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Gene Review

napF  -  ferredoxin-type protein

Escherichia coli CFT073

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Disease relevance of napF


High impact information on napF

  • Taking into account the cytoplasmic localization of NapF, the presence of labile Fe-S clusters in the protein, the napF(-) strain phenotype, and the NapF-dependent reactivation of the 2,2'-dipyridyl-treated NapA, we propose a role for NapF in assembling the [4Fe-4S] center of the catalytic subunit NapA [1].
  • The finding that IscR repressed expression of the hyaA, hybO and napF promoters specifically under aerobic growth conditions suggests a new mechanism to explain their upregulation under anaerobic growth conditions [3].
  • NapF, NapG and NapH were shown to play no role in electron transfer from menaquinol to the NapAB complex but, in the Ubi+Men- background, deletion of napF, napGH or napFGH all resulted in total loss of nitrate-dependent growth [4].
  • The R. sphaeroides napF gene product is a 16.4 kDa protein with four cysteine clusters that probably bind four [4Fe-4S] centres [5].
  • Previous studies have shown that the napF operon control region directs synthesis of two transcripts whose 5' ends differ by about 3 nucleotides [6].

Biological context of napF

  • Utilizing a napF-lacZ operon fusion, we demonstrate that napF gene expression is impaired in strain defective for the molybdate-responsive ModE transcription factor [7].
  • The upstream ModE regulatory site functions to override nitrate control of napF gene expression when the essential enzyme component, molybdate, is limiting in the cell environment [7].
  • These results establish the unconventional napF operon control region architecture, in which the major promoter P1 is activated by the Fnr protein bound to a site centered at -64.5 with respect to the transcription initiation site, working in conjunction with the phospho-NarP protein bound to a site centered at -44.5 [6].
  • The napF operon control region exhibits unusual organization of DNA binding sites for the transcription regulators Fnr and NarP, which activate transcription in response to anaerobiosis and nitrate, respectively [6].
  • The transcriptional regulation of napF was successfully reproduced in vitro by using a supercoiled plasmid template and purified Fnr, NarL, and NarP proteins [8].

Associations of napF with chemical compounds


Other interactions of napF

  • In contrast, deletion of the upstream ModE region site rendered napF expression independent of modE [7].

Analytical, diagnostic and therapeutic context of napF


  1. NapF is a cytoplasmic iron-sulfur protein required for Fe-S cluster assembly in the periplasmic nitrate reductase. Olmo-Mira, M.F., Gavira, M., Richardson, D.J., Castillo, F., Moreno-Vivián, C., Roldán, M.D. J. Biol. Chem. (2004) [Pubmed]
  2. The napF and narG nitrate reductase operons in Escherichia coli are differentially expressed in response to submicromolar concentrations of nitrate but not nitrite. Wang, H., Tseng, C.P., Gunsalus, R.P. J. Bacteriol. (1999) [Pubmed]
  3. IscR-dependent gene expression links iron-sulphur cluster assembly to the control of O-regulated genes in Escherichia coli. Giel, J.L., Rodionov, D., Liu, M., Blattner, F.R., Kiley, P.J. Mol. Microbiol. (2006) [Pubmed]
  4. Roles of NapF, NapG and NapH, subunits of the Escherichia coli periplasmic nitrate reductase, in ubiquinol oxidation. Brondijk, T.H., Fiegen, D., Richardson, D.J., Cole, J.A. Mol. Microbiol. (2002) [Pubmed]
  5. Periplasmic nitrate-reducing system of the phototrophic bacterium Rhodobacter sphaeroides DSM 158: transcriptional and mutational analysis of the napKEFDABC gene cluster. Reyes, F., Gavira, M., Castillo, F., Moreno-Vivián, C. Biochem. J. (1998) [Pubmed]
  6. Dual overlapping promoters control napF (periplasmic nitrate reductase) operon expression in Escherichia coli K-12. Stewart, V., Bledsoe, P.J., Williams, S.B. J. Bacteriol. (2003) [Pubmed]
  7. The molybdate-responsive Escherichia coli ModE transcriptional regulator coordinates periplasmic nitrate reductase (napFDAGHBC) operon expression with nitrate and molybdate availability. McNicholas, P.M., Gunsalus, R.P. J. Bacteriol. (2002) [Pubmed]
  8. Fnr, NarP, and NarL regulation of Escherichia coli K-12 napF (periplasmic nitrate reductase) operon transcription in vitro. Darwin, A.J., Ziegelhoffer, E.C., Kiley, P.J., Stewart, V. J. Bacteriol. (1998) [Pubmed]
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