Disease relevance of TNIP1

• Naf1 mRNA levels were higher in synovial biopsies from patients with active RA and seronegative arthropathy than in those from patients with osteoarthritis [1].
• Using specimens from 29 acute myeloid leukemia (AML) patients, we detected a high frequency of allelic loss on DNA at STS marker D5S2014 near the Naf1 gene [2].
• Naf1 also binds to Nef, which plays a key role in acquired immunodeficiency syndrome pathogenesis and HIV-1 virus replication [2].

High impact information on TNIP1

• By interrogating the site of H/ACA RNA transcription, we dissected their biogenesis in single cells and delineated the role of the non-core protein NAF1 in the process [3].
• hNaf1 is required for accumulation of human box H/ACA snoRNPs, scaRNPs, and telomerase [4].
• HeLa hNaf1 associates with dyskerin and hNop10 as well as box H/ACA scaRNAs, snoRNAs, and hTR [4].
• These observations demonstrate a subtle equilibrium between NAF1 expression levels and the availability of NAF1 nuclear binding sites [5].
• Identification of Naf1/ABIN-1 among TNF-alpha-induced expressed genes in human synoviocytes using oligonucleotide microarrays [1].

Biological context of TNIP1

• Upon EGF treatment, Naf1 alpha was phosphorylated by the EGF/MEK/ERK2 signal transduction pathway [6].
• A new ERK2 binding protein, Naf1, attenuates the EGF/ERK2 nuclear signaling [6].
• Naf1 mRNA consists of 18 exons and multiple splice variants have been reported; two isoforms for exon 1, deletion of exon 2 and isoforms alpha and beta for exon 18 [2].

Anatomical context of TNIP1

• The Naf1 mRNA is ubiquitously expressed in human tissues with strong expression in peripheral blood lymphocytes and spleen [7].
• Naf1 alpha2 was widely expressed in PBMNCs from healthy adults, AML blasts and cell lines, suggesting it is the main transcript of the Naf1 gene [2].

Other interactions of TNIP1

• These results suggest that A20 and ABIN-1 probably act independently of their mutual interaction [8].
• Structure-function analysis of the A20-binding inhibitor of NF-kappa B activation, ABIN-1 [8].
• To elucidate the details of the mechanism of ERK1/2 signaling in human cells, we isolated Nef-associated factor 1 alpha (Naf1 alpha) by a yeast two-hybrid system, which bound to human ERK2 [6].
• Naf1 overexpression increased cell surface CD4 expression [7].
• In peripheral blood mononucleocytes (PBMNCs) from healthy adults, almost no expression of full-length Naf1 (Naf1FL), Naf1 alpha3 and beta3 were observed [2].

Analytical, diagnostic and therapeutic context of TNIP1

• In clinical AML patients, the expression of Naf1 alpha3 was much higher at diagnosis than on remission after chemotherapy, suggesting the possible dominant negative effect of Naf1 alpha3 [2].

References