Disease relevance of PREB

• RT-PCR analysis of human brain tumor RNA samples suggested region-specific expression of PREB transcripts in brain [1].

Psychiatry related information on PREB

• We propose that PREB has a role during human development and that abnormal dosage of this transcription factor may be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p [2].

High impact information on PREB

• We suggest that Sec12p participates actively in the growth of COPII vesicles by charging new Sar1p-GTP molecules that insert at the boundary between a bud and the surrounding endoplasmic reticulum membrane [3].
• In order to recapitulate a more authentic, GTP-dependent budding event, we introduced the Sar1p nucleotide exchange catalyst, Sec12p, and evaluated the dynamics of coat assembly and disassembly by light scattering and tryptophan fluorescence measurements [3].
• By contrast, specific inhibition of endoplasmic reticulum export via overexpression of Sec12p or coexpression of a GTP-restricted form of Sar1p inhibits the anterograde transport of either cargo molecule in a similar manner [4].
• However, three different rodent non-pituitary cell lines yielded a common shifted band, corresponding to a DNA sequence-specific PRE-binding protein (PREB) [5].
• That PREB and ssPREB are distinct proteins was confirmed by the inability of their DNA binding sites to cross-compete binding of the proteins [5].

Biological context of PREB

• PREB transcripts were detected in all human tissues examined, but the observation of tissue-specific multiple transcript patterns suggests the possibility of tissue-specific alternative splicing [1].
• Cloning and characterization of human PREB; a gene that maps to a genomic region associated with trisomy 2p syndrome [2].
• PREB was mapped to human Chromosome 2p23, a region of the genome associated with partial trisomy 2p syndrome [2].
• We therefore examined the role of PREB in insulin gene expression [6].
• MATERIALS AND METHODS: To analyse the effects of PREB on insulin gene transcription, we employed the luciferase reporter gene assay and electrophoretic mobility shift assay (EMSA) [6].

Anatomical context of PREB

• Western and immunocytochemical analysis implied that PREB accumulates specifically in GH3 cell nuclei [1].
• PREB is located not only in the anterior pituitary but also in pancreas; however its role in the pancreas is not known [6].
• RESULTS: PREB was located mainly in nuclei of rat pancreatic beta cells and its cell line, INS-1 [6].

Associations of PREB with chemical compounds

• The addition of glucose to the cells increased PREB expression [6].

Regulatory relationships of PREB

• AIMS/HYPOTHESIS: Prolactin regulatory element binding (PREB) protein has been identified as a factor that regulates prolactin promoter activity in rat anterior pituitary [6].

Other interactions of PREB

• In the INS-1 cells, co-expression of PREB and the insulin promoter induced activity of the latter [6].

References