Disease relevance of rci

• Anabaena xisF gene encodes a developmentally regulated site-specific recombinase [1].
• A site-specific recombinase is required for competitive root colonization by Pseudomonas fluorescens WCS365 [2].
• The site-specific recombinase (FLP) encoded by the yeast plasmid 2 micron circle belongs to the integrase (of phage lambda) family of recombinases [3].
• Relating primary structure to function in the Escherichia coli XerD site-specific recombinase [4].
• Molecular genetics of the chloramphenicol-resistance transposon Tn4451 from Clostridium perfringens: the TnpX site-specific recombinase excises a circular transposon molecule [5].

High impact information on rci

• The catalytic domain of lambda site-specific recombinase [6].
• To extend the utility of the reporter gene fusion approach to such studies, we have constructed a gene expression reporter cassette that permits the generation of transcriptional fusions to tnpR encoding resolvase, a site-specific recombinase of the transposable element gamma delta [7].
• Our study of how a site-specific recombinase recognizes an inverted-repeat binding site indicates that such sequence asymmetry can be functionally important [8].
• By DNA sequence analysis, a 252 bp invertible element was found in the intergenic region between mrpl, the putative site-specific recombinase gene, and mrpA, the primary structural subunit gene [9].
• We report plastid marker-gene excision with a transiently expressed CRE, site-specific recombinase [10].

Biological context of rci

• The DNA sequences of the shufflon region and the rci gene of IncI2 plasmid R721 were determined [11].
• The rci-mediated DNA inversion occurred between two synthetic inverted repeats, indicating that the 19-bp inverted repeat sequences are the sole elements required in cis for the shufflon system [12].
• Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in groups [12].
• In contrast, the shufflon multiple inversion system of plasmid R64 consists of seven sfx recombination sites, which separate four invertible DNA segments, and the rci gene encoding a site-specific recombinase of the integrase family [13].
• Site-specific recombination mediated by the product of the rci gene between any two inverted repeats results in a complex DNA rearrangement [14].

Analytical, diagnostic and therapeutic context of rci

• Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system [15].
• Molecular cloning, nucleotide sequence, and function of a site-specific recombinase encoded in the major 'pathogenicity island' of Salmonella typhi [16].

References