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Chemical Compound Review

Fluo-3     2-[[2-[2-[2- (bis(carboxymethyl)amino)-5- (2...

Synonyms: Fluo 3, Fluo-3-AM, CHEMBL509919, CHEBI:5107, AC1L2XTQ, ...
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Disease relevance of Fluo-3

  • Fluo-3 signals associated with potassium contractures in single amphibian muscle fibres [1].
  • The effect of endotoxemia on concanavalin A induced alterations in cytoplasmic free calcium in rat spleen cells as determined with Fluo-3 [2].
  • We examined the intracellular Ca(2+) response in primary cultured rat cortical neurons and human neuroblastoma SH-SY5Y cells by Fluo 3 fluorescence imaging analysis [3].
  • Pertussis toxin effects on chemoattractant-induced response heterogeneity in human PMNs utilizing Fluo-3 and flow cytometry [4].
  • In acutely dissociated mouse dorsal root ganglion neurones, SQA-neuropeptide FF reduced by 40% the depolarisation-induced rise in intracellular Ca2+ as measured with the Ca2+ indicator, Fluo-3 [5].

High impact information on Fluo-3


Biological context of Fluo-3

  • Fluo-3 also is introduced into individual submicrometer diameter processes of thapsigargin-treated progenitor cells, and a plasmid vector cDNA construct (pRAY 1), expressing the green fluorescent protein, is electroporated into cultured single COS 7 cells [11].
  • Furthermore, the release of intracellular Ca(2+), as assayed by the indicator dye, Fluo-3, had similar kinetics and voltage dependence for alpha(1S) and alpha(1S)-scr [12].
  • Because of cell dislocation during vigorous trichocyst exocytosis, 7S cells could be reasonably analysed only by CLSM after Fluo-3 injection [13].
  • Calcium signaling was measured in Fura-2-loaded cells on coverslips by dual-wavelength spectrofluorometry and in Fluo-3-loaded cells by confocal fluorescence laser microscopy [14].
  • S. ingentis oocytes were spawned into Mg(2+)-free seawater and microinjected with the fluorescent Ca2+ indicator Fluo-3 to study the effects of added Mg2+ on intracellular Ca2+ levels [15].

Anatomical context of Fluo-3

  • Fluo-3 was used as a Ca2+ indicator in either smooth muscle or endothelial cells of arterioles from the hamster cheek pouch [16].
  • The organ was loaded with the fluorescent Ca2+ indicator Fluo-3, and the cochlear electric responses to low-level tones were recorded through a microelectrode in the scala media [17].
  • Preloading of WIF-B cells with a membrane-permeable ester of the calcium-dependent fluorescent indicator, Fluo-3, was followed by Mrp2-mediated secretion of the amphiphilic anion, Fluo-3, into the apical vacuoles [18].
  • The Fluo-3 fluorescence in these calcium-containing organelles (CCOs) was transiently quenched by exposure to Mn2+, indicating that the dye is a genuine [Ca2+] reporter and is not just a site of accumulating Fluo-3 dye [19].
  • The measurement of cytosolic Ca2+ concentration by cytofluorimetry showed that Fluo-3 loaded macrophages from cirrhotic rats had a greater cytosolic CA2+ concentration than macrophages from control animals both in basal conditions and after A23187 stimulation [20].

Associations of Fluo-3 with other chemical compounds


Gene context of Fluo-3

  • Intracellular calcium, measured using Fluo-3 and Fura-2 fluorescent calcium indicator dyes, was somewhat lower, but not statistically different in 435/BRMS1 compared with parental cell [26].
  • Fluo 3 secretion into the apical vacuoles was inhibited by cyclosporin A but not by selective inhibitors of multidrug resistance 1 P-glycoprotein [27].
  • Examination of miniature EPSCs (mEPSCs) indicated that the decay kinetics of the NMDAR component was markedly slowed by the intracellular perfusion of exogenous calcium buffers (BAPTA or Fluo-3) [28].
  • Then the increase in intracellular Ca(2+) concentration after stimulation with SDF-1 was examined by the FLIPR and the flow cytometer, which monitored the change in green fluorescence intensity of Ca(2+)-bound Fluo-3 [29].
  • Like the Fluo-3 pump, mrp1 protein expression was enhanced by IL-2 [30].

Analytical, diagnostic and therapeutic context of Fluo-3


  1. Fluo-3 signals associated with potassium contractures in single amphibian muscle fibres. Caputo, C., Bolaños, P. J. Physiol. (Lond.) (1994) [Pubmed]
  2. The effect of endotoxemia on concanavalin A induced alterations in cytoplasmic free calcium in rat spleen cells as determined with Fluo-3. Hagar, A.F., Spitzer, J.A. Cell Calcium (1992) [Pubmed]
  3. Honokiol and magnolol induce Ca2+ mobilization in rat cortical neurons and human neuroblastoma SH-SY5Y cells. Zhai, H., Nakade, K., Mitsumoto, Y., Fukuyama, Y. Eur. J. Pharmacol. (2003) [Pubmed]
  4. Pertussis toxin effects on chemoattractant-induced response heterogeneity in human PMNs utilizing Fluo-3 and flow cytometry. Omann, G.M., Harter, J.M. Cytometry. (1991) [Pubmed]
  5. Biochemical, cellular and pharmacological activities of a human neuropeptide FF-related peptide. Gelot, A., Mazarguil, H., Dupuy, P., Francés, B., Gouardères, C., Roumy, M., Zajac, J.M. Eur. J. Pharmacol. (1998) [Pubmed]
  6. The light response of Drosophila photoreceptors is accompanied by an increase in cellular calcium: effects of specific mutations. Peretz, A., Suss-Toby, E., Rom-Glas, A., Arnon, A., Payne, R., Minke, B. Neuron (1994) [Pubmed]
  7. Extracellular polyamines regulate fluid secretion in rat colonic crypts via the extracellular calcium-sensing receptor. Cheng, S.X., Geibel, J.P., Hebert, S.C. Gastroenterology (2004) [Pubmed]
  8. Cyclic nucleotide-gated channels on the flagellum control Ca2+ entry into sperm. Wiesner, B., Weiner, J., Middendorff, R., Hagen, V., Kaupp, U.B., Weyand, I. J. Cell Biol. (1998) [Pubmed]
  9. Nuclear calcium signalling by individual cytoplasmic calcium puffs. Lipp, P., Thomas, D., Berridge, M.J., Bootman, M.D. EMBO J. (1997) [Pubmed]
  10. Lymphocyte adhesion-dependent calcium signaling in human endothelial cells. Pfau, S., Leitenberg, D., Rinder, H., Smith, B.R., Pardi, R., Bender, J.R. J. Cell Biol. (1995) [Pubmed]
  11. Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes. Lundqvist, J.A., Sahlin, F., Aberg, M.A., Strömberg, A., Eriksson, P.S., Orwar, O. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  12. Excitation-contraction coupling is not affected by scrambled sequence in residues 681-690 of the dihydropyridine receptor II-III loop. Proenza, C., Wilkens, C.M., Beam, K.G. J. Biol. Chem. (2000) [Pubmed]
  13. Imaging of Ca2+ transients induced in Paramecium cells by a polyamine secretagogue. Klauke, N., Plattner, H. J. Cell. Sci. (1997) [Pubmed]
  14. Chelation of intracellular calcium prevents mesangial cell proliferative responsiveness. Whiteside, C., Munk, S., Zhou, X., Miralem, T., Templeton, D.M. J. Am. Soc. Nephrol. (1998) [Pubmed]
  15. Extracellular Mg2+ induces an intracellular Ca2+ wave during oocyte activation in the marine shrimp Sicyonia ingentis. Lindsay, L.L., Hertzler, P.L., Clark, W.H. Dev. Biol. (1992) [Pubmed]
  16. Elevation of intracellular calcium in smooth muscle causes endothelial cell generation of NO in arterioles. Dora, K.A., Doyle, M.P., Duling, B.R. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  17. Acoustic overstimulation increases outer hair cell Ca2+ concentrations and causes dynamic contractions of the hearing organ. Fridberger, A., Flock, A., Ulfendahl, M., Flock, B. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  18. Expression of the apical conjugate export pump, Mrp2, in the polarized hepatoma cell line, WIF-B. Nies, A.T., Cantz, T., Brom, M., Leier, I., Keppler, D. Hepatology (1998) [Pubmed]
  19. Calcium-containing organelles display unique reactivity to chemical stimulation in cultured hippocampal neurons. Korkotian, E., Segal, M. J. Neurosci. (1997) [Pubmed]
  20. Involvement of calcium in macrophage leukotriene release during experimental cirrhosis. Alric, L., Pinelli, E., Carrera, G., Vinel, J.P., Beraud, M., Duffaut, M., Pascal, J.P., Pipy, B. Hepatology (1996) [Pubmed]
  21. Dual signal transduction through delta opioid receptors in a transfected human T-cell line. Sharp, B.M., Shahabi, N.A., Heagy, W., McAllen, K., Bell, M., Huntoon, C., McKean, D.J. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  22. Pancreastatin increases free cytosolic Ca2+ in rat hepatocytes, involving both pertussis-toxin-sensitive and -insensitive mechanisms. Sánchez-Margalet, V., Lucas, M., Goberna, R. Biochem. J. (1993) [Pubmed]
  23. Luminal Ca2+ promoting spontaneous Ca2+ release from inositol trisphosphate-sensitive stores in rat hepatocytes. Missiaen, L., Taylor, C.W., Berridge, M.J. J. Physiol. (Lond.) (1992) [Pubmed]
  24. Mitochondrial calcium transients in adult rabbit cardiac myocytes: inhibition by ruthenium red and artifacts caused by lysosomal loading of Ca(2+)-indicating fluorophores. Trollinger, D.R., Cascio, W.E., Lemasters, J.J. Biophys. J. (2000) [Pubmed]
  25. GABA induces a unique rise of [Ca]i in cultured rat hippocampal neurons. Segal, M. Hippocampus. (1993) [Pubmed]
  26. Metastasis suppression by breast cancer metastasis suppressor 1 involves reduction of phosphoinositide signaling in MDA-MB-435 breast carcinoma cells. DeWald, D.B., Torabinejad, J., Samant, R.S., Johnston, D., Erin, N., Shope, J.C., Xie, Y., Welch, D.R. Cancer Res. (2005) [Pubmed]
  27. MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells. Cantz, T., Nies, A.T., Brom, M., Hofmann, A.F., Keppler, D. Am. J. Physiol. Gastrointest. Liver Physiol. (2000) [Pubmed]
  28. A calcium-dependent feedback mechanism participates in shaping single NMDA miniature EPSCs. Umemiya, M., Chen, N., Raymond, L.A., Murphy, T.H. J. Neurosci. (2001) [Pubmed]
  29. Evaluation of SDF-1/CXCR4-induced Ca2+ signaling by fluorometric imaging plate reader (FLIPR) and flow cytometry. Princen, K., Hatse, S., Vermeire, K., De Clercq, E., Schols, D. Cytometry. Part A : the journal of the International Society for Analytical Cytology. (2003) [Pubmed]
  30. The multidrug resistance protein 1: a functionally important activation marker for murine Th1 cells. Prechtl, S., Roellinghoff, M., Scheper, R., Cole, S.P., Deeley, R.G., Lohoff, M. J. Immunol. (2000) [Pubmed]
  31. Stimulation of capacitative calcium entry in HL-60 cells by nanosecond pulsed electric fields. White, J.A., Blackmore, P.F., Schoenbach, K.H., Beebe, S.J. J. Biol. Chem. (2004) [Pubmed]
  32. Calcium transients associated with the T type calcium current in myotubes. García, J., Beam, K.G. J. Gen. Physiol. (1994) [Pubmed]
  33. CD18/ICAM-1-dependent nitric oxide production of Kupffer cells as a cause of mitochondrial dysfunction in hepatoma cells: influence of chronic alcohol feeding. Kurose, I., Higuchi, H., Watanabe, N., Miura, S., Tomita, K., Yonei, Y., Takaishi, M., Zeki, S., Nakamura, T., Saito, H., Kato, S., Ishii, H. Free Radic. Biol. Med. (1997) [Pubmed]
  34. A midregion parathyroid hormone-related peptide mobilizes cytosolic calcium and stimulates formation of inositol trisphosphate in a squamous carcinoma cell line. Orloff, J.J., Ganz, M.B., Nathanson, M.H., Moyer, M.S., Kats, Y., Mitnick, M., Behal, A., Gasalla-Herraiz, J., Isales, C.M. Endocrinology (1996) [Pubmed]
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