Two mechanisms for IgG Fc-receptor-mediated phagocytosis by human neutrophils. Rosales, C., Brown, E.J. J. Immunol. (1991) [Pubmed]
Phospholipase activation during monocyte adherence and spreading. Lefkowith, J.B., Lennartz, M.R., Rogers, M., Morrison, A.R., Brown, E.J. J. Immunol. (1992) [Pubmed]
Endothelin-stimulated glucose uptake: effects of intracellular Ca(2+), cAMP and glucosamine. Wu-Wong, J.R., Berg, C.E., Dayton, B.D. Clin. Sci. (2002) [Pubmed]

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