The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Chemical Compound Review

AC1NT1A0     (5R)-8-methyl-8- azabicyclo[3.2.1]octan-3-one

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Tropinone


High impact information on Tropinone

  • In the synthesis of tropane alkaloids in several medicinal plants, the TRs reduce a carbonyl group of an alkaloid intermediate, tropinone, to hydroxy groups with different diastereomeric configurations [3].
  • A modeling study indicated that these charged residues play a major role in controlling the binding orientation of tropinone within the substrate binding site, thereby determining the stereospecificity of the reaction product [3].
  • Tropinone reductase-II (TR-II) catalyzes the NADPH-dependent reduction of the carbonyl group of tropinone to a beta-hydroxyl group [4].
  • Structure of tropinone reductase-II complexed with NADP+ and pseudotropine at 1.9 A resolution: implication for stereospecific substrate binding and catalysis [4].
  • The recombinant TRII protein expressed in Escherichia coli catalyzes pseudotropine formation from tropinone with a Km value, a pH optimum, substrate and co-substrate preferences similar to those reported for the TRII enzymes from other Solanaceae species [5].

Biological context of Tropinone


Gene context of Tropinone


Analytical, diagnostic and therapeutic context of Tropinone


  1. Opposite stereospecificity of two tropinone reductases is conferred by the substrate-binding sites. Nakajima, K., Hashimoto, T., Yamada, Y. J. Biol. Chem. (1994) [Pubmed]
  2. Tropine dehydrogenase: purification, some properties and an evaluation of its role in the bacterial metabolism of tropine. Bartholomew, B.A., Smith, M.J., Long, M.T., Darcy, P.J., Trudgill, P.W., Hopper, D.J. Biochem. J. (1995) [Pubmed]
  3. Crystal structures of two tropinone reductases: different reaction stereospecificities in the same protein fold. Nakajima, K., Yamashita, A., Akama, H., Nakatsu, T., Kato, H., Hashimoto, T., Oda, J., Yamada, Y. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  4. Structure of tropinone reductase-II complexed with NADP+ and pseudotropine at 1.9 A resolution: implication for stereospecific substrate binding and catalysis. Yamashita, A., Kato, H., Wakatsuki, S., Tomizaki, T., Nakatsu, T., Nakajima, K., Hashimoto, T., Yamada, Y., Oda, J. Biochemistry (1999) [Pubmed]
  5. Molecular cloning, expression and characterization of tropinone reductase II, an enzyme of the SDR family in Solanum tuberosum (L.). Keiner, R., Kaiser, H., Nakajima, K., Hashimoto, T., Dräger, B. Plant Mol. Biol. (2002) [Pubmed]
  6. Tropinone reductases, enzymes at the branch point of tropane alkaloid metabolism. Dräger, B. Phytochemistry (2006) [Pubmed]
  7. Insight into the molecular evolution of two tropinone reductases. Nakajima, K., Oshita, Y., Yamada, Y., Hashimoto, T. Biosci. Biotechnol. Biochem. (1999) [Pubmed]
  8. The reduction of tropinone in Datura stramonium root cultures by two specific reductases. Portsteffen, A., Dräger, B., Nahrstedt, A. Phytochemistry (1994) [Pubmed]
  9. Site-directed mutagenesis of putative substrate-binding residues reveals a mechanism controlling the different stereospecificities of two tropinone reductases. Nakajima, K., Kato, H., Oda, J., Yamada, Y., Hashimoto, T. J. Biol. Chem. (1999) [Pubmed]
  10. Crystallization and preliminary crystallographic study of tropinone reductase II from Datura stramonium. Yamashita, A., Nakajima, K., Kato, H., Hashimoto, T., Yamada, Y., Oda, J. Acta Crystallogr. D Biol. Crystallogr. (1998) [Pubmed]
WikiGenes - Universities