Disease relevance of NSC 79102

• In addition, we demonstrated marked growth stimulation with testosterone and dehydrotestosterone using different androgen-receptor-positive small-cell lung cancer cell lines [1].
• Dehydrotestosterone penile stimulation in hypospadias surgery [2].
• Growth of human prostate carcinomas with and without hormone alpha- dehydrotestosterone in nude mice [3].
• For men, interest has centred on the use of 5 alpha-reductase inhibitors to block the prostatic conversion of testosterone to dehydrotestosterone and potentially inhibit the development of prostate cancer [4].
• Serum concentrations of gonadotropins, testosterone and dehydrotestosterone were determined in patients receiving conventional endocrine therapy for advanced metastatic adenocarcinoma of prostate [5].

High impact information on NSC 79102

• Identification of some important metabolites of boldenone in urine and feces of cattle by gas chromatography-mass spectrometry [6].
• The presence of the double bond (C1-C2) in 1-dehydrotestosterone did not influence the position of hydroxylation, but the product with additional C14-C15 double bond (at the same site as hydroxylation) was formed [7].
• Urine samples were determined directly after dilution, omitting hydrolysis of boldenone conjugates [8].
• An enzyme linked immunosorbent assay (ELISA) was developed to detect the anabolic steroid boldenone in equine blood and urine [8].
• In our laboratory, the invertebrate Neomysis integer (Crustacea, Mysidacea) was used since 2004 as an alternative model for the partial replacement of vertebrate animals in metabolisation studies with illegal growth promotors and veterinary drugs, e.g. boldenone [9].

Associations of NSC 79102 with other chemical compounds

• An HPLC separation of a complex mixture containing 13 urinary anabolics and corticoids, and boldenone and bolasterone (synthetic anabolics) has been carried out [10].
• The first results indicate that maggots of L. sericata are able to convert phytosterols and -stanols, nowadays in substantial amounts added to animal feed, into androsta-1,4-diene-3,17-dione (ADD), the precursor of boldenone, at a yield of 0.10-0.14% (p<0.001, significance compared to endogenous excretion of maggots) but not to boldenone itself [9].
• Boldenone sulfate, boldenone glucuronide and their C17-epimers were synthesised as reference standards in our lab and a method was developed for their detection in a horse urine matrix [11].

Gene context of NSC 79102

• Boldenone (I) and four metabolites were identified after hydrolysis of the urine with beta-glucuronidase: 5 beta-androst-1-en-17 beta-ol-3-one (II), 5 beta-androst-1-ene-3 alpha, 17 beta-diol (III), 5 beta-androst-1-en-3 alpha-ol-17-one (IV) and 5 beta-androst-1-en-6 beta-ol-3,17-dione (V) [12].

Analytical, diagnostic and therapeutic context of NSC 79102

• Total immunoreactivity in the supernatant was determined directly by use of an ELISA, and individual boldenone concentration was measured by use of a high-performance liquid chromatography (HPLC)/ELISA after solvent extraction of the aqueous phase [13].
• A systematic optimization of the HPLC separation of a complex mixture containing urinary steroids (anabolics and corticoids), boldenone and bolasterone (synthetic anabolics) by micellar liquid chromatography has been carried out [14].
• Studies related to the metabolism of anabolic steroids in the horse: the metabolism of 1-dehydrotestosterone and the use of fast atom bombardment mass spectrometry in the identification of steroid conjugates [15].
• Unchanged 1-dehydrotestosterone was the only component identified by gas chromatography mass spectrometry after solvolysis of the sulphoconjugates [15].
• Urinary metabolites of boldenone (androsta-1,4-dien-17 beta-ol-3-one) following oral administration of boldenone (doses from 11 to 80 mg) to man were isolated from urine via XAD-2 adsorption and enzymatic hydrolysis with beta-glucuronidase from Escherichia coli [12].

References