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Gene Review

rci  -  site-specific recombinase

Escherichia coli

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Disease relevance of rci


High impact information on rci

  • The catalytic domain of lambda site-specific recombinase [6].
  • To extend the utility of the reporter gene fusion approach to such studies, we have constructed a gene expression reporter cassette that permits the generation of transcriptional fusions to tnpR encoding resolvase, a site-specific recombinase of the transposable element gamma delta [7].
  • Our study of how a site-specific recombinase recognizes an inverted-repeat binding site indicates that such sequence asymmetry can be functionally important [8].
  • By DNA sequence analysis, a 252 bp invertible element was found in the intergenic region between mrpl, the putative site-specific recombinase gene, and mrpA, the primary structural subunit gene [9].
  • We report plastid marker-gene excision with a transiently expressed CRE, site-specific recombinase [10].

Biological context of rci

  • The DNA sequences of the shufflon region and the rci gene of IncI2 plasmid R721 were determined [11].
  • The rci-mediated DNA inversion occurred between two synthetic inverted repeats, indicating that the 19-bp inverted repeat sequences are the sole elements required in cis for the shufflon system [12].
  • Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in groups [12].
  • In contrast, the shufflon multiple inversion system of plasmid R64 consists of seven sfx recombination sites, which separate four invertible DNA segments, and the rci gene encoding a site-specific recombinase of the integrase family [13].
  • Site-specific recombination mediated by the product of the rci gene between any two inverted repeats results in a complex DNA rearrangement [14].

Analytical, diagnostic and therapeutic context of rci


  1. Anabaena xisF gene encodes a developmentally regulated site-specific recombinase. Carrasco, C.D., Ramaswamy, K.S., Ramasubramanian, T.S., Golden, J.W. Genes Dev. (1994) [Pubmed]
  2. A site-specific recombinase is required for competitive root colonization by Pseudomonas fluorescens WCS365. Dekkers, L.C., Phoelich, C.C., van der Fits, L., Lugtenberg, B.J. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  3. Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination. Parsons, R.L., Prasad, P.V., Harshey, R.M., Jayaram, M. Mol. Cell. Biol. (1988) [Pubmed]
  4. Relating primary structure to function in the Escherichia coli XerD site-specific recombinase. Spiers, A.J., Sherratt, D.J. Mol. Microbiol. (1997) [Pubmed]
  5. Molecular genetics of the chloramphenicol-resistance transposon Tn4451 from Clostridium perfringens: the TnpX site-specific recombinase excises a circular transposon molecule. Bannam, T.L., Crellin, P.K., Rood, J.I. Mol. Microbiol. (1995) [Pubmed]
  6. The catalytic domain of lambda site-specific recombinase. Tirumalai, R.S., Healey, E., Landy, A. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  7. Use of genetic recombination as a reporter of gene expression. Camilli, A., Beattie, D.T., Mekalanos, J.J. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  8. Cooperative binding of Tn3 resolvase monomers to a functionally asymmetric binding site. Blake, D.G., Boocock, M.R., Sherratt, D.J., Stark, W.M. Curr. Biol. (1995) [Pubmed]
  9. In vivo phase variation of MR/P fimbrial gene expression in Proteus mirabilis infecting the urinary tract. Zhao, H., Li, X., Johnson, D.E., Blomfield, I., Mobley, H.L. Mol. Microbiol. (1997) [Pubmed]
  10. Plastid marker-gene excision by transiently expressed CRE recombinase. Lutz, K.A., Bosacchi, M.H., Maliga, P. Plant J. (2006) [Pubmed]
  11. Nucleotide sequence of the R721 shufflon. Kim, S.R., Komano, T. J. Bacteriol. (1992) [Pubmed]
  12. Analysis of DNA inversions in the shufflon of plasmid R64. Gyohda, A., Funayama, N., Komano, T. J. Bacteriol. (1997) [Pubmed]
  13. Structure and function of the shufflon in plasmid R64. Gyohda, A., Furuya, N., Ishiwa, A., Zhu, S., Komano, T. Adv. Biophys. (2004) [Pubmed]
  14. Mating variation by DNA inversions of shufflon in plasmid R64. Komano, T., Kim, S.R., Yoshida, T. Adv. Biophys. (1995) [Pubmed]
  15. Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system. Meyer-Leon, L., Gates, C.A., Attwood, J.M., Wood, E.A., Cox, M.M. Nucleic Acids Res. (1987) [Pubmed]
  16. Molecular cloning, nucleotide sequence, and function of a site-specific recombinase encoded in the major 'pathogenicity island' of Salmonella typhi. Zhang, X.L., Morris, C., Hackett, J. Gene (1997) [Pubmed]
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