High impact information on NSC10246

• We observed that reducing the length of molecules able to cross-link amino groups decreased the efficiency of cross-linking with the 100-kDa protein and only the 85-kDa protein was cross-linked with erythropoietin using 1,5-difluoro-2,4-dinitrobenzene [1].
• When phosphorylase kinase from rabbit skeletal muscle was activated by phosphorylation and then cross-linked with 1,5-difluoro-2,4-dinitrobenzene at pH 6.8, dimers of beta subunits were formed that were not observed during cross-linking of nonphosphorylated enzyme under the same conditions [2].
• Incubation of human Glu-plasminogen with 1,5-difluoro-2,4-dinitrobenzene leads to the specific intra-molecular cross-linking of the kringle 1+2+3 region and the light (B) chain region of plasminogen [3].
• In the presence of 1,5-difluoro-2,4-dinitrobenzene, formation of a cross-linked alpha, beta-dimer was affected specifically by K+ + ATP or enzyme phosphorylation [4].
• Haptoglobin-hemoglobin complex has been cross-linked using the bifunctional reagent 1,5-difluoro-2,4-dinitrobenzene [5].

Biological context of NSC10246

• Affinity labeling of binding sites on gill membranes with 125I-labeled ET-1 and the cross-linking reagent 1,5-difluoro-2,4-dinitrobenzene revealed one major protein with an M(r) of 58,000, similar to that found in mammals [6].
• The troponin complex isolated from rabbit skeletal muscle was cross-linked with 1,3-difluoro-4,6-dinitrobenzene, subjected to tryptic hydrolysis, and the labelled peptides were separated [7].

Anatomical context of NSC10246

• Microsomal membranes from rat liver were treated with the cross-linking reagent 1,5-difluoro-2,4-dinitrobenzene (DFDNB) [8].
• Crosslinking of sarcoplasmic reticulum ATPase protein with 1,5-difluoro 2,4-dinitrobenzene [9].
• Proteins S7, S10, S13, S21, S22 and S27 in the small subunit and L2/3, L5, L10/12, L19/20, L22, L23, L36/37, L42 and L43' in the large subunit are labelled when cross linked to [14C]spermidine using 1,5-difluoro 2,4-dinitrobenzene and are good candidates to be RNA-binding proteins in ribosomes from Saccharomyces cerevisiae [10].

Associations of NSC10246 with other chemical compounds

• Leukotriene C4 (LTC4) has been conjugated with Bovine Serum Albumin (BSA) and Hemocyanin from Giant Keyhole Limpets (KLH) using 1,5-difluoro-2,4-dinitrobenzene as coupling agent [11].
• Rabbit muscle glycogen phosphorylase b (1,4-alpha-D-glucan:orthophosphate alpha-D-glucosyltransferase, EC 2.4.1.1) was inactivated by 1,5-difluoro-2,4-dinitrobenzene at pH 7.6 at a rate much faster than by 1-fluoro-2,4-dinitrobenzene [12].
• [125I] Thyroxine has been covalently bound to the thyroxine binding site in thyroxine-binding globulin by reaction with the bifunctional reagent, 1,5-difluoro-2,4-dinitrobenzene [13].

Gene context of NSC10246

• Ligands promoting phosphorylation of the Na+ + K+-stimulated ATPase decreased the number of thiol groups bridged by 1,5-difluoro-2,4-dinitrobenzene and by 4,4'-difluoro-3,3'-dinitrodiphenyl sulphone [0.7--1.0 nm (7--10 A) span] [14].
• The amide (III) was coupled with Bovine Serum Albumin using 1,5-difluoro-2,4-dinitrobenzene as coupling agent [15].
• Two-step modification of aspartate aminotransferase with 1,5-difluoro-2,4-dinitrobenzene. Cross-link localization [16].
• Cytochrome c oxidase was treated with 1,5-difluoro-2,4-dinitrobenzene at molar ratios (DFDNB:oxidase) varying from 5 to 625 [17].
• To test this possibility, several TRH-Dno derivatives were prepared by reaction of TRH with equimolar amounts of 1,5-difluoro-2,4-dinitrobenzene yielding Nim-(5-fluoro-2,4-dinitrophenyl)TRH [18].

Analytical, diagnostic and therapeutic context of NSC10246

• With immunoblotting, a minor band of high molecular mass (greater than 500 kDa) was also detected and this form increased after the cells were treated with chemical cross-linker, 1,5-difluoro-2,4-dinitrobenzene [19].

References