Disease relevance of NSC367089

• Both enzymes are first apparent histochemically (using the appropriate naphthol AS-BI substrates) in the atrophic type I fibres of, for example, 3--4 weeks-old dystrophic hamsters, at which age type II fibres (in the Johnson & Pearse nomenclature) contain neither enzyme and a myopathy is undetectable by standard clinical criteria [1].

High impact information on NSC367089

• Diazotized p-(acetoxymercuric) aniline in a post-coupling procedure, using naphthol AS-BI phosphate as substrate, yielded a fine particulate reaction product within vacuoles, intercellular spaces, multivesicular bodies and at various sites throughout the cytoplasm of pea root cells and differentiating mung bean protoxylem cells [2].
• Transport inhibition studies revealed competition between three glucuronides: phenolphthalein glucuronide, estradiol 17-glucuronide and naphthol AS-BI glucuronide indicating that they share a common transporter in the endoplasmic reticulum membrane [3].
• A quantitative cytochemical study of isolated hepatocytes from young, middle-aged and old adult rats has shown a doubling of acid naphthol AS-BI phosphatase activity as the hepatocytes shift from the diploid to the tetraploid state, and an overall increase in activity in cells from old animals [4].
• Comments on the cytochemical techniques using naphthol AS-BI substrates and hexazonium pararosanilin for the demonstration of acid phosphatase, beta-glucuronidase and N-acetyl-beta-glucosaminidase activities [5].
• This technique involves applying naphthol AS-BI phosphate to the surface of intact tissue where it is hydrolysed by alkaline phosphatase present in the luminal membrane of the epithelial cells [6].

Biological context of NSC367089

• After stimulation of guinea pigs with estradiol to increase their Kurloff cell number, spleen imprints were prepared in order to detect non-specific acid phosphatase (AcPase) activity by light microscopic cytochemistry using naphthol AS-BI phosphate as substrate and pararosanilin or fast garnet GBC as coupler [7].

Anatomical context of NSC367089

• Cryostat sections of unfixed muscle mounted on dry dialysis membranes are first incubated for 1-4 at 37 degrees C on a gelled medium containing 4 mM naphthol AS-BI phosphate buffered at pH 5 [8].
• Naphthol-As-Bi-phosphate (0.025-1.5 mM) is employed as substrate and Fast Blue B as coupling reagent, and the resulting azo-dye in the brush border membrane has an absorbance maximum at lambda 550 nm [9].
• The simultaneous coupling azo dye method using the substrates sodium alpha-naphthyl phosphate and Naphthol AS-BI phosphate, resulted in the demonstration of alkaline phosphatase activity in the surface epithelial cells, and the middle and upper crypts, of normal and transitional mucosa [10].

Associations of NSC367089 with other chemical compounds

• Alkaline phosphatase activity was localized by using naphthol AS-BI phosphate as substrate in 0.125 M 2-amino-2-methyl-1-propanol buffer containing 0.625 mM MgCl2 (pH 8.9) [11].

References