The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

rci  -  site-specific recombinase

Escherichia coli

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of rci

 

High impact information on rci

  • The catalytic domain of lambda site-specific recombinase [6].
  • To extend the utility of the reporter gene fusion approach to such studies, we have constructed a gene expression reporter cassette that permits the generation of transcriptional fusions to tnpR encoding resolvase, a site-specific recombinase of the transposable element gamma delta [7].
  • Our study of how a site-specific recombinase recognizes an inverted-repeat binding site indicates that such sequence asymmetry can be functionally important [8].
  • By DNA sequence analysis, a 252 bp invertible element was found in the intergenic region between mrpl, the putative site-specific recombinase gene, and mrpA, the primary structural subunit gene [9].
  • We report plastid marker-gene excision with a transiently expressed CRE, site-specific recombinase [10].
 

Biological context of rci

  • The DNA sequences of the shufflon region and the rci gene of IncI2 plasmid R721 were determined [11].
  • The rci-mediated DNA inversion occurred between two synthetic inverted repeats, indicating that the 19-bp inverted repeat sequences are the sole elements required in cis for the shufflon system [12].
  • Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in groups [12].
  • In contrast, the shufflon multiple inversion system of plasmid R64 consists of seven sfx recombination sites, which separate four invertible DNA segments, and the rci gene encoding a site-specific recombinase of the integrase family [13].
  • Site-specific recombination mediated by the product of the rci gene between any two inverted repeats results in a complex DNA rearrangement [14].
 

Analytical, diagnostic and therapeutic context of rci

References

  1. Anabaena xisF gene encodes a developmentally regulated site-specific recombinase. Carrasco, C.D., Ramaswamy, K.S., Ramasubramanian, T.S., Golden, J.W. Genes Dev. (1994) [Pubmed]
  2. A site-specific recombinase is required for competitive root colonization by Pseudomonas fluorescens WCS365. Dekkers, L.C., Phoelich, C.C., van der Fits, L., Lugtenberg, B.J. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  3. Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination. Parsons, R.L., Prasad, P.V., Harshey, R.M., Jayaram, M. Mol. Cell. Biol. (1988) [Pubmed]
  4. Relating primary structure to function in the Escherichia coli XerD site-specific recombinase. Spiers, A.J., Sherratt, D.J. Mol. Microbiol. (1997) [Pubmed]
  5. Molecular genetics of the chloramphenicol-resistance transposon Tn4451 from Clostridium perfringens: the TnpX site-specific recombinase excises a circular transposon molecule. Bannam, T.L., Crellin, P.K., Rood, J.I. Mol. Microbiol. (1995) [Pubmed]
  6. The catalytic domain of lambda site-specific recombinase. Tirumalai, R.S., Healey, E., Landy, A. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  7. Use of genetic recombination as a reporter of gene expression. Camilli, A., Beattie, D.T., Mekalanos, J.J. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  8. Cooperative binding of Tn3 resolvase monomers to a functionally asymmetric binding site. Blake, D.G., Boocock, M.R., Sherratt, D.J., Stark, W.M. Curr. Biol. (1995) [Pubmed]
  9. In vivo phase variation of MR/P fimbrial gene expression in Proteus mirabilis infecting the urinary tract. Zhao, H., Li, X., Johnson, D.E., Blomfield, I., Mobley, H.L. Mol. Microbiol. (1997) [Pubmed]
  10. Plastid marker-gene excision by transiently expressed CRE recombinase. Lutz, K.A., Bosacchi, M.H., Maliga, P. Plant J. (2006) [Pubmed]
  11. Nucleotide sequence of the R721 shufflon. Kim, S.R., Komano, T. J. Bacteriol. (1992) [Pubmed]
  12. Analysis of DNA inversions in the shufflon of plasmid R64. Gyohda, A., Funayama, N., Komano, T. J. Bacteriol. (1997) [Pubmed]
  13. Structure and function of the shufflon in plasmid R64. Gyohda, A., Furuya, N., Ishiwa, A., Zhu, S., Komano, T. Adv. Biophys. (2004) [Pubmed]
  14. Mating variation by DNA inversions of shufflon in plasmid R64. Komano, T., Kim, S.R., Yoshida, T. Adv. Biophys. (1995) [Pubmed]
  15. Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system. Meyer-Leon, L., Gates, C.A., Attwood, J.M., Wood, E.A., Cox, M.M. Nucleic Acids Res. (1987) [Pubmed]
  16. Molecular cloning, nucleotide sequence, and function of a site-specific recombinase encoded in the major 'pathogenicity island' of Salmonella typhi. Zhang, X.L., Morris, C., Hackett, J. Gene (1997) [Pubmed]
 
WikiGenes - Universities