The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

SH2D1B  -  SH2 domain containing 1B

Homo sapiens

Synonyms: EAT-2, EAT2, EWS/FLI1-activated transcript 2, SH2 domain-containing protein 1B
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of SH2D1B


High impact information on SH2D1B

  • We conclude that EAT-2 plays a role in controlling signal transduction through at least four receptors expressed on the surface of professional antigen-presenting cells [2].
  • Here we report that a second gene encoding a free SH2 domain, EAT-2, is expressed in macrophages and B lympho cytes [2].
  • The EAT-2 structure in complex with a phosphotyrosine peptide containing a sequence motif with Tyr281 of the cytoplasmic tail of CD150 is very similar to the structure of SH2D1A complexed with the same peptide [2].
  • Interestingly, overexpression of SAP, but not its homolog EAT-2, leads to a synergistic activation of nuclear factor of activating T cells (NFAT) in combination with a calcium signal in T cells [3].
  • The ability of SLAM family receptors to regulate NK cell functions seems to be largely dependent on their capacity to associate, by way of their cytoplasmic domain, with members of the SAP family of adapters, including SAP, Ewing's sarcoma-activated transcript-2 (EAT-2), and EAT-2-related transducer (ERT) [4].

Biological context of SH2D1B

  • We show that association of EAT-2 induces the phosphorylation of CRACC and that this process is partially reduced by a pharmacological inhibitor of Src kinases [5].
  • We have characterized a novel gene, EWS/FLI1 activated transcript 2 (EAT-2) that was cloned from a murine cDNA library using a differentially expressed RDA fragment [1].
  • SAP and EAT-2 define a new class of adaptor proteins composed almost exclusively of a Src homology 2 (SH2) domain [6].

Anatomical context of SH2D1B

  • SF2000, which is co-expressed with SAP in T cells, binds both SAP and EAT-2 [7].
  • EAT-2, a SAP-related adapter protein, is already detectable in resting NK cells and does not change its expression after IL-2 stimulation [8].
  • CD84 is up-regulated on a major population of human memory B cells and recruits the SH2 domain containing proteins SAP and EAT-2 [9].
  • Two such MAbs, Eat1 and Eat2 (for Extracellular Anti-TAPA1), were used to assess the expression and function of CD81 on murine lymphocytes [10].

Associations of SH2D1B with chemical compounds

  • Human EAT-2 (SH2D1B) and SLAM-associated protein (SAP) (SH2D1A) are single SH2-domain adapters, which bind to specific tyrosine residues in the cytoplasmic tail of six signaling lymphocytic activation molecule (SLAM) (SLAMF1)-related receptors [11].

Other interactions of SH2D1B

  • Moreover, EAT-2 also associates with 2B4 predominantly in resting NK cells, whereas SAP preferentially binds 2B4 upon activation [5].
  • We show that NTB-A is tyrosine phosphorylated in unstimulated human NK cells and associates with SLAM-associated protein (SAP) and EAT-2 [12].

Analytical, diagnostic and therapeutic context of SH2D1B

  • CD84 became rapidly phosphorylated on tyrosine residues following ligation with a specific monoclonal antibody and recruited the cytoplasmic adaptor proteins SAP and EAT-2 [9].


  1. EAT-2 is a novel SH2 domain containing protein that is up regulated by Ewing's sarcoma EWS/FLI1 fusion gene. Thompson, A.D., Braun, B.S., Arvand, A., Stewart, S.D., May, W.A., Chen, E., Korenberg, J., Denny, C. Oncogene (1996) [Pubmed]
  2. Structural basis for the interaction of the free SH2 domain EAT-2 with SLAM receptors in hematopoietic cells. Morra, M., Lu, J., Poy, F., Martin, M., Sayos, J., Calpe, S., Gullo, C., Howie, D., Rietdijk, S., Thompson, A., Coyle, A.J., Denny, C., Yaffe, M.B., Engel, P., Eck, M.J., Terhorst, C. EMBO J. (2001) [Pubmed]
  3. The X-linked lymphoproliferative disease gene product SAP associates with PAK-interacting exchange factor and participates in T cell activation. Gu, C., Tangye, S.G., Sun, X., Luo, Y., Lin, Z., Wu, J. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  4. NK cell regulation by SLAM family receptors and SAP-related adapters. Veillette, A. Immunol. Rev. (2006) [Pubmed]
  5. The cytotoxicity receptor CRACC (CS-1) recruits EAT-2 and activates the PI3K and phospholipase Cgamma signaling pathways in human NK cells. Tassi, I., Colonna, M. J. Immunol. (2005) [Pubmed]
  6. The SAP family of adaptors in immune regulation. Latour, S., Veillette, A. Semin. Immunol. (2004) [Pubmed]
  7. Identification and characterization of SF2000 and SF2001, two new members of the immune receptor SLAM/CD2 family. Fraser, C.C., Howie, D., Morra, M., Qiu, Y., Murphy, C., Shen, Q., Gutierrez-Ramos, J.C., Coyle, A., Kingsbury, G.A., Terhorst, C. Immunogenetics (2002) [Pubmed]
  8. Modulation of 2B4 (CD244) activity and regulated SAP expression in human NK cells. Endt, J., Eissmann, P., Hoffmann, S.C., Meinke, S., Giese, T., Watzl, C. Eur. J. Immunol. (2007) [Pubmed]
  9. CD84 is up-regulated on a major population of human memory B cells and recruits the SH2 domain containing proteins SAP and EAT-2. Tangye, S.G., van de Weerdt, B.C., Avery, D.T., Hodgkin, P.D. Eur. J. Immunol. (2002) [Pubmed]
  10. Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies. Maecker, H.T., Todd, S.C., Kim, E.C., Levy, S. Hybridoma (2000) [Pubmed]
  11. Identification and characterization of two related murine genes, Eat2a and Eat2b, encoding single SH2-domain adapters. Calpe, S., Erdos, E., Liao, G., Wang, N., Rietdijk, S., Simarro, M., Scholtz, B., Mooney, J., Lee, C.H., Shin, M.S., Rajnavölgyi, E., Schatzle, J., Morse, H.C., Terhorst, C., Lanyi, A. Immunogenetics (2006) [Pubmed]
  12. Molecular analysis of NTB-A signaling: a role for EAT-2 in NTB-A-mediated activation of human NK cells. Eissmann, P., Watzl, C. J. Immunol. (2006) [Pubmed]
WikiGenes - Universities